Effect of radiosynovectomy with holmium-166 ferric hydroxide macroaggregate on adult equine cartilage.
Abstract: To analyze the effect of radiosynovectomy with holmium-166 ferric hydroxide macroaggregate (166Ho-FHMA) on articular cartilage in 6 adult horses. Methods: Arthritic changes and mechanical properties of articular cartilage were evaluated with arthroscopy and postmortem microscopic analyses. Glycosaminoglycan content was measured by safranin-O staining combined with digital densitometry, uronic acid analyses, and dimethylene blue binding assay. 35S-sulfate labeling and autoradiography were used to localize proteoglycan synthesis and to characterize proteoglycan structures using SDS-agarose gel electrophoresis. Northern hybridizations were performed to measure the mRNA levels for aggrecan and pro-a1(II) collagen in cartilage samples. Results: Histological signs of degeneration were present in the articular cartilage of both control and radiosynovectomized equine joints. Radiosynovectomy did not aggravate degenerative changes or significantly alter the matrix glycosaminoglycan content. A slightly decreased size of proteoglycan monomers was observed 2 months after 166Ho-FHMA radiosynovectomy. Tissue analysis of extracted proteoglycans revealed lower 35S incorporation after radiosynovectomy, but corresponding changes could not be observed in aggrecan mRNA levels. Transient downregulation of pro-a1(II) collagen mRNA transcription was observed 5 days after 166Ho-FHMA radiosynovectomy. Conclusions: 166Ho-FHMA treatment did not markedly affect the composition or morphology of adult articular cartilage showing mild degeneration. However, minor degradation of proteoglycan monomers and transient downregulation of pro-a1(II) collagen mRNA were observed.
Publication Date: 2004-02-05 PubMed ID: 14760804
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focuses on analyzing the impact of a specific treatment method, radiosynovectomy with holmium-166 ferric hydroxide macroaggregate (166Ho-FHMA), on adult horse joint cartilage, particularly looking at changes in arthritic conditions, cartilage structure, and relevant molecular components.
Research Method and Process
- The researchers’ approach is centered on observing changes in 6 adult horses post radiosynovectomy with 166Ho-FHMA, focusing on the articular cartilage.
- Techniques such as arthroscopy and microscopic analysis after death were used to evaluate potential arthritis changes and articular cartilage’s mechanical attributes.
- They also measured the glycosaminoglycan content—a crucial component of cartilage—using the safranin-O staining method, digital densitometry, uronic acid analyses, and dimethylene blue binding assay.
- To examine proteoglycan synthesis localization and structure (proteoglycans are key cartilage components), the researchers used techniques including 35S-sulfate labeling, autoradiography, and SDS-agarose gel electrophoresis.
- Finally, Northern hybridizations were performed to gauge the mRNA levels for aggrecan and pro-a1(II) collagen—both substantial elements in the cartilage matrix—in the cartilage samples.
Research Findings
- The study detected signs of degeneration in articular cartilage, both in control (untreated) and radiosynovectomized equine joints.
- Radiosynovectomy with 166Ho-FHMA did not further stimulate degenerative changes or considerably modify the matrix glycosaminoglycan content.
- A slight decrease in the size of proteoglycan monomers was noticed 2 months post radiosynovectomy.
- Cartilage tissue analysis showed a decrease in 35S incorporation after treatment, but no corresponding changes were noticed in aggrecan mRNA levels.
- A short-term downregulation of pro-a1(II) collagen mRNA was evident 5 days post radiosynovectomy.
Study Conclusions
- The findings affirm that 166Ho-FHMA treatment does not significantly affect the composition or morphology of adult articular cartilage, even those showing mild degeneration.
- However, minor degradation of proteoglycan monomers and a temporary downregulation of pro-a1(II) collagen mRNA were observed post treatment.
Cite This Article
APA
Mäkelä OT, Lammi MJ, Uusitalo H, Viitanen M, Hyttinen MM, Jurvelin JS, Vuorio E, Helminen HJ, Tulamo RM.
(2004).
Effect of radiosynovectomy with holmium-166 ferric hydroxide macroaggregate on adult equine cartilage.
J Rheumatol, 31(2), 321-328.
Publication
Researcher Affiliations
- Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
MeSH Terms
- Animals
- Cartilage, Articular / metabolism
- Cartilage, Articular / pathology
- Female
- Glycosaminoglycans / metabolism
- Horses
- Hydroxides / therapeutic use
- Sulfates / pharmacokinetics
- Sulfur Radioisotopes
- Synovial Fluid / metabolism
- Synovial Membrane / metabolism
- Synovial Membrane / pathology
- Synovitis / pathology
- Synovitis / therapy
Citations
This article has been cited 3 times.- Kampen WU, Boddenberg-Pätzold B, Fischer M, Gabriel M, Klett R, Konijnenberg M, Kresnik E, Lellouche H, Paycha F, Terslev L, Turkmen C, van der Zant F, Antunovic L, Panagiotidis E, Gnanasegaran G, Kuwert T, Van den Wyngaert T. The EANM guideline for radiosynoviorthesis. Eur J Nucl Med Mol Imaging 2022 Jan;49(2):681-708.
- Klaassen NJM, Arntz MJ, Gil Arranja A, Roosen J, Nijsen JFW. The various therapeutic applications of the medical isotope holmium-166: a narrative review. EJNMMI Radiopharm Chem 2019 Aug 5;4(1):19.
- Linke R, Gelse K, Schuch F. [The indication for radiosynoviorthesis. From the perspective of the nuclear medicine expert, rheumatic orthopedist and internist]. Z Rheumatol 2011 Jan;70(1):34-44.
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