Effect of prostaglandin F2α administration on uterine polymorphonuclear neutrophil counts in Japanese heavy draft horses.
Abstract: The objective of this study was to examine the effect of prostaglandin F2α (dinoprost) and oxytocin administration on uterine polymorphonuclear neutrophil counts in the Japanese heavy draft mare. To compare polymorphonuclear neutrophil (PMN) counts in the endometrium, a total of 162 samples were collected from 54 estruses of 47 mares (before ovulation, day 0, and day 2) using a double-guarded cytology brush. Dinoprost (PG; 5 mg) was administered intramuscularly (i.m.) only once, on day 0, whereas oxytocin (OT; 20 U i.m.) was administered three times at 12-hr intervals starting on day 0. The plasma progesterone concentrations from days 0 to 14 were not different between the non-treatment (CON), PG treatment, and OT treatment groups. The PMN counts increased in all the groups from before administration to day 0 (CON, 0.90 to 3.55; PG, 1.20 to 8.45; and OT, 0.70 to 1.70; P=0.0014, 0.0046, and 0.0073, respectively). There was a significant decrease in PMNs from day 0 to day 2 only in the PG group (P=0.0073). The pregnancy rate was not different among the CON (12/18), PG (14/18), and OT (10/18) groups. The results of this study indicate that dinoprost can reduce uterine polymorphonuclear neutrophil counts.
©2021 The Japanese Society of Equine Science.
Publication Date: 2021-12-28 PubMed ID: 35023989PubMed Central: PMC8731690DOI: 10.1294/jes.32.117Google Scholar: Lookup
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Summary
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The research examines the effects of certain hormone treatments on the number of a specific type of white blood cell (neutrophil) in the uterus of Japanese heavy draft mares. The findings show that the prostaglandin F2 treatment notably decreased these white blood cell counts.
Objective of the Study
- The main goal of this study was to investigate the impact of prostaglandin F2 (also known as dinoprost) and oxytocin on the counts of uterine polymorphonuclear neutrophils in the Japanese heavy draft mare.
Methodology
- A total of 162 samples were collected before ovulation, on day 0, and on day 2 of 54 estruses from 47 mares using a double-guarded cytology brush. This was done to compare polymorphonuclear neutrophil counts in the endometrium.
- Dinoprost was administered only once, intramuscularly on day 0, at a 5mg dose, while oxytocin was given three times at 12-hr intervals starting from day 0 with a dose of 20U.
Results
- Results showed that progesterone concentrations in the plasma between days 0 and 14 were nearly the same for the group given no treatment (CON), the dinoprost treated (PG), and oxytocin treated (OT) groups.
- The neutrophil counts rose in all the groups from before administration to day 0, and this increase was marked in each group.
- Interestingly, there was a significant decrease in neutrophils from day 0 to day 2 only in the dinoprost group, showcasing the distinct effect of this treatment.
- The pregnancy rates among the CON, PG, and OT groups did not show significant differences.
Conclusion
- The research concluded that dinoprost has the capability to reduce uterine polymorphonuclear neutrophil counts. However, it does not affect pregnancy rates among Japanese heavy draft mares, indicating that it could be a possible treatment for certain uterine conditions without affecting fertility.
Cite This Article
APA
Niikura T, Tsogtgerel M, Niikura C, Wada H, Kanzawa S, Fujiwara T, Nambo Y.
(2021).
Effect of prostaglandin F2α administration on uterine polymorphonuclear neutrophil counts in Japanese heavy draft horses.
J Equine Sci, 32(4), 117-124.
https://doi.org/10.1294/jes.32.117 Publication
Researcher Affiliations
- Hokubu Veterinary Clinical Center, Tokachi Agricultural Mutual Aid Association, Ashoro 089-3708, Japan.
- Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
- United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
- Hokubu Veterinary Clinical Center, Tokachi Agricultural Mutual Aid Association, Ashoro 089-3708, Japan.
- Hokubu Veterinary Clinical Center, Tokachi Agricultural Mutual Aid Association, Ashoro 089-3708, Japan.
- Hokubu Veterinary Clinical Center, Tokachi Agricultural Mutual Aid Association, Ashoro 089-3708, Japan.
- Hokubu Veterinary Clinical Center, Tokachi Agricultural Mutual Aid Association, Ashoro 089-3708, Japan.
- United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
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