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Effect of staining and freezing media on sortability of stallion spermatozoa and their post-thaw viability after sex-sorting and cryopreservation.
Abstract: Sex-sorted, frozen-thawed stallion spermatozoa remain out of reach of commercial horse breeders because of the low efficiency of the sex-sorting process and unacceptable fertility rates after insemination. Two experiments were designed to test the effects of alternative staining and freezing media to improve the viability of sex-sorted frozen-thawed stallion spermatozoa. Experiment 1 compared two freezing media, INRA 82(®) and a modified lactose-ethylenediaminetetraacetic acid (EDTA), for the cryopreservation of sex-sorted stallion spermatozoa. No significant differences between the two freezing media could be identified, suggesting that both cryodiluents would be suitable for incorporation into a sex-preselection protocol for stallion spermatozoa. Experiment 2 compared Kenney's modified Tyrode's (KMT) and Sperm TALP (Sp-TALP) as the staining and incubation medium for stallion spermatozoa prior to sex-sorting. A significant increase in the percentage of acrosome-reacted spermatozoa occurred after staining and incubation in the clarified Sp-TALP compared with KMT. As no improvements in sorting rates were achieved using Sp-TALP, it was concluded that stallion sorting protocols could include KMT as the staining and incubation medium while either INRA 82(®) or lactose-EDTA could be employed as a cryodiluents.
© 2011 Blackwell Verlag GmbH.
Publication Date: 2011-11-07 PubMed ID: 22053724DOI: 10.1111/j.1439-0531.2007.01010.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates possible methods for improving the success rate for sex-sorting of stallion spermatozoa and their viability after being frozen and thawed. It compares different staining and freezing media to optimize the procedure for commercial use in horse breeding.
Experiment Design and Methods
- The researchers designed two experiments to test alternative staining and freezing media.
- The first experiment compared two freezing media, INRA 82(®) and a modified lactose-ethylenediaminetetraacetic acid (EDTA), for the cryopreservation of sex-sorted stallion spermatozoa.
- The second experiment compared Kenney’s modified Tyrode’s (KMT) and Sperm TALP (Sp-TALP) as the staining and incubation medium for stallion spermatozoa prior to sex-sorting.
Findings on Freezing Media
- The research found no significant difference between the effects of the two selected freezing media (INRA 82® and lactose-EDTA) on the viability of sex-sorted frozen-thawed stallion spermatozoa.
- The lack of difference suggests both of these cryodiluents could be effectively utilized in a sex-preselection protocol for spermatozoa.
Findings on Staining and Incubation Media
- When comparing the effects of Kenney’s modified Tyrode’s (KMT) and Sperm TALP (Sp-TALP) media on staining and incubating stallion spermatozoa before sex-sorting, a significant increase in the percentage of acrosome-reacted (an important part of the fertility process) spermatozoa was observed using Sp-TALP.
- However, the use of Sp-TALP did not improve sperm sorting rates. Thus, the researchers concluded that KMT could be included in stallion sorting protocols as a staining and incubation medium.
Conclusion
- The study is beneficial in proposing alternative staining and freezing media that may enhance the efficiency of stallion spermatozoa sex-sorting and cryopreservation for commercial horse breeding. Despite this, the absence of improvements in sperm sorting rates highlights that further research and experimentation in this area are required.
Cite This Article
APA
Clulow JR, Buss H, Evans G, Sieme H, Rath D, Morris LH, Maxwell WM.
(2011).
Effect of staining and freezing media on sortability of stallion spermatozoa and their post-thaw viability after sex-sorting and cryopreservation.
Reprod Domest Anim, 47(1), 1-7.
https://doi.org/10.1111/j.1439-0531.2007.01010.x Publication
Researcher Affiliations
- Faculty of Veterinary Science, University of Sydney, Sydney, Australia.
MeSH Terms
- Animals
- Cell Separation / veterinary
- Cell Survival
- Coloring Agents
- Cryopreservation / veterinary
- Cryoprotective Agents
- Female
- Horses
- Hot Temperature
- Male
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sex Preselection / methods
- Sex Preselection / veterinary
- Sperm Motility
- Spermatozoa / cytology
Citations
This article has been cited 1 times.- Yadav SK, Gangwar DK, Singh J, Tikadar CK, Khanna VV, Saini S, Dholpuria S, Palta P, Manik RS, Singh MK, Singla SK. An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm. Vet World 2017 May;10(5):498-504.
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