Analyze Diet
Journal of animal science2004; 82(7); 1986-1996; doi: 10.2527/2004.8271986x

Effects of cereal grain supplementation on apparent digestibility of nutrients and concentrations of fermentation end-products in the feces and serum of horses consuming alfalfa cubes.

Abstract: Twenty geldings (five groups; similar age and BW) were used in a completely randomized design experiment to determine effects of grain supplementation of an alfalfa-cube diet on apparent nutrient digestibility and hindgut fermentation. The geldings were housed individually, fed their diets in two equal meals (0600 and 1800), and adapted to five dietary treatments over 6 wk. The treatments were alfalfa cubes (1% of BW; DM basis) without (control) or with one of four rolled cereal grains (i.e., barley, corn, naked oats, or oats) to provide a target level of 0.4% of BW as total nonstructural carbohydrates (TNC). Due to acute laminitis, three geldings (one in the control group and two in the barley group) were excluded. Because of this and multiple incidents of gas colic, TNC level was decreased to 0.2% of BW to ensure the geldings' health throughout the adaptation (7 d) and sample collection (5 d) periods. Grain intakes varied (P < 0.05) and reflected the different TNC concentrations. Apparent digestibilities of DM, OM, CP, NDF, ADF, and cellulose were not affected (P > 0.05) by grain supplementation and averaged 63.2, 63.1, 79.5, 42.7, 39.9, and 50.3%, respectively. Regardless of the source, grain supplementation increased (P < 0.05) apparent digestibility of TNC (from 85.6 to 94.6%) and decreased (P < 0.05) fecal pH (from 7.04 to 6.74). Fecal concentrations of total VFA (mg/g of DM) were greatest for the barley and naked oats diets (averaging 11.73), intermediate for the oats diet (8.00), and least for the control and corn diets (averaging 5.00; P < 0.05). Fecal concentrations of lactate (microg/g of DM) were greatest for the barley diet (254), intermediate for the oats diet (138), and least for the remaining diets (averaging 100; P < 0.05). Fecal concentrations of NH3 N (mg/g of DM) were greatest for the naked oats diet (1.68), intermediate for the barley and oats diets (averaging 0.86), and least for the remaining diets (averaging 0.63; P < 0.05). Serum concentration of lactate was 46% higher (P < 0.05) for the control than for the grain diets (averaging 0.05 mg/100 mL). Feeding barley, corn, naked oats, and oats contributed to 13, 15, 8, and 20% higher (P < 0.05) serum NH3 N concentrations than the control diet (0.25 mg/100 mL). Higher (P < 0.05) serum concentrations of urea N (mg/100 mL) were detected for the control, barley, and naked oats diets (averaging 25.28) than for the corn or oats diets (averaging 22.21). Results suggest that horses consuming alfalfa cubes could be supplemented with rolled barley, corn, naked oats, or oats at levels not exceeding 0.2% of BW as TNC without affecting nutrient digestion or overall health negatively.
Publication Date: 2004-08-18 PubMed ID: 15309945DOI: 10.2527/2004.8271986xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the impact of grain supplementation on the digestion and metabolic processes of horses consuming alfalfa cubes. The grains under study included barley, corn, naked oats, and oats. The key conclusion is that these grains can be safely incorporated into the horses’ diet without negatively affecting the digestion or overall health of the animals.

Research Design and Methodology

  • The experimental design was a completely randomized design involving twenty geldings, divided into five groups. One group, the control, consumed alfalfa cubes without any grain supplementation, while the other four were each supplemented with a different type of rolled cereal grain to provide a targeted intake of total nonstructural carbohydrates (TNC).
  • The horses were fed two equal meals per day (at 0600 and 1800) and adapted to the diet over a six-week period.
  • However, due to cases of acute laminitis with three of the horses and several incidents of gas colic, the TNC level had to be reduced to 0.2% of the horse’s body weight to maintain their health during the adaptation and sample collection periods. This highlights the necessity of ensuring the health and safety of experimental subjects during the research process.

Results and Findings

  • The results indicated that grain intake varied depending on the different TNC concentrations in each type of grain.
  • The apparent digestibility of key nutrients, including DM, OM, CP, NDF, ADF, and cellulose, was not significantly affected by grain supplementation.
  • Regardless of the type of grain used for supplementation, the addition of grain increased the apparent digestibility of TNC and resulted in a decrease in fecal pH.
  • The study also found differences in the fecal concentrations of total Volatile Fatty Acids (VFA), lactate, and NH3 N among the various diets. For example, barley and naked oats diets had the highest total VFA concentration while lactate concentration was highest in the barley diet. Similarly, naked oats diets resulted in the highest fecal concentration of NH3 N.
  • Serum concentrations of lactate were found to be higher in the control group than in the grain diets, while serum NH3 N concentrations were higher in the diets with the various grains than in the control diet. Urea N concentrations were higher in the serum of horses fed the control, barley, and naked oats diets than in those fed the corn or oats diets.

