Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes.
Abstract: Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of cyclic equibiaxial mechanical stretch on the expression of α-BK and TRPV4 channels. Results: Freshly isolated equine articular chondrocytes were subjected to mechanical stress (8% elongation at frequency of 0.5 Hz for 8 h). Western blotting was used to investigate the expression of BKCa and TRPV4 channel proteins. Mechanical stretch increased the expression of BKCa channels by 1.8 fold but TRPV4 expression was not affected. Conclusions: Upregulation of BKCa channel may be the result of direct membrane stretch or elevated intracellular Ca(2+).
Publication Date: 2014-01-29 PubMed ID: 24516787PubMed Central: PMC3913796DOI: 10.1186/2193-1801-3-59Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Case Reports
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates how exposing equine chondrocytes, the cells in horse joint cartilage, to cyclic equibiaxial mechanical stretch impacts the expression of α-BK and TRPV4 channels, which are involved in the cells’ response to mechanical stress. The findings indicate that such stretching increases the expression of BKCa channels but does not affect TRPV4 expression.
Understanding the Research Context
- Chondrocytes are cells found in the cartilage, the flexible connective tissue in joints. These cells are regularly exposed to varying degrees of pressure and have the ability to sense and respond to this mechanical stress. Yet, how they do this – a process called mechanotransduction – is not fully understood.
- The study focused on α-BK and TRPV4, two channel proteins that are believed to be involved in chondrocyte mechanotransduction. The research aimed to explore how these channels are affected by a specific type of mechanical stress: cyclic equibiaxial stretch.
Research Methodology
- The researchers used fresh equine chondrocytes for the experiment. They subjected these cells to mechanical stress by stretching them at 8% elongation at a frequency of 0.5 Hz for 8 hours.
- Following this period of stress, they used a technique called western blotting to examine the levels of BKCa and TRPV4 channel proteins in these cells. Western blotting is a common lab technique that identifies specific proteins within a sample.
Research Findings
- The results showed that expression of BKCa channels increased by 1.8 fold after being subjected to mechanical stretch. This suggests that the stretch stimulated the production of these channels.
- In contrast, the expression of TRPV4 channels was unchanged. This indicates that mechanical stretch does not influence the production of these channels in equine chondrocytes.
Conclusions and Implications
- The upregulation of BKCa channels as a result of mechanical stretch could be due to the direct effects of the stretch on the cell membrane or due to an increase in intracellular Ca(2+).
- Given the crucial role of chondrocytes in maintaining joint health, understanding the mechanotransduction mechanisms could provide insights into the pathogenesis of osteoarthritis and other joint diseases, paving the way for potential therapeutic strategies.
Cite This Article
APA
Hdud IM, Mobasheri A, Loughna PT.
(2014).
Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes.
Springerplus, 3, 59.
https://doi.org/10.1186/2193-1801-3-59 Publication
Researcher Affiliations
- School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD UK.
- School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD UK ; Medical Research Council-Arthritis Research UK Centre for Musculoskeletal Ageing Research, Leicestershire, UK.
- School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD UK ; Medical Research Council-Arthritis Research UK Centre for Musculoskeletal Ageing Research, Leicestershire, UK.
References
This article includes 17 references
- Archer CW, Francis-West P. The chondrocyte.. Int J Biochem Cell Biol 2003 Apr;35(4):401-4.
- Atherton PJ, Szewczyk NJ, Selby A, Rankin D, Hillier K, Smith K, Rennie MJ, Loughna PT. Cyclic stretch reduces myofibrillar protein synthesis despite increases in FAK and anabolic signalling in L6 cells.. J Physiol 2009 Jul 15;587(Pt 14):3719-27.
- Grandolfo M, Calabrese A, D'Andrea P. Mechanism of mechanically induced intercellular calcium waves in rabbit articular chondrocytes and in HIG-82 synovial cells.. J Bone Miner Res 1998 Mar;13(3):443-53.
