Effect of intravenous infusion of omega-3 and omega-6 lipid emulsions on equine monocyte fatty acid composition and inflammatory mediator production in vitro.
Abstract: The effect of intravenous administration of lipid emulsions enriched with omega-3 (n3) and omega-6 (n6) fatty acids on equine monocyte phospholipid fatty acid composition and the synthesis of inflammatory mediators in vitro was evaluated. In a randomized crossover design, horses were infused intravenously with 20% lipid emulsions containing n3 or n6 fatty acids. Monocytes were isolated from the horses before and 0 h, 8 h, 24 h, and 7 days after lipid infusion. Monocyte fatty acid analysis demonstrated incorporation of the parenteral n3 and n6 fatty acids in monocyte phospholipids immediately after infusion, with changes in the fatty acid composition persisting for up to 7 days after infusion. In vitro production of the inflammatory mediators thromboxane B2/thromboxane B3 (TXB(2/3)) and tumor necrosis factor-alpha (TNFalpha) by peripheral blood monocytes was diminished by n3 lipid infusion and was unchanged or increased by n6 lipid infusion. The results of this study demonstrate that short-term infusions of n3 and n6 fatty acid-enriched lipid emulsions alter the fatty acid composition of equine monocyte phospholipids and modify the inflammatory response of these cells in vitro. These results also support further investigation into the use of parenteral n3 fatty acids as part of the supportive therapy of patients with multiple organ dysfunction (MODS) or systemic inflammatory response syndrome (SIRS).
Publication Date: 2000-08-18 PubMed ID: 10947170DOI: 10.1097/00024382-200014020-00024Google Scholar: Lookup
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- Clinical Trial
- Comparative Study
- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
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This study examines how injected lipid emulsions rich in omega-3 and omega-6 fatty acids affect the fatty acid composition of horse monocyte cells and their inflammatory response.
Research Overview
A randomized crossover research design was used in this study where horses were administered intravenous lipid emulsions containing omega-3 or omega-6 fatty acids. The monocytes, a type of white blood cell, were then isolated from the horses at various periods to study the changes in their fatty acid composition following the infusion and their capacity to produce inflammatory mediators.
Fatty Acid Changes
- It was observed that omega-3 and omega-6 fatty acids from the administered emulsions integrated themselves into the monocyte phospholipids right after the infusion.
- Changes in the fatty acid composition in these monocyte cells sustained for up to 7 days post infusion.
Inflammatory Evaluations
- Production of inflammatory mediators- thromboxane B2/B3 and tumor necrosis factor-alpha, by peripheral blood monocytes was decreased by omega-3 lipid infusion.
- Conversely, production of these inflammatory mediators remained unchanged or even increased in response to omega-6 lipid infusion.
Research Implications
- The observed results demonstrate that short-term infusions of lipid emulsions enriched with omega-3 and omega-6 fatty acids have the ability to change the composition of fatty acids in equine monocyte cells and alter their inflammatory responses.
- This opens a new avenue of research into the use of omega-3 fatty acids for the supportive treatment of patients suffering from multiple organ dysfunction (MODS) or systemic inflammatory response syndrome (SIRS).
Cite This Article
APA
McCann ME, Moore JN, Carrick JB, Barton MH.
(2000).
Effect of intravenous infusion of omega-3 and omega-6 lipid emulsions on equine monocyte fatty acid composition and inflammatory mediator production in vitro.
Shock, 14(2), 222-228.
https://doi.org/10.1097/00024382-200014020-00024 Publication
Researcher Affiliations
- Department of Physiology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
MeSH Terms
- Animals
- Calcimycin / pharmacology
- Cells, Cultured
- Cross-Over Studies
- Emulsions
- Endotoxemia / metabolism
- Endotoxemia / veterinary
- Fatty Acids / blood
- Fatty Acids, Omega-3 / administration & dosage
- Fatty Acids, Omega-3 / pharmacology
- Fatty Acids, Omega-6
- Fatty Acids, Unsaturated / administration & dosage
- Fatty Acids, Unsaturated / pharmacology
- Female
- Gene Expression Regulation / drug effects
- Horse Diseases / metabolism
- Horses
- Infusions, Intravenous
- Ionophores / pharmacology
- Lipopolysaccharides / pharmacology
- Male
- Membrane Lipids / blood
- Monocytes / drug effects
- Monocytes / metabolism
- Phospholipids / blood
- Thromboxane B2 / analogs & derivatives
- Thromboxane B2 / biosynthesis
- Thromboxane B2 / genetics
- Thromboxanes / analogs & derivatives
- Tumor Necrosis Factor-alpha / biosynthesis
- Tumor Necrosis Factor-alpha / genetics
Citations
This article has been cited 3 times.- Wu JM, Lin MT. Effects of specific nutrients on immune modulation in patients with gastrectomy. Ann Gastroenterol Surg 2020 Jan;4(1):14-20.
- Khol-Parisini A, van den Hoven R, Leinker S, Hulan HW, Zentek J. Effects of feeding sunflower oil or seal blubber oil to horses with recurrent airway obstruction. Can J Vet Res 2007 Jan;71(1):59-65.
- Fan W, Pan M, Zheng C, Shen H, Pi D, Song Q, Liang Z, Zhen J, Pan J, Liu L, Yang Q, Zhang Y. Leonurine Inhibits Hepatic Lipid Synthesis to Ameliorate NAFLD via the ADRA1a/AMPK/SCD1 Axis. Int J Mol Sci 2024 Oct 9;25(19).
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