Conclusion

  • The study concludes that supplementation of horse diets consisting of alfalfa cubes with any of the four grains (barley, corn, naked oats, or oats) does not have any significant negative effects on nutrient digestion or overall health, as long as it does not exceed 0.2% of the horse’s body weight as TNC.
  • This finding provides a valuable contribution to the understanding of equine nutrition, particularly in the context of optimizing diet composition for horses.

Cite This Article

APA
Hussein HS, Vogedes LA, Fernandez GC, Frankeny RL. (2004). Effects of cereal grain supplementation on apparent digestibility of nutrients and concentrations of fermentation end-products in the feces and serum of horses consuming alfalfa cubes. J Anim Sci, 82(7), 1986-1996. https://doi.org/10.2527/2004.8271986x

Publication

ISSN: 0021-8812
NlmUniqueID: 8003002
Country: United States
Language: English
Volume: 82
Issue: 7
Pages: 1986-1996

Researcher Affiliations

Hussein, H S
  • Department of Animal Biotechnology, University of Nevada-Reno, Reno, NV 89557, USA. hhussein@agnt1.ag.unr.edu
Vogedes, L A
    Fernandez, G C J
      Frankeny, R L

        MeSH Terms

        • Animal Feed
        • Animal Nutritional Physiological Phenomena
        • Animals
        • Avena
        • Dietary Supplements
        • Digestion
        • Edible Grain / metabolism
        • Fatty Acids, Volatile / metabolism
        • Feces / chemistry
        • Fermentation
        • Hordeum
        • Horses / blood
        • Horses / metabolism
        • Hydrogen-Ion Concentration
        • Male
        • Medicago sativa
        • Nitrogen / metabolism
        • Nutritive Value
        • Random Allocation
        • Zea mays

        Citations

        This article has been cited 7 times.
        1. Huang X, Li Q, Li X, Li C, Li J, He L, Jing H, Yang F, Li X. Effects of different grain types on nutrient apparent digestibility, glycemic responses, and fecal VFA content in weaned foals. BMC Vet Res 2025 Apr 14;21(1):273.
          doi: 10.1186/s12917-025-04716-wpubmed: 40229645google scholar: lookup
        2. Brandi LA, Nunes AT, Faleiros CA, Poleti MD, Oliveira ECM, Schmidt NT, Sousa RLM, Fukumasu H, Balieiro JCC, Brandi RA. Dietary Energy Sources Affect Cecal and Fecal Microbiota of Healthy Horses. Animals (Basel) 2024 Dec 3;14(23).
          doi: 10.3390/ani14233494pubmed: 39682460google scholar: lookup
        3. Park T, Cheong H, Yoon J, Kim A, Yun Y, Unno T. Comparison of the Fecal Microbiota of Horses with Intestinal Disease and Their Healthy Counterparts. Vet Sci 2021 Jun 17;8(6).
          doi: 10.3390/vetsci8060113pubmed: 34204317google scholar: lookup
        4. Cersosimo LM, Sullivan KE, Valdes EV. Species and individual rhinoceros affect the bacterial communities, metabolites, and nutrient composition in faeces from Southern black rhinoceros (Diceros bicornis minor) and Southern white rhinoceros (Ceratotherium simum simum) under managed care. J Anim Physiol Anim Nutr (Berl) 2022 Jan;106(1):181-193.
          doi: 10.1111/jpn.13520pubmed: 33655648google scholar: lookup
        5. Sorensen RJ, Drouillard JS, Douthit TL, Ran Q, Marthaler DG, Kang Q, Vahl CI, Lattimer JM. Effect of hay type on cecal and fecal microbiome and fermentation parameters in horses. J Anim Sci 2021 Jan 1;99(1).
          doi: 10.1093/jas/skaa407pubmed: 33515482google scholar: lookup
        6. Liu LL, Zhou XL, Yang HJ, Chen R. Effect of Dietary Forage/Concentrate Ratio on Nutrient Digestion and Energy and Protein Metabolism in Adult Donkeys. Animals (Basel) 2020 Jun 12;10(6).
          doi: 10.3390/ani10061025pubmed: 32545612google scholar: lookup
        7. Shepherd ML, Ponder MA, Burk AO, Milton SC, Swecker WS Jr. Fibre digestibility, abundance of faecal bacteria and plasma acetate concentrations in overweight adult mares. J Nutr Sci 2014;3:e10.
          doi: 10.1017/jns.2014.8pubmed: 25191602google scholar: lookup