- Guilak F. Biomechanical factors in osteoarthritis.. Best Pract Res Clin Rheumatol 2011 Dec;25(6):815-23.
- Huang H, Pan Y, Ye Y, Gao M, Yin Z, Luo L. Dipyrithione attenuates oleic acid-induced acute lung injury.. Pulm Pharmacol Ther 2011 Feb;24(1):74-80.
- Inoue H, Hiasa K, Samma Y, Nakamura O, Sakuda M, Iwamoto M, Suzuki F, Kato Y. Stimulation of proteoglycan and DNA syntheses in chondrocytes by centrifugation.. J Dent Res 1990 Sep;69(9):1560-3.
- Liappis AP, Gibbs KW, Nylen ES, Yoon B, Snider RH, Gao B, Becker KL. Exogenous procalcitonin evokes a pro-inflammatory cytokine response.. Inflamm Res 2011 Feb;60(2):203-7.
- Liedtke W, Choe Y, Martí-Renom MA, Bell AM, Denis CS, Sali A, Hudspeth AJ, Friedman JM, Heller S. Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor.. Cell 2000 Oct 27;103(3):525-35.
- Martina M, Mozrzymas JW, Vittur F. Membrane stretch activates a potassium channel in pig articular chondrocytes.. Biochim Biophys Acta 1997 Oct 23;1329(2):205-10.
- Mobasheri A, Lewis R, Maxwell JE, Hill C, Womack M, Barrett-Jolley R. Characterization of a stretch-activated potassium channel in chondrocytes.. J Cell Physiol 2010 May;223(2):511-8.
- Mouw JK, Imler SM, Levenston ME. Ion-channel regulation of chondrocyte matrix synthesis in 3D culture under static and dynamic compression.. Biomech Model Mechanobiol 2007 Jan;6(1-2):33-41.
- Nilius B, Owsianik G. The transient receptor potential family of ion channels.. Genome Biol 2011;12(3):218.
- Phan MN, Leddy HA, Votta BJ, Kumar S, Levy DS, Lipshutz DB, Lee SH, Liedtke W, Guilak F. Functional characterization of TRPV4 as an osmotically sensitive ion channel in porcine articular chondrocytes.. Arthritis Rheum 2009 Oct;60(10):3028-37.
- Urban JP. The chondrocyte: a cell under pressure.. Br J Rheumatol 1994 Oct;33(10):901-8.
- Veys K, Labro AJ, De Schutter E, Snyders DJ. Quantitative single-cell ion-channel gene expression profiling through an improved qRT-PCR technique combined with whole cell patch clamp.. J Neurosci Methods 2012 Jul 30;209(1):227-34.
- Wang IC, Ueng SW, Lin SS, Niu CC, Yuan LJ, Su CI, Chen CH, Chen WJ. Effect of hyperbaric oxygenation on intervertebral disc degeneration: an in vitro study with human lumbar nucleus pulposus.. Spine (Phila Pa 1976) 2011 Nov 1;36(23):1925-31.
- Xiang D, Guo Y, Zhang J, Gao J, Lu H, Zhu S, Wu M, Yu Y, Han W. Interleukin-1 receptor antagonist attenuates cyclophosphamide-induced mucositis in a murine model.. Cancer Chemother Pharmacol 2011 Jun;67(6):1445-53.
Citations
This article has been cited 3 times.- Takács R, Kovács P, Ebeid RA, Almássy J, Fodor J, Ducza L, Barrett-Jolley R, Lewis R, Matta C. Ca(2+)-Activated K(+) Channels in Progenitor Cells of Musculoskeletal Tissues: A Narrative Review.. Int J Mol Sci 2023 Apr 5;24(7).
- Hdud IM, Loughna PT. Influence of 1α, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes.. J Anim Sci Technol 2014;56:33.
- McNulty AL, Leddy HA, Liedtke W, Guilak F. TRPV4 as a therapeutic target for joint diseases.. Naunyn Schmiedebergs Arch Pharmacol 2015 Apr;388(4):437-50.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
