Effect of short-term hyperinsulinemia on the localization and expression of endothelin receptors A and B in lamellar tissue of the forelimbs of horses.
Abstract: To determine the effect of short-term hyperinsulinemia on the localization and expression of endothelin receptor (ETR)-A and ETR-B in lamellar tissue of the forelimbs of horses. Methods: Distal portion of 15 cadaveric forelimbs from healthy adult horses (1 limb/horse) obtained immediately after slaughter at an abattoir. Methods: Each forelimb was assigned to 1 of 3 treatment groups (perfused with autologous blood for 10 hours [control perfusion; n = 5], perfused with an insulin [142 ± 81 μU/mL] perfusate for 10 hours [insulinemic perfusion; 5], or not perfused [unperfused control; 5]). Immunohistochemical evaluation of lamellar tissue was performed to assess localization of ETR-A and ETR-B. Expression of ETR-A and ETR-B was measured semiquantitatively on a scale of 0 to 3 (0 = none, 1 = mild, 2 = moderate, and 3 = high-intensity staining) and quantitatively by means of gray value analysis with imaging software. Results: In all specimens, ETR-A and ETR-B were localized in endothelium, smooth muscle cells, axons, and keratinocytes. Quantitative expression of ETR-A in the midportion of the primary epidermal lamellae for the insulinemic perfusion group (149 ± 16) was lower than that for the control perfusion group (158 ± 15). Expression of ETR-B in the primary epidermal lamellae tips for the insulinemic perfusion group (140 ± 29) was higher than that for the control perfusion group (114 ± 8). Conclusions: Hyperinsulinemia caused significant changes in endothelin receptor expression, which suggested that ETR antagonists might be beneficial for treatment of laminitis in horses.
Publication Date: 2014-03-29 PubMed ID: 24669922PubMed Central: PMC4018679DOI: 10.2460/ajvr.75.4.367Google Scholar: Lookup
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- Journal Article
Summary
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The research article discusses the impact of short-term high insulin levels on the expression and location of endothelin receptors A and B in the lamellar tissue of horses’ forelimbs, with the prospect of treating laminitis in horses.
Study Methodology
- The study was conducted on the distal part of 15 cadaveric forelimbs of healthy adult horses. These were obtained immediately after the animals were slaughtered at an abattoir.
- The forelimbs were divided into three different treatment groups. These included a control group that was perfused with autologous blood for 10 hours, a group perfused with an insulin perfusate for the same duration, and an unperfused control group.
- The researchers assessed the localisation of ETR-A and ETR-B in the lamellar tissue through an immunohistochemical evaluation.
- The expression levels of ETR-A and ETR-B were measured both semi-quantitatively and quantitatively. A 0 to 3 scale was used for semi-quantitative measurement (with 0 meaning none, 1 meaning mild, 2 meaning moderate, and 3 meaning high-intensity staining). The quantitative measurement was performed using gray value analysis with imaging software.
Study Results
- The results showed that both ETR-A and ETR-B were localised in the endothelium, smooth muscle cells, axons, and keratinocytes in all study samples.
- The study found that the expression levels of ETR-A in the midportion of the primary epidermal lamellae in the insulinemic perfusion group were lower compared to the control perfusion group.
- On the flip side, ETR-B expression in the primary epidermal lamellae tips was higher in the insulinemic perfusion group compared to the control perfusion group.
Conclusions and Implications
- The study concluded that high insulin levels cause significant changes in the expression of endothelin receptors.
- This suggested potential benefits of endothelin receptor antagonists as a treatment measure for laminitis in horses. The finding is crucial as it opens up a new therapeutic approach to treat a debilitating condition that significantly affects the equine population.
Cite This Article
APA
Gauff FC, Patan-Zugaj B, Licka TF.
(2014).
Effect of short-term hyperinsulinemia on the localization and expression of endothelin receptors A and B in lamellar tissue of the forelimbs of horses.
Am J Vet Res, 75(4), 367-374.
https://doi.org/10.2460/ajvr.75.4.367 Publication
Researcher Affiliations
- Department of Horses and Small Animals, University of Veterinary Medicine Vienna, A-1210 Vienna, Austria.
MeSH Terms
- Animals
- Cadaver
- Forelimb
- Gene Expression Regulation
- Hoof and Claw / drug effects
- Hoof and Claw / metabolism
- Horses
- Hyperinsulinism / chemically induced
- Hyperinsulinism / veterinary
- Insulin / toxicity
- Perfusion
- Receptor, Endothelin A / genetics
- Receptor, Endothelin A / metabolism
- Receptor, Endothelin B / genetics
- Receptor, Endothelin B / metabolism
Grant Funding
- P 22598 / Austrian Science Fund FWF
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