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Home / Equine Research Bank / Vet Surg / Effects of a 10% dimethyl sulfoxide solution on radiocarpal ...
Veterinary surgery : VS2023; doi: 10.1111/vsu.13973

Effects of a 10% dimethyl sulfoxide solution on radiocarpal joint amikacin pharmacokinetics during intravenous regional limb perfusion in standing sedated horses.

Abstract: To determine the effect of a 10% dimethyl sulfoxide (DMSO) solution on the peak concentration (C ) of amikacin in the radiocarpal joint (RCJ) during intravenous regional limb perfusion (IVRLP) compared with 0.9% NaCl. Methods: Randomized crossover study. Methods: Seven healthy adult horses. Methods: The horses underwent IVRLP with 2 g of amikacin sulfate diluted to 60 mL using a 10% DMSO or 0.9% NaCl solution. Synovial fluid was collected from the RCJ at 5, 10, 15, 20, 25, and 30 minutes after IVRLP. The wide rubber tourniquet placed on the antebrachium was removed after the 30 min sample. Amikacin concentrations were quantified by a fluorescence polarization immunoassay. The mean C and time to peak concentration (T ) of amikacin within the RCJ were determined. A one-sided paired t-test was used to determine the differences between treatments. The significance level was p < .05. Results: The mean ± SD C in the DMSO group was 1361.8 ± 593 μg/mL and in the 0.9% NaCl group it was 860 ± 481.6 μg/mL (p = .058). Mean T using the 10% DMSO solution was 23 and 18 min using the 0.9% NaCl perfusate (p = .161). No adverse effects were associated with use of the 10% DMSO solution. Conclusions: Although there were higher mean peak synovial concentrations using the 10% DMSO solution no difference in synovial amikacin C between perfusate type was detected (p = .058). Conclusions: Use of a 10% DMSO solution in conjunction with amikacin during IVRLP is a feasible technique and does not negatively affect the synovial amikacin levels achieved. Further research is warranted to determine other effects of using DMSO during IVRLP.
© 2023 American College of Veterinary Surgeons.
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Publication Date: 2023-06-06 PubMed ID: 37280731DOI: 10.1111/vsu.13973Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated the impact of a 10% dimethyl sulfoxide (DMSO) or 0.9% NaCl solution on the top concentration of amikacin, an antibiotic, in the radiocarpal joint of horses during intravenous regional limb perfusion (IVRLP). The findings suggested that while using DMSO solution showed higher mean peak amikacin concentrations than the saline solution, there was no significant difference between the two types.

Study Design

  • The research was designed as a randomized crossover study, involving seven healthy adult horses.
  • Each horse underwent IVRLP with 2g of amikacin sulfate diluted to 60 mL using either a 10% DMSO soluton or 0.9% NaCl (saline) solution. IVRLP is a technique used in equine medicine to deliver high concentrations of antibiotics to specific areas of the limb.
  • A rubber tourniquet was situated on the antebrachium (forearm part of the horse’s limb)
  • and was removed after the 30 minutes sample.

Data Collection

  • Samples of synovial fluid (fluid that reduces friction between the articular cartilage of synovial joints during movement) were collected from the horses’ radiocarpal joints at 5-minute intervals for 30 minutes after the IVRLP procedure.
  • These samples were then subjected to a fluorescence polarization immunoassay, a method used in biochemistry to measure the concentration of a substance in a fluid, in this case amikacin, an antibiotic used in veterinary medicine.

Results and Conclusion

  • The peak concentration (C) for amikacin within the radiocarpal joint in the DMSO group stood at 1361.8 ± 593 μg/mL while it was 860 ± 481.6 μg/mL in the saline group. Although the concentration was higher with the DMSO solution, the data analysis failed to highlight a statistically significant difference (p= .058).
  • The average time taken to reach the peak concentration for the DMSO solution was 23 minutes and 18 minutes for the saline solution. However, this difference wasn’t statistically significant either (p= .161).
  • The study noted no adverse effects on the horses with the usage of the 10% DMSO solution.
  • Even though higher mean peak synovial amikacin concentrations were found when using the DMSO solution, the difference in synovial amikacin concentration between the two types of perfusate was not statistically significant.
  • The conclusion of the study was that using a 10% DMSO solution during IVRLP is a feasible technique that doesn’t negatively affect the concentration of synovial amikacin achieved. Further research was advocated to study other effects of DMSO usage during IVRLP.

Cite This Article

APA
Kilcoyne I, Nieto J, Magdesian KG, Nottle BF. (2023). Effects of a 10% dimethyl sulfoxide solution on radiocarpal joint amikacin pharmacokinetics during intravenous regional limb perfusion in standing sedated horses. Vet Surg. https://doi.org/10.1111/vsu.13973

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English

Researcher Affiliations

Kilcoyne, Isabelle
  • Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Los Angeles, California, USA.
Nieto, Jorge
  • Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Los Angeles, California, USA.
Magdesian, K Gary
  • Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Los Angeles, California, USA.
Nottle, Bridget F
  • Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.

Grant Funding

  • A22-0322 / American Quarter Horse Foundation
  • American Quarter Horse Foundation.

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