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Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes.
Abstract: Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of cyclic equibiaxial mechanical stretch on the expression of α-BK and TRPV4 channels. Results: Freshly isolated equine articular chondrocytes were subjected to mechanical stress (8% elongation at frequency of 0.5 Hz for 8 h). Western blotting was used to investigate the expression of BKCa and TRPV4 channel proteins. Mechanical stretch increased the expression of BKCa channels by 1.8 fold but TRPV4 expression was not affected. Conclusions: Upregulation of BKCa channel may be the result of direct membrane stretch or elevated intracellular Ca(2+).
Publication Date: 2014-01-29 PubMed ID: 24516787PubMed Central: PMC3913796DOI: 10.1186/2193-1801-3-59Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
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Summary
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The research article investigates how exposing equine chondrocytes, the cells in horse joint cartilage, to cyclic equibiaxial mechanical stretch impacts the expression of α-BK and TRPV4 channels, which are involved in the cells’ response to mechanical stress. The findings indicate that such stretching increases the expression of BKCa channels but does not affect TRPV4 expression.
Understanding the Research Context
- Chondrocytes are cells found in the cartilage, the flexible connective tissue in joints. These cells are regularly exposed to varying degrees of pressure and have the ability to sense and respond to this mechanical stress. Yet, how they do this – a process called mechanotransduction – is not fully understood.
- The study focused on α-BK and TRPV4, two channel proteins that are believed to be involved in chondrocyte mechanotransduction. The research aimed to explore how these channels are affected by a specific type of mechanical stress: cyclic equibiaxial stretch.
Research Methodology
- The researchers used fresh equine chondrocytes for the experiment. They subjected these cells to mechanical stress by stretching them at 8% elongation at a frequency of 0.5 Hz for 8 hours.
- Following this period of stress, they used a technique called western blotting to examine the levels of BKCa and TRPV4 channel proteins in these cells. Western blotting is a common lab technique that identifies specific proteins within a sample.
Research Findings
- The results showed that expression of BKCa channels increased by 1.8 fold after being subjected to mechanical stretch. This suggests that the stretch stimulated the production of these channels.
- