Effect of uterine ozone therapy and anticoagulant sampling on oxidative stress parameters in mares.

The study investigates the impact of uterine ozone therapy and different anticoagulant sampling on oxidative stress parameters in mares.

Research Methodology

• Ten mares underwent uterine ozone therapy for three consecutive days. This treatment involved flushing the uterus with ozonated lactated Ringer’s solution and then insufflation with ozone‑oxygen gas.
• Serum samples were collected at various intervals – baseline, and days 3, 6, 10 and 17, to monitor the effect of the therapy on oxidative stress (OS) markers.
• To analyze the effect of various anticoagulants on OS parameters, plasma was obtained at baseline and again after six days of therapy. The anticoagulants used in this step were citrate, ethylenediaminetetraacetic acid (EDTA), and heparin.
• The contents measured included antioxidants like albumin and uric acid, total antioxidant capacity (TAC) using four different methods, total oxidant capacity (TOC), and lipid peroxidation. These were determined using a method known as photocolorimetry.
• Two types of statistical analyses were conducted – repeated measures ANOVA followed by Dunnett’s test, and Friedman followed by Dunn’s test.

Major Findings

• The uterine ozone therapy led to a significant reduction in the levels of uric acid and TAC in all four measured methods. At the same time, there was an increase in TOC on the third and sixth day following the therapy.
• No changes were observed in the levels of albumin and lipid peroxidation.
• The choice of anticoagulant sampling was found to have an impact on the detection of oxidative stress induced by the therapy, with some anticoagulants preventing the detection depending on the method of analysis used.

Conclusion

• The study concludes that uterine ozone therapy causes systemic oxidative stress in mares. Moreover, the choice of anticoagulant used during sampling can interfere with the tests carried out to measure oxidative stress.