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Home / Equine Research Bank / Equine Vet J / Effect of stabling on bronchoalveolar cells obtained from no...
Equine veterinary journal1993; 25(3); 194-197; doi: 10.1111/j.2042-3306.1993.tb02941.x

Effect of stabling on bronchoalveolar cells obtained from normal and COPD horses.

Abstract: Bronchoalveolar lavages (BAL) were performed before and after 3 weeks of housing in 5 horses suffering from COPD and 5 normal horses. In the two groups, the total number of cells recovered remained unchanged after stabling. The most common cell populations in BAL fluid of control animals were alveolar macrophages (46.4%) and lymphocytes (44.9%). The percentage of neutrophils increased after stabling from 8.7% to 27.6%. In COPD horses, lymphocytes predominated (40.7%) in animals at pasture with neutrophils increasing from 29.4% to 71.6% after stabling. After fractionation by Percoll density gradient, alveolar macrophages and neutrophils from normal and COPD horses had a similar density distribution. After stabling, these cells from normal horses were increased in the low density layers, while those from COPD horses were predominantly in the hyperdense layers. Therefore, BAL cells obtained from COPD animals at pasture and after stabling differ from those of control horses in the same environment, not only in their populations but also in their buoyant densities. These differences could be related to different states of cellular activation and perhaps be responsible for disease activity in the COPD horses.
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Publication Date: 1993-05-01 PubMed ID: 8508745DOI: 10.1111/j.2042-3306.1993.tb02941.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the effect of enclosing horses in stables on the cells present in the lungs, particularly focusing on those affected by chronic obstructive pulmonary disease (COPD). It found differences in cell populations and densities between healthy horses and those with COPD before and after they were stabled.

Research Methodology

  • The researchers performed bronchoalveolar lavages (BAL) – a procedure used to examine the cells in the lungs – on 5 horses suffering from COPD and 5 healthy horses.
  • The BALs were conducted before and after the horses were stabled for 3 weeks.
  • In examining the cells obtained through BAL, researchers noted the percentages and types of cells present as well as their densities after fractionation with Percoll density gradient.

Findings

  • In both COPD and healthy horses, the total number of cells recovered from the lungs didn’t change after being stabled.
  • However, the composition of these cells did alter. In healthy horses, alveolar macrophages and lymphocytes were the most common cells, comprising 46.4% and 44.9% respectively of the total cells. After stabling, the percentage of a certain type of white blood cell, neutrophils, rose from 8.7% to 27.6%.
  • In horses suffering from COPD, lymphocytes were the dominant cells when the horses were in the pasture, with neutrophils increasing from 29.4% to 71.6% after stabling.
  • Post-stabling, cells from healthy horses were found in greater numbers in the low-density layers post-fractionation, while cells from COPD horses were predominantly in the high-density layers.

Conclusions

  • The bronchoalveolar cells in COPD affected horses, both at pasture and following stabling, are not only different in their population but also in their densities when compared to healthy horses in the same environments.
  • The observed differences could potentially be due to different stages of cellular activation, and might be responsible for the disease activity observed in COPD horses.

Cite This Article

APA
Tremblay GM, Ferland C, Lapointe JM, Vrins A, Lavoie JP, Cormier Y. (1993). Effect of stabling on bronchoalveolar cells obtained from normal and COPD horses. Equine Vet J, 25(3), 194-197. https://doi.org/10.1111/j.2042-3306.1993.tb02941.x

Publication

Equine veterinary journal
ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 25
Issue: 3
Pages: 194-197

Researcher Affiliations

Tremblay, G M
  • Centre de pneumologie de l'Hôpital Laval, Université Laval, Sainte-Foy, Québec, Canada.
Ferland, C
    Lapointe, J M
      Vrins, A
        Lavoie, J P
          Cormier, Y

            MeSH Terms

            • Animals
            • Bronchoalveolar Lavage Fluid / pathology
            • Bronchoalveolar Lavage Fluid / veterinary
            • Cell Count / veterinary
            • Cell Separation / veterinary
            • Centrifugation, Density Gradient / veterinary
            • Female
            • Horse Diseases / pathology
            • Horses
            • Housing, Animal
            • Lung Diseases, Obstructive / pathology
            • Lung Diseases, Obstructive / veterinary
            • Macrophages, Alveolar
            • Male
            • Neutrophils

            Citations

            This article has been cited 16 times.
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