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Home / Equine Research Bank / Acta Vet Scand / Effect of timing of frozen semen insemination on pregnancy r...
Acta veterinaria Scandinavica1996; 37(3); 361-365; doi: 10.1186/BF03548102

Effect of timing of frozen semen insemination on pregnancy rate in mares.

Abstract: Thirty-four mares were inseminated with frozen semen from one stallion during 2 oestrous cycles, every 48 h until ovulation took place and within 12 h after ovulation. Semen was frozen using the Colorado method. The insemination dose was from 200 to 400 x 10(6) progressively motile spermatozoa. Ovaries were examined every 12 h to determine time of ovulation. Examination for pregnancy was carried out using ultrasonography, 15 days after ovulation. Thirty-five per cent of mares inseminated < 24 h and 23% of mares inseminated between 24-48 h before ovulation were pregnant (p = 0.388). The pregnancy rate in all mares inseminated before ovulation was 30%. In the mares inseminated within 12 h of ovulation, it was 18% (p = 0.253). Younger mares (aged 4-10 yr) had a higher pregnancy rate (59%) than older mares (aged 11-15 yr) (23%), but the difference was not statistically significant (p = 0.057). Trettiofyra ston inseminerades med fryst sperma från samma hingst under 2 brunstcykler; varannan dag före ovulationen och inom 12 timmar efter ovulationen. Sperman frystes enligt Colorado-metoden. Inseminationsdosen var 200–400 × 106 progressivt motila spermier. Äggstockarna undersöktes var 12:e timme för att fastställa ovulationstidpunkten. Dräktighetsundersökning utfördes med ultraljud 15 dagar efter ovulationen. Trettiofem procent av de ston som inseminerats < 24 h och 23% av de ston som inseminerats 24-48 h före ovulationen var dräktiga (p = 0.388). Dräktighetsprocenten hos samtliga ston som inseminerats före ovulationen var 30% och hos ston som inseminerats inom 12 timmar efter ovulationen 18% (p = 0.253). Yngre ston (4–10 år) hade en högre dräktighetsprocent (59%) än äldre ston (11–15 år) (23%), skillnaden var dock inte statistiskt signifikant (p = 0.057).
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Publication Date: 1996-01-01 PubMed ID: 8996881PubMed Central: PMC8063980DOI: 10.1186/BF03548102Google Scholar: Lookup
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Summary

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The research analyzed the impact of the timing of using frozen semen for insemination on the pregnancy rates in mares. It concluded that insemination done less than 24 hrs before ovulation yielded higher pregnancy rates and younger mares showed a higher probability of pregnancy compared to older ones.

Methodology

  • 34 mares were used for the study. They were inseminated with frozen semen from a single stallion in two estrous cycles. The insemination was timed every 48 hours until ovulation occured and within 12 hours post ovulation.
  • The frozen semen used was preserved using the Colorado method, a popular semen freezing technique that is known for ensuring the quality and longevity of sperm quality.
  • The dose for insemination varied between 200 to 400 x 10^6 progressively moving spermatozoa.
  • The ovaries of the mares were examined every 12 hours to ascertain the exact time of ovulation.

Observation and Results

  • Pregnancy tests on the mares were conducted using ultrasonography 15 days after ovulation.
  • The pregnancy rate among mares that were inseminated less than 24 hours to ovulation was 35% while that of mares inseminated between 24-48 hours before ovulation was 23%. This difference, however, was not statistically significant (p = 0.388).
  • The overall pregnancy in mares inseminated before ovulation occurrence was 30%.
  • The mares that were inseminated within 12 hours post ovulation had a comparatively lower pregnancy rate of 18% (p = 0.253).

Comparison Between Younger and Older Mares

  • The study also observed that the pregnancy rate varied with the age of the mares. Younger mares (aged between 4 to 10 years) showed a significantly higher pregnancy rate of 59% as compared to older mares (aged between 11 to 15 years) which had a pregnancy rate of 23%. Despite this significant difference, the finding was not statistically significant (p = 0.057).

Cite This Article

APA
Katila T, Celebi M, Koskinen E. (1996). Effect of timing of frozen semen insemination on pregnancy rate in mares. Acta Vet Scand, 37(3), 361-365. https://doi.org/10.1186/BF03548102

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 37
Issue: 3
Pages: 361-365

Researcher Affiliations

Katila, T
  • Faculty of Veterinary Medicine, University of Helsinki, Saari, Finland. Terttn.Katila@Helsinki.Fi
Celebi, M
    Koskinen, E

      MeSH Terms

      • Animals
      • Cryopreservation / veterinary
      • Estrus
      • Female
      • Horses
      • Insemination, Artificial / veterinary
      • Male
      • Ovulation
      • Pregnancy
      • Pregnancy Outcome / veterinary
      • Pregnancy, Animal
      • Semen Preservation / veterinary
      • Sperm Motility
      • Time Factors

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