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Home / Equine Research Bank / Lasers Med Sci / Effect of laser soldering irradiation on covalent bonds of p...
Lasers in medical science2006; 22(1); 10-14; doi: 10.1007/s10103-006-0411-0

Effect of laser soldering irradiation on covalent bonds of pure collagen.

Abstract: Laser tissue welding and soldering is being increasingly used in the clinical setting for defined surgical procedures. The exact induced changes responsible for tensile strength are not yet fully investigated. To further improve the strength of the bonding, a better understanding of the laser impact at the subcellular level is necessary. The goal of this study was to analyze whether the effect of laser irradiation on covalent bonding in pure collagen using irradiances typically applied for tissue soldering. Pure rabbit and equine type I collagen were subjected to laser irradiation. In the first part of the study, rabbit and equine collagen were compared using identical laser and irradiation settings. In the second part of the study, equine collagen was irradiated at increasing laser powers. Changes in covalent bonding were studied indirectly using the sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) technique. Tensile strengths of soldered membranes were measured with a calibrated tensile force gauge. In the first experiment, no differences between the species-specific collagen bands were noted, and no changes in banding were found on SDS-PAGE after laser irradiation. In the second experiment, increasing laser irradiation power showed no effect on collagen banding in SDS-PAGE. Finally, the laser tissue soldering of pure collagen membranes showed virtually no determinable tensile strength. Laser irradiation of pure collagen at typical power settings and exposure times generally used in laser tissue soldering does not induce covalent bonding between collagen molecules. This is true for both rabbit and equine collagen proveniences. Furthermore, soldering of pure collagen membranes without additional cellular components does not achieve the typical tensile strength reported in native, cell-rich tissues. This study is a first step in a better understanding of laser impact at the molecular level and might prove useful in engineering of combined collagen-soldering matrix membranes for special laser soldering applications.
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Publication Date: 2006-11-07 PubMed ID: 17115238DOI: 10.1007/s10103-006-0411-0Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigated the effects of laser irradiation, generally used in medical surgical procedures, on covalent bonds in pure collagen. The results suggested that such irradiation does not induce covalent bonding between collagen molecules, whether from rabbit or equine sources, and that pure collagen membranes do not achieve typical tensile strength when soldered without additional cellular components.

Experiment Design and Procedure

  • The research was conducted in two main phases. In the first part, the scientists worked with both rabbit and equine collagen, treating them with a laser under identical conditions. The second part of the study focused only on equine collagen; however, this time around, the laser irradiation was increased progressively as part of the experiment.
  • The changes in covalent bonding were indirectly studied through the use of Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) technique, a method for separating proteins based on their electrophoretic mobility. Moreover, the tensile strengths of the soldered membranes created from collagen were measured using a calibrated tensile force gauge.

Results

  • The experimental results revealed that the specific type of collagen (rabbit or equine) did not affect the overall experiment outcome, as no notable differences were observed between the collagen bands from different species.
  • Furthermore, neither did elevating the laser power on equine collagen result in any significant alteration; the SDS-PAGE banding remained unchanged.
  • In terms of tensile strength, laser soldering of pure collagen membranes yielded almost inconclusive results. Even after the collagen had been treated with laser irradiation, the membranes did not display typical tensile strength.

Conclusions and Implications

  • The data gathered from this study indicates that laser irradiation at regular power settings and exposure durations used in laser tissue soldering does not promote covalent bonding between collagen molecules.
  • This conclusion is applicable to different types of collagen, such as those derived from rabbit and equine sources.
  • Importantly, the findings suggest that soldering pure collagen membranes without the presence of additional cellular components does not match the typical tensile strength found in natural tissues that are rich in cells.
  • Overall, this research constitutes an important step towards understanding the effects of laser intervention on a molecular level. These insights could potentially aid in the development of collagen-soldering matrix membranes for specialized laser soldering applications.

Cite This Article

APA
Constantinescu MA, Alfieri A, Mihalache G, Stuker F, Ducray A, Seiler RW, Frenz M, Reinert M. (2006). Effect of laser soldering irradiation on covalent bonds of pure collagen. Lasers Med Sci, 22(1), 10-14. https://doi.org/10.1007/s10103-006-0411-0

Publication

Lasers in medical science
ISSN: 0268-8921
NlmUniqueID: 8611515
Country: England
Language: English
Volume: 22
Issue: 1
Pages: 10-14

Researcher Affiliations

Constantinescu, Mihai A
  • Plastic Surgery Department, Inselspital, University of Bern, 3010, Bern, Switzerland.
Alfieri, Alex
    Mihalache, George
      Stuker, Florian
        Ducray, Angélique
          Seiler, Rolf W
            Frenz, Martin
              Reinert, Michael

                MeSH Terms

                • Animals
                • Collagen / radiation effects
                • Electrophoresis, Polyacrylamide Gel
                • Horses
                • Image Processing, Computer-Assisted
                • Low-Level Light Therapy
                • Rabbits
                • Temperature
                • Tensile Strength

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