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Antioxidants (Basel, Switzerland)2020; 9(5); 444; doi: 10.3390/antiox9050444

Effects of Advanced Age, Pituitary Pars Intermedia Dysfunction and Insulin Dysregulation on Serum Antioxidant Markers in Horses.

Abstract: The study aims to assess the impact of age, pituitary pars intermedia dysfunction (PPID) and insulin dysregulation (ID) in horses on selected oxidative stress markers. The study includes 32 horses, divided into three groups: "young" adult group (aged 8-16 years old) "geriatric" group (aged 18-24 years old) and the "PPID" group (aged 15-31 years old). The PPID group was further divided into two subgroups: PPID ID+ and PPID ID- based on presence or absence of ID. We measured serum antioxidant stress markers in all horses: total oxidant status (TOS), total antioxidant capacity (TAC), ceruloplasmin (CER), lipofuscin (LPS), malondialdehyde (MDA) and thiols concentrations (containing sulfhydryl group -SH) as well as enzymatic systems: total superoxide dismutase (SOD), cytoplasmic SOD (CuZnSOD), mitochondrial SOD activity (MnSOD). Total serum thiols were significantly lower in the geriatric group and in the PPID group compared to the young group. The MnSOD concentration was higher in the PPID ID+ group compared to the PPID ID-. LPS and MDA concentrations were lower in the PPID ID+ group compared to the PPID ID- group. In the selected study groups of horses, older age, the presence of PPID and ID in the case of PPID had no effect on the studied oxidative stress markers.
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Publication Date: 2020-05-21 PubMed ID: 32455574PubMed Central: PMC7278619DOI: 10.3390/antiox9050444Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how age, pituitary dysfunctions, and insulin problems affect certain antioxidant markers in horses. It discovered lower levels of a particular type of antioxidant in older horses, and variations in antioxidant levels in horses with pituitary dysfunction based on their insulin health, but overall found no significant effect of these factors on the measured oxidative stress markers.

Research Overview

  • “Effects of Advanced Age, Pituitary Pars Intermedia Dysfunction and Insulin Dysregulation on Serum Antioxidant Markers in Horses” is a study assessing the impact of aging, pituitary pars intermedia dysfunction (PPID), and insulin dysregulation (ID) on oxidative stress markers in horses.
  • The research involved 32 horses divided into groups based on aging and medical conditions: a “young” adult group, a “geriatric” group, and a group of horses with PPID. The PPID group was further divided based on presence or absence of insulin dysregulation (ID+ and ID-).

Marker Assessment and Findings

  • All the participating horses underwent measurement of serum antioxidant stress markers including total oxidant status (TOS), total antioxidant capacity (TAC), ceruloplasmin (CER), lipofuscin (LPS), malondialdehyde (MDA), and sulfhydryl group-bearing thiols concentrations as well as enzymatic systems like total superoxide dismutase (SOD), cytoplasmic SOD (CuZnSOD), and mitochondrial SOD activity (MnSOD).
  • The total serum thiols levels were notably lower in both the geriatric group and the PPID group compared to the younger horse group.
  • Interestingly, the concentrations of MnSOD were higher in the PPID group with insulin dysregulation compared to the subgroup without insulin dysregulation. Similarly, LPS and MDA concentrations were lower in the ID+ subgroup.

Conclusion

  • The research concluded older age, the presence of PPID, and insulin dysregulation in horses with PPID did not significantly affect the oxidative stress markers studied.
  • This discovery could be beneficial for veterinary medicine, giving insights on how aging and certain dysfunctions impact oxidative stress in horses.

Cite This Article

APA
Żak A, Siwińska N, Chełmecka E, Bażanów B, Romuk E, Adams A, Niedźwiedź A, Stygar D. (2020). Effects of Advanced Age, Pituitary Pars Intermedia Dysfunction and Insulin Dysregulation on Serum Antioxidant Markers in Horses. Antioxidants (Basel), 9(5), 444. https://doi.org/10.3390/antiox9050444

Publication

Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
NlmUniqueID: 101668981
Country: Switzerland
Language: English
Volume: 9
Issue: 5
PII: 444

Researcher Affiliations

Żak, Agnieszka
  • Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland.
Siwińska, Natalia
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland.
Chełmecka, Elżbieta
  • Department of Statistics, Department of Instrumental Analysis, Faculty of Pharmaceutical Sciences in Sosnowiec Medical University of Silesia, 40-055 Katowice, Poland.
Bażanów, Barbara
  • Department of Pathology, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
Romuk, Ewa
  • Department of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Katowice, Poland.
Adams, Amanda
  • Department of Veterinary Science, MH Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
Niedźwiedź, Artur
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland.
Stygar, Dominika
  • Department of Physiology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, 41-808 Katowice, Poland.

Conflict of Interest Statement

The authors declare no conflict of interest.

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