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Veterinary surgery : VS2017; 46(5); 731-737; doi: 10.1111/vsu.12639

Effect of needle diameter on the viability of equine bone marrow derived mesenchymal stem cells.

Abstract: Mesenchymal stem cells (MSCs) are frequently delivered via needle injection for treatment of musculoskeletal injuries. The purpose of this study was to evaluate the effect of needle diameter on the viability of MSCs. Methods: Equine bone marrow-derived MSCs from 5 horses were suspended in PBS, and held at room temperature for 7 hours to mimic shipping conditions. Two replicate samples for each needle size (20, 22, 23, or 25-gauge [ga]) were aspirated into a 3 mL syringe and re-injected into the holding vial 3 times, to reproduce the resuspension of cells prior to injection in clinical cases. Cells were stained with fluorescein diacetate and propidium iodide to measure viability. Flow cytometry (FC) was performed to compare cell debris and intact cells between groups. Results: MSC viability was higher when cells were passed through a 20-ga rather than a 25-ga needle. Cell suspensions passed through a 20-ga needle contained a larger percentage of intact cells, compared to 25-ga samples. The percentage of debris present in cell suspensions tended to increase with decreasing needle diameter. Neither horse nor passage had a significant effect on viability. Conclusions: Cell damage is more likely when MSCs are passed through 25-ga rather than 20-ga needles. Conclusions: Use of needles larger than 25-ga is recommended to maintain the viability of MSCs injected in horses.
© 2017 The American College of Veterinary Surgeons.
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Publication Date: 2017-03-22 PubMed ID: 28328147PubMed Central: PMC5493497DOI: 10.1111/vsu.12639Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates how the diameter of the needle used for injecting mesenchymal stem cells (MSCs) affects the viability of these cells. The results suggest that larger diameter needles cause less damage to MSCs, maintaining their viability during injection.

Methodology of the Research

  • The study utilized equine bone marrow-derived MSCs from 5 horses.
  • These cells were suspended in a phosphate-buffered saline (PBS) medium and were maintained at room temperature for 7 hours. This setup was designed to replicate the shipping conditions of these cells.
  • Replicate samples for different needle sizes (20, 22, 23, or 25-gauge needles) were used. Each cell suspension was aspirated into a 3 mL syringe and re-injected into the holding vial three times. This process aimed to duplicate the resuspension of the cells prior to injection in clinical cases.
  • To measure the viability of the cells, they were stained with fluorescein diacetate and propidium iodide.
  • Flow cytometry (FC) was then used to compare cell debris and intact cells among the different groups.

Results of the Research

  • The results revealed that MSCs’ viability was higher when the cells were passed through a 20-gauge needle rather than a 25-gauge needle.
  • Cell suspensions passed through a 20-gauge needle contained a larger percentage of intact cells, compared to those passed through a 25-gauge needle.
  • The research also unveiled that the percentage of debris present in the cell suspensions tended to increase as the diameter of the needle decreased.
  • The research found no significant effect on the viability of the cells concerning the horse from which the MSCs derived or the passage through which the needle was inserted.

Conclusions of the Research

  • Based on the results, the researchers concluded that a cell may suffer more damage when MSCs are passed through a 25-gauge needle as compared to a 20-gauge needle.
  • They therefore recommended the use of needles larger than 25-gauge in order to maintain the viability of MSCs that are being injected into horses.

Cite This Article

APA
Lang HM, Schnabel LV, Cassano JM, Fortier LA. (2017). Effect of needle diameter on the viability of equine bone marrow derived mesenchymal stem cells. Vet Surg, 46(5), 731-737. https://doi.org/10.1111/vsu.12639

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 46
Issue: 5
Pages: 731-737

Researcher Affiliations

Lang, Hayley M
  • Department of Clinical Sciences, Cornell University, Ithaca, New York.
Schnabel, Lauren V
  • Department of Clinical Sciences, Cornell University, Ithaca, New York.
Cassano, Jennifer M
  • Department of Clinical Sciences, Cornell University, Ithaca, New York.
Fortier, Lisa A
  • Department of Clinical Sciences, Cornell University, Ithaca, New York.

MeSH Terms

  • Animals
  • Bone Marrow Cells / physiology
  • Cell Culture Techniques
  • Horses
  • Mesenchymal Stem Cells / physiology
  • Needles
  • Specimen Handling / instrumentation
  • Specimen Handling / veterinary

Grant Funding

  • K08 AR060875 / NIAMS NIH HHS

Conflict of Interest Statement

. No authors have a conflict of interest.

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This article includes 20 references
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Citations

This article has been cited 13 times.
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