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Home / Equine Research Bank / Front Vet Sci / Effects of Autologous Conditioned Serum, Autologous Protein ...
Frontiers in veterinary science2020; 7; 323; doi: 10.3389/fvets.2020.00323

Effects of Autologous Conditioned Serum, Autologous Protein Solution, and Triamcinolone on Inflammatory and Catabolic Gene Expression in Equine Cartilage and Synovial Explants Treated With IL-1β in Co-culture.

Abstract: Autologous conditioned serum (ACS) and autologous protein solution (APS) are newer therapeutic options for osteoarthritis (OA). Co-culture of cartilage and synovium stimulated with IL-1β produces a similar physiologic response to tissues from naturally-ocurring OA. The study objective was to investigate the effects of ACS, APS, and triamcinolone (TA) on inflammatory and catabolic gene expression of inflamed joint tissues in co-culture. Blood was collected and processed for ACS and APS from six horses. Cartilage and synovial explants were harvested from the stifle, placed in co-culture, and treated as: (1) unstimulated control (2) stimulated control (3) ACS at 25% v/v (4) ACS at 50% v/v (5) APS at 25% v/v (6) APS at 50% v/v, (7) TA (10 M). Treatment groups 2-7 were stimulated with IL-1β (10 ng/ml). Cultures were maintained for 96 hours, and then both media and explants were harvested for measurement of gene expression and protein. IL-1β stimulation significantly increased IL-1β ( = 0.029), IL-8 ( = 0.011) and MMP-3 ( = 0.043) expression in synovium and IL-1β ( = 0.003) and TNF-α ( = 0.001) expression in cartilage. Treatment with 50% ACS and APS v/v downregulated IL-1β expression in cartilage more than TA treatment ( = 0.001 and = 0.0004) and APS downregulated MMP-1 expression in synovial membrane ( = 0.025). Treatment with ACS and APS caused a trend in upregulation of IL-10 expression in synovium and type II collagen and aggrecan expression in cartilage. PGE media concentrations were significantly reduced following treatment with APS (13.7-fold decrease, = 0.0001) and ACS (4.13-fold decrease, = 0.024); while TA did not reduce PGE significantly (2.3-fold decreased = 0.406). As disease-modifying therapies, ACS and APS modified the cellular response from synovial membrane and articular cartilage. ACS and APS may offer an improved strategy to improve clinical signs of horses with naturally occurring OA, compared to TA treatment.
Copyright © 2020 Velloso Alvarez, Boone, Pondugula, Caldwell and Wooldridge.
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Publication Date: 2020-06-26 PubMed ID: 32671108PubMed Central: PMC7332692DOI: 10.3389/fvets.2020.00323Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated the potential therapeutic effects of two new methods, Autologous conditioned serum (ACS) and Autologous protein solution (APS), in treating osteoarthritis (OA) in horses. They were compared against Triamcinolone (TA), a current treatment option, to evaluate their influence on inflammation and tissue destruction in OA.

Research Procedure

  • Blood samples were collected from six horses to prepare ACS and APS. Cartilage and synovial tissues, which make up a major part of the joints and are affected in OA, were also harvested from the horses.
  • The collected tissues were arranged in a co-culture, or a combined culture, and different treatments were applied. These include ACS and APS at varying concentrations, TA, and an unstimulated control for comparison. These tissues were also stimulated using an inflammatory agent (IL-1β) to mimic the inflammatory conditions in naturally occurring OA.
  • The cultures were maintained for 96 hours. After this incubation period, the media and tissues were studied for changes in gene expression, the most crucial of which are involved in inflammation (IL-1β, TNF-α, IL-10) and tissue destruction (MMP-3, MMP-1).

Results

  • Stimulation with IL-1β significantly increased the expression of IL-1β, IL-8, MMP-3, and TNF-α, implying a successful recreation of OA conditions.
  • ACS and APS successfully reduced the expression of IL-1β (a crucial inflammation marker) in cartilage more than TA did. APS also brought down the levels of MMP-1 (a marker for tissue destruction) in the synovial membrane.
  • APS and ACS, to an extent, resulted in an upregulation of IL-10, type II collagen, and aggrecan, suggesting therapeutic activity.
  • The media concentrations of PGE, a significant player in inflammation, were lowered by APS and ACS significantly. This result demonstrates their potential in controlling inflammation in OA.

Conclusion

  • As per the results of this study, ACS and APS show promise as therapies for OA in horses by modifying the cellular response to inflammation and tissue destruction in the joint.
  • They could present a more impressive strategy for improving clinical symptoms of naturally occurring OA in horses than the conventional TA treatment.

Cite This Article

APA
Velloso Alvarez A, Boone LH, Pondugula SR, Caldwell F, Wooldridge AA. (2020). Effects of Autologous Conditioned Serum, Autologous Protein Solution, and Triamcinolone on Inflammatory and Catabolic Gene Expression in Equine Cartilage and Synovial Explants Treated With IL-1β in Co-culture. Front Vet Sci, 7, 323. https://doi.org/10.3389/fvets.2020.00323

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 7
Pages: 323
PII: 323

Researcher Affiliations

Velloso Alvarez, Ana
  • Department of Clinical Sciences, Auburn University, Auburn, AL, United States.
Boone, Lindsey H
  • Department of Clinical Sciences, Auburn University, Auburn, AL, United States.
Pondugula, Satyanarayana R
  • Department of Anatomy, Physiology and Pharmacology, Auburn University, Auburn, AL, United States.
Caldwell, Fred
  • Department of Clinical Sciences, Auburn University, Auburn, AL, United States.
Wooldridge, Anne A
  • Department of Clinical Sciences, Auburn University, Auburn, AL, United States.

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