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The American journal of sports medicine2006; 34(11); 1824-1831; doi: 10.1177/0363546506289882

Effects of calcified cartilage on healing of chondral defects treated with microfracture in horses.

Abstract: Microfracture of full-thickness articular defects has been shown to significantly enhance the amount of repair tissue. However, there is a suggestion that leaving calcified cartilage inhibits this repair response. Objective: Removal of the calcified cartilage with retention of subchondral bone enhances the amount of attachment of the repair tissue compared with retention of the calcified cartilage layer. Methods: Controlled laboratory study. Methods: There were 1-cm(2) articular cartilage defects made in 12 skeletally mature horses on the axial weightbearing portion of both medial femoral condyles. Using a custom measuring device and direct arthroscopic observation of the subchondral bone beneath the calcified cartilage layer, the authors removed the calcified cartilage from 1 defect of each horse. The repair was assessed with arthroscopy, clinical examination, radiographic and magnetic resonance imaging examinations, biopsy at 4 months, gross and histopathologic examinations at 12 months, as well as mRNA and immunohistochemical evaluations. Results: Removal of calcified cartilage with retention of the subchondral bone plate increased the overall repair tissue as assessed by arthroscopic (4 months) and gross evaluation (12 months). An increase in the level of the subchondral bone was also observed with removal of the calcified cartilage layer. The clinical pain, radiographic examinations, magnetic resonance imaging evaluations, histologic character, matrix proteins, or mRNA expression do not appear to differ based on level of defect debridement. Conclusions: Removal of the calcified cartilage layer appears to provide optimal amount and attachment of repair tissue. Therefore, close arthroscopic visualization is recommended for debridement of clinical lesions to ensure removal of the calcified cartilage layer.
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Publication Date: 2006-07-10 PubMed ID: 16832126DOI: 10.1177/0363546506289882Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the effects of calcified cartilage removal on the healing of full-thickness articular defects in horses. The findings show that removing the calcified cartilage layer enhances the repair tissue’s growth and attachment, without affecting other parameters like pain levels, matrix proteins, or gene expression.

Research Methodology

  • An experiment was conducted on 12 mature horses by artificially creating a 1-cm² articular cartilage defect on both medial femoral condyles, which is a commonly weight-bearing portion of the bone.
  • The calcified cartilage layer was removed from one defect in each horse, while leaving the subchondral bone intact.
  • The authors employed a custom measuring device and direct arthroscopic observation to accurately gauge the amount of subchondral bone beneath the removed cartilage layer.
  • The progress of the cartilage repair was monitored over a period of one year, using arthroscopy, clinical examinations, biopsies, radiographic and magnetic resonance imaging, as well as gross and histopathologic examinations.
  • Additional assessment methods included mRNA and immunohistochemical evaluations to scrutinize potential molecular changes.

Research Findings

  • The results consistently showed that removal of the calcified cartilage layer led to an increase in were the volume of repair tissue as per the evaluations conducted via arthroscopy (at 4 months) as well as gross examination (at 12 months).
  • There was also a noticeable increase in the level of the subchondral bone upon removing the calcified cartilage layer.
  • Notably, none of the other parameters such as clinical pain, radiographic evaluations, magnetic resonance imaging evaluations, histologic character, or even the expression levels of matrix proteins mRNA appeared to be affected by the level of defect debridement.

Conclusions

  • The findings strongly imply that removing the calcified cartilage layer enhances the extent and attachment of repair tissue, thereby potentially improving the healing process for full-thickness articular defects.
  • The authors recommend close arthroscopic visualization during debridement of clinical lesions in order to ensure adequate removal of the calcified cartilage layer, as it appears to provide the optimal condition for cartilage regeneration.

Cite This Article

APA
Frisbie DD, Morisset S, Ho CP, Rodkey WG, Steadman JR, McIlwraith CW. (2006). Effects of calcified cartilage on healing of chondral defects treated with microfracture in horses. Am J Sports Med, 34(11), 1824-1831. https://doi.org/10.1177/0363546506289882

Publication

The American journal of sports medicine
ISSN: 0363-5465
NlmUniqueID: 7609541
Country: United States
Language: English
Volume: 34
Issue: 11
Pages: 1824-1831

Researcher Affiliations

Frisbie, David D
  • Equine Orthopaedic Research Center, Colorado State University, 300 West Drake Road, Fort Collins, 80523, USA.
Morisset, Sophie
    Ho, Charles P
      Rodkey, William G
        Steadman, J Richard
          McIlwraith, C Wayne

            MeSH Terms

            • Animals
            • Calcinosis / pathology
            • Calcinosis / surgery
            • Cartilage Diseases / pathology
            • Cartilage Diseases / surgery
            • Cartilage, Articular / injuries
            • Cartilage, Articular / metabolism
            • Cartilage, Articular / pathology
            • Cartilage, Articular / surgery
            • Collagen / metabolism
            • Debridement
            • Femur / surgery
            • Horses
            • Insulin-Like Growth Factor I / metabolism
            • Matrix Metalloproteinase 3 / metabolism
            • Models, Animal
            • Orthopedic Procedures
            • Osteogenesis
            • RNA, Messenger / metabolism
            • Wound Healing

            Citations

            This article has been cited 63 times.
            1. Pohlig F, Wittek M, VON Thaden A, Lenze U, Glowalla C, Minzlaff P, Burgkart R, Prodinger PM. Biomechanical Properties of Repair Cartilage Tissue Are Superior Following Microdrilling Compared to Microfracturing in Critical Size Cartilage Defects. In Vivo 2023 Mar-Apr;37(2):565-573.
              doi: 10.21873/invivo.13115pubmed: 36881065google scholar: lookup
            2. Yu L, Cavelier S, Hannon B, Wei M. Recent development in multizonal scaffolds for osteochondral regeneration. Bioact Mater 2023 Jul;25:122-159.
              doi: 10.1016/j.bioactmat.2023.01.012pubmed: 36817819google scholar: lookup
            3. Li M, Zhang L, Li J, Zhu Q. Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-like Cells. Biomedicines 2022 Oct 14;10(10).
              doi: 10.3390/biomedicines10102582pubmed: 36289842google scholar: lookup
            4. Nordberg RC, Otarola GA, Wang D, Hu JC, Athanasiou KA. Navigating regulatory pathways for translation of biologic cartilage repair products. Sci Transl Med 2022 Aug 24;14(659):eabp8163.
              doi: 10.1126/scitranslmed.abp8163pubmed: 36001677google scholar: lookup
            5. Swindell HW, Chen AZ, Anderson MJ, Tedesco LJ, McCormick KL, Popkin CA, Saltzman BM, Trofa DP. Return to Play Criteria Following Surgical Management of Osteochondral Defects of the Knee: A Systematic Review. Cartilage 2022 Jul-Sep;13(3):19476035221098164.
              doi: 10.1177/19476035221098164pubmed: 35819020google scholar: lookup
            6. Peng L, Zhang B, Luo X, Huang B, Zhou J, Jiang S, Guo W, Tian G, Tian Z, Shen S, Li Y, Sui X, Liu S, Guo Q, Li H. Small Ruminant Models for Articular Cartilage Regeneration by Scaffold-Based Tissue Engineering. Stem Cells Int 2021;2021:5590479.
              doi: 10.1155/2021/5590479pubmed: 34912460google scholar: lookup
            7. Ono Y, Akagi R, Mikami Y, Shinohara M, Hosokawa H, Horii M, Watanabe S, Ogawa Y, Sadamasu A, Kimura S, Yamaguchi S, Ohtori S, Sasho T. Effect of Systemic Administration of Granulocyte Colony-Stimulating Factor on a Chronic Partial-Thickness Cartilage Defect in a Rabbit Knee Joint. Cartilage 2021 Dec;13(2_suppl):175S-184S.
              doi: 10.1177/19476035211021905pubmed: 34105400google scholar: lookup
            8. Fikes KK, Coverdale JA, Leatherwood JL, Campbell JM, Welsh TH Jr, Hartz CJ, Goehring M, Millican AA, Bradbery AN, Wickersham TA. Effect of bioactive proteins on gait kinematics and systemic inflammatory markers in mature horses. Transl Anim Sci 2021 Jan;5(1):txab017.
              doi: 10.1093/tas/txab017pubmed: 33748684google scholar: lookup
            9. Monaco G, El Haj AJ, Alini M, Stoddart MJ. Ex Vivo Systems to Study Chondrogenic Differentiation and Cartilage Integration. J Funct Morphol Kinesiol 2021 Jan 5;6(1).
              doi: 10.3390/jfmk6010006pubmed: 33466400google scholar: lookup
            10. Gao L, Cucchiarini M, Madry H. Cyst formation in the subchondral bone following cartilage repair. Clin Transl Med 2020 Dec;10(8):e248.
              doi: 10.1002/ctm2.248pubmed: 33377663google scholar: lookup
            11. Shah SS, Mithoefer K. Current Applications of Growth Factors for Knee Cartilage Repair and Osteoarthritis Treatment. Curr Rev Musculoskelet Med 2020 Dec;13(6):641-650.
              doi: 10.1007/s12178-020-09664-6pubmed: 32710292google scholar: lookup
            12. Fugazzola MC, van Weeren PR. Surgical osteochondral defect repair in the horse-a matter of form or function?. Equine Vet J 2020 Jul;52(4):489-499.
              doi: 10.1111/evj.13231pubmed: 31958175google scholar: lookup
            13. Tomaszewski R, Wiktor Ł, Gap A. Enhancement of cartilage repair through the addition of growth plate chondrocytes in an immature skeleton animal model. J Orthop Surg Res 2019 Aug 15;14(1):260.
              doi: 10.1186/s13018-019-1302-ypubmed: 31416470google scholar: lookup
            14. Guo T, Noshin M, Baker HB, Taskoy E, Meredith SJ, Tang Q, Ringel JP, Lerman MJ, Chen Y, Packer JD, Fisher JP. 3D printed biofunctionalized scaffolds for microfracture repair of cartilage defects. Biomaterials 2018 Dec;185:219-231.
              doi: 10.1016/j.biomaterials.2018.09.022pubmed: 30248646google scholar: lookup
            15. Rauch A, Rembeck E, Kohn L. [Operative treatment of cartilage lesions]. Orthopade 2018 Nov;47(11):965-978.
              doi: 10.1007/s00132-018-3630-8pubmed: 30178116google scholar: lookup
            16. Nam Y, Rim YA, Lee J, Ju JH. Current Therapeutic Strategies for Stem Cell-Based Cartilage Regeneration. Stem Cells Int 2018;2018:8490489.
              doi: 10.1155/2018/8490489pubmed: 29765426google scholar: lookup
            17. Gao L, Goebel LKH, Orth P, Cucchiarini M, Madry H. Subchondral drilling for articular cartilage repair: a systematic review of translational research. Dis Model Mech 2018 Jun 19;11(6).
              doi: 10.1242/dmm.034280pubmed: 29728409google scholar: lookup
            18. El-Radi MA, Marin-Peña OR, Said HG, Tey-Pons M. Basics in hip chondrolabral lesions and state of the art. SICOT J 2017;3:73.
              doi: 10.1051/sicotj/2017040pubmed: 29309028google scholar: lookup
            19. Seow D, Yasui Y, Hutchinson ID, Hurley ET, Shimozono Y, Kennedy JG. The Subchondral Bone Is Affected by Bone Marrow Stimulation: A Systematic Review of Preclinical Animal Studies. Cartilage 2019 Jan;10(1):70-81.
              doi: 10.1177/1947603517711220pubmed: 28573889google scholar: lookup
            20. Gao L, Orth P, Müller-Brandt K, Goebel LK, Cucchiarini M, Madry H. Early loss of subchondral bone following microfracture is counteracted by bone marrow aspirate in a translational model of osteochondral repair. Sci Rep 2017 Mar 27;7:45189.
              doi: 10.1038/srep45189pubmed: 28345610google scholar: lookup
            21. Demange MK, Minas T, von Keudell A, Sodha S, Bryant T, Gomoll AH. Intralesional Osteophyte Regrowth Following Autologous Chondrocyte Implantation after Previous Treatment with Marrow Stimulation Technique. Cartilage 2017 Apr;8(2):131-138.
              doi: 10.1177/1947603516653208pubmed: 28345403google scholar: lookup
            22. Siriwanarangsun P, Statum S, Biswas R, Bae WC, Chung CB. Ultrashort time to echo magnetic resonance techniques for the musculoskeletal system. Quant Imaging Med Surg 2016 Dec;6(6):731-743.
              doi: 10.21037/qims.2016.12.06pubmed: 28090449google scholar: lookup
            23. Gao L, Orth P, Goebel LK, Cucchiarini M, Madry H. A novel algorithm for a precise analysis of subchondral bone alterations. Sci Rep 2016 Sep 6;6:32982.
              doi: 10.1038/srep32982pubmed: 27596562google scholar: lookup
            24. Green CJ, Beck A, Wood D, Zheng MH. The biology and clinical evidence of microfracture in hip preservation surgery. J Hip Preserv Surg 2016 Jul;3(2):108-23.
              doi: 10.1093/jhps/hnw007pubmed: 27583147google scholar: lookup
            25. Salzmann GM, Niemeyer P, Vogt S, Kreuz P, Arnold M, Fritz J, Mujeeb A, Rosenberger R, Steinwachs M, Angele P. Practical execution of defect preparation prior to surgical cartilage intervention: results from a representative meeting survey among experts. Springerplus 2015;4:682.
              doi: 10.1186/s40064-015-1451-3pubmed: 27385105google scholar: lookup
            26. Stubbs AJ, Howse EA, Mannava S. Tissue engineering and the future of hip cartilage, labrum and ligamentum teres. J Hip Preserv Surg 2016 Apr;3(1):23-9.
              doi: 10.1093/jhps/hnv051pubmed: 27026815google scholar: lookup
            27. Madry H, Ochi M, Cucchiarini M, Pape D, Seil R. Large animal models in experimental knee sports surgery: focus on clinical translation. J Exp Orthop 2015 Dec;2(1):9.
              doi: 10.1186/s40634-015-0025-1pubmed: 26914877google scholar: lookup
            28. Meng Q, Man Z, Dai L, Huang H, Zhang X, Hu X, Shao Z, Zhu J, Zhang J, Fu X, Duan X, Ao Y. A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration. Sci Rep 2015 Dec 3;5:17802.
              doi: 10.1038/srep17802pubmed: 26632447google scholar: lookup
            29. Harris JD, Erickson BJ, Cvetanovich GL, Abrams GD, McCormick FM, Gupta AK, Verma NN, Bach BR Jr, Cole BJ. Development of a Valid and Reliable Knee Articular Cartilage Condition-Specific Study Methodological Quality Score. Orthop J Sports Med 2014 Feb;2(2):2325967113512606.
              doi: 10.1177/2325967113512606pubmed: 26535295google scholar: lookup
            30. Shive MS, Stanish WD, McCormack R, Forriol F, Mohtadi N, Pelet S, Desnoyers J, Méthot S, Vehik K, Restrepo A. BST-CarGel® Treatment Maintains Cartilage Repair Superiority over Microfracture at 5 Years in a Multicenter Randomized Controlled Trial. Cartilage 2015 Apr;6(2):62-72.
              doi: 10.1177/1947603514562064pubmed: 26069709google scholar: lookup
            31. Bell AD, Lascau-Coman V, Sun J, Chen G, Lowerison MW, Hurtig MB, Hoemann CD. Bone-Induced Chondroinduction in Sheep Jamshidi Biopsy Defects with and without Treatment by Subchondral Chitosan-Blood Implant: 1-Day, 3-Week, and 3-Month Repair. Cartilage 2013 Apr;4(2):131-43.
              doi: 10.1177/1947603512463227pubmed: 26069656google scholar: lookup
            32. Mithoefer K, Steadman RJ. Microfracture in Football (Soccer) Players: A Case Series of Professional Athletes and Systematic Review. Cartilage 2012 Jan;3(1 Suppl):18S-24S.
              doi: 10.1177/1947603511418960pubmed: 26069602google scholar: lookup
            33. McIlwraith CW, Fortier LA, Frisbie DD, Nixon AJ. Equine Models of Articular Cartilage Repair. Cartilage 2011 Oct;2(4):317-26.
              doi: 10.1177/1947603511406531pubmed: 26069590google scholar: lookup
            34. Hoemann C, Kandel R, Roberts S, Saris DB, Creemers L, Mainil-Varlet P, Méthot S, Hollander AP, Buschmann MD. International Cartilage Repair Society (ICRS) Recommended Guidelines for Histological Endpoints for Cartilage Repair Studies in Animal Models and Clinical Trials. Cartilage 2011 Apr;2(2):153-72.
              doi: 10.1177/1947603510397535pubmed: 26069577google scholar: lookup
            35. Hurtig MB, Buschmann MD, Fortier LA, Hoemann CD, Hunziker EB, Jurvelin JS, Mainil-Varlet P, McIlwraith CW, Sah RL, Whiteside RA. Preclinical Studies for Cartilage Repair: Recommendations from the International Cartilage Repair Society. Cartilage 2011 Apr;2(2):137-52.
              doi: 10.1177/1947603511401905pubmed: 26069576google scholar: lookup
            36. McAdams TR, Mithoefer K, Scopp JM, Mandelbaum BR. Articular Cartilage Injury in Athletes. Cartilage 2010 Jul;1(3):165-79.
              doi: 10.1177/1947603509360210pubmed: 26069548google scholar: lookup
            37. Salata MJ, Kercher JS, Bajaj S, Verma NN, Cole BJ. Glenohumeral Microfracture. Cartilage 2010 Apr;1(2):121-6.
              doi: 10.1177/1947603510366577pubmed: 26069543google scholar: lookup
            38. McIlwraith CW, Frisbie DD. Microfracture: Basic Science Studies in the Horse. Cartilage 2010 Apr;1(2):87-95.
              doi: 10.1177/1947603510367427pubmed: 26069539google scholar: lookup
            39. Steadman JR, Rodkey WG, Briggs KK. Microfracture: Its History and Experience of the Developing Surgeon. Cartilage 2010 Apr;1(2):78-86.
              doi: 10.1177/1947603510365533pubmed: 26069538google scholar: lookup
            40. Goebel L, Müller A, Bücker A, Madry H. High resolution MRI imaging at 9.4 Tesla of the osteochondral unit in a translational model of articular cartilage repair. BMC Musculoskelet Disord 2015 Apr 16;16:91.
              doi: 10.1186/s12891-015-0543-0pubmed: 25888208google scholar: lookup
            41. Van Dyck P, Vanhevel F, Vanhoenacker FM, Wouters K, Grodzki DM, Gielen JL, Parizel PM. Morphological MR imaging of the articular cartilage of the knee at 3 T-comparison of standard and novel 3D sequences. Insights Imaging 2015 Jun;6(3):285-93.
              doi: 10.1007/s13244-015-0405-1pubmed: 25855564google scholar: lookup
            42. Yanke AB, Chubinskaya S. The state of cartilage regeneration: current and future technologies. Curr Rev Musculoskelet Med 2015 Mar;8(1):1-8.
              doi: 10.1007/s12178-014-9254-7pubmed: 25591739google scholar: lookup
            43. Miller RE, Grodzinsky AJ, Barrett MF, Hung HH, Frank EH, Werpy NM, McIlwraith CW, Frisbie DD. Effects of the combination of microfracture and self-assembling Peptide filling on the repair of a clinically relevant trochlear defect in an equine model. J Bone Joint Surg Am 2014 Oct 1;96(19):1601-9.
              doi: 10.2106/JBJS.M.01408pubmed: 25274785google scholar: lookup
            44. Martins EA, Michelacci YM, Baccarin RY, Cogliati B, Silva LC. Evaluation of chitosan-GP hydrogel biocompatibility in osteochondral defects: an experimental approach. BMC Vet Res 2014 Aug 27;10:197.
              doi: 10.1186/s12917-014-0197-4pubmed: 25160583google scholar: lookup
            45. Bae WC, Biswas R, Chen K, Chang EY, Chung CB. UTE MRI of the Osteochondral Junction. Curr Radiol Rep 2014 Feb 1;2(2):35.
              doi: 10.1007/s40134-013-0035-7pubmed: 25061547google scholar: lookup
            46. Baums MH, Schultz W, Kostuj T, Klinger HM. Cartilage repair techniques of the talus: An update. World J Orthop 2014 Jul 18;5(3):171-9.
              doi: 10.5312/wjo.v5.i3.171pubmed: 25035819google scholar: lookup
            47. Gigliuto C, De Gregori M, Malafoglia V, Raffaeli W, Compagnone C, Visai L, Petrini P, Avanzini MA, Muscoli C, Viganò J, Calabrese F, Dominioni T, Allegri M, Cobianchi L. Pain assessment in animal models: do we need further studies?. J Pain Res 2014;7:227-36.
              doi: 10.2147/JPR.S59161pubmed: 24855386google scholar: lookup
            48. Park HW, Lee KB. Comparison of chondral versus osteochondral lesions of the talus after arthroscopic microfracture. Knee Surg Sports Traumatol Arthrosc 2015 Mar;23(3):860-7.
              doi: 10.1007/s00167-014-3061-ypubmed: 24841942google scholar: lookup
            49. de Vries-van Melle ML, Narcisi R, Kops N, Koevoet WJ, Bos PK, Murphy JM, Verhaar JA, van der Kraan PM, van Osch GJ. Chondrogenesis of mesenchymal stem cells in an osteochondral environment is mediated by the subchondral bone. Tissue Eng Part A 2014 Jan;20(1-2):23-33.
              doi: 10.1089/ten.TEA.2013.0080pubmed: 23980750google scholar: lookup
            50. Benthien JP, Behrens P. Reviewing subchondral cartilage surgery: considerations for standardised and outcome predictable cartilage remodelling: a technical note. Int Orthop 2013 Nov;37(11):2139-45.
              doi: 10.1007/s00264-013-2025-zpubmed: 23917852google scholar: lookup
            51. McIlwraith CW, Frisbie DD, Kawcak CE. The horse as a model of naturally occurring osteoarthritis. Bone Joint Res 2012 Nov;1(11):297-309.
              doi: 10.1302/2046-3758.111.2000132pubmed: 23610661google scholar: lookup
            52. Seo SS, Kim CW, Jung DW. Management of focal chondral lesion in the knee joint. Knee Surg Relat Res 2011 Dec;23(4):185-96.
              doi: 10.5792/ksrr.2011.23.4.185pubmed: 22570833google scholar: lookup
            53. Theodoropoulos J, Dwyer T, Whelan D, Marks P, Hurtig M, Sharma P. Microfracture for knee chondral defects: a survey of surgical practice among Canadian orthopedic surgeons. Knee Surg Sports Traumatol Arthrosc 2012 Dec;20(12):2430-7.
              doi: 10.1007/s00167-012-1925-6pubmed: 22362098google scholar: lookup
            54. Gomoll AH, Filardo G, de Girolamo L, Espregueira-Mendes J, Marcacci M, Rodkey WG, Steadman JR, Zaffagnini S, Kon E. Surgical treatment for early osteoarthritis. Part I: cartilage repair procedures. Knee Surg Sports Traumatol Arthrosc 2012 Mar;20(3):450-66.
              doi: 10.1007/s00167-011-1780-xpubmed: 22113219google scholar: lookup
            55. Heir S, Årøen A, Løken S, Holme I, Engebretsen L, Reinholt FP. Cartilage repair in the rabbit knee: mosaic plasty resulted in higher degree of tissue filling but affected subchondral bone more than microfracture technique: a blinded, randomized, controlled, long-term follow-up trial in 88 knees. Knee Surg Sports Traumatol Arthrosc 2012 Feb;20(2):197-209.
              doi: 10.1007/s00167-011-1596-8pubmed: 21892626google scholar: lookup
            56. de Vries-van Melle ML, Mandl EW, Kops N, Koevoet WJ, Verhaar JA, van Osch GJ. An osteochondral culture model to study mechanisms involved in articular cartilage repair. Tissue Eng Part C Methods 2012 Jan;18(1):45-53.
              doi: 10.1089/ten.TEC.2011.0339pubmed: 21875392google scholar: lookup
            57. Choi JA, Gold GE. MR imaging of articular cartilage physiology. Magn Reson Imaging Clin N Am 2011 May;19(2):249-82.
              doi: 10.1016/j.mric.2011.02.010pubmed: 21665090google scholar: lookup
            58. Bae WC, Dwek JR, Znamirowski R, Statum SM, Hermida JC, D'Lima DD, Sah RL, Du J, Chung CB. Ultrashort echo time MR imaging of osteochondral junction of the knee at 3 T: identification of anatomic structures contributing to signal intensity. Radiology 2010 Mar;254(3):837-45.
              doi: 10.1148/radiol.09081743pubmed: 20177096google scholar: lookup
            59. Byun J, Jung M, Chung K, Moon HS, Jung SH, Kim JK, Kim SH. Comparison of Magnetic Resonance Observation of Cartilage Repair Tissue Score and Functional Outcomes between Microdrilling and Microfracture for Cartilage Lesions of Distal Femur: A Retrospective Comparative Study. Cartilage 2025 Jul 29;:19476035251360504.
              doi: 10.1177/19476035251360504pubmed: 40726403google scholar: lookup
            60. Md Yusoff BAH, Hazli MI, Mohamad N, Ramlan MKR, Kamrulzaman NKABN, Rameez MR, Aziz MA. Clinical outcomes of a novel single-stage cartilage repair technique using calcified cartilage zone debridement with hyalofast. PLoS One 2025;20(7):e0328978.
              doi: 10.1371/journal.pone.0328978pubmed: 40705782google scholar: lookup
            61. van der Weiden GS, Mastbergen SC, Both SK, Karperien M, Lafeber FP, van Egmond N, Custers RJH. Dextran-tryamine hydrogel maintains position and integrity under simulated loading in a human cadaver knee model. Osteoarthr Cartil Open 2024 Sep;6(3):100492.
              doi: 10.1016/j.ocarto.2024.100492pubmed: 38946794google scholar: lookup
            62. Cognetti DJ, Defoor MT, Yuan TT, Sheean AJ. Knee Joint Preservation in Tactical Athletes: A Comprehensive Approach Based upon Lesion Location and Restoration of the Osteochondral Unit. Bioengineering (Basel) 2024 Mar 1;11(3).
              doi: 10.3390/bioengineering11030246pubmed: 38534520google scholar: lookup
            63. Amirhekmat A, Brown WE, Salinas EY, Hu JC, Athanasiou KA, Wang D. Mechanical Evaluation of Commercially Available Fibrin Sealants for Cartilage Repair. Cartilage 2024 Jun;15(2):147-155.
              doi: 10.1177/19476035231163273pubmed: 36974340google scholar: lookup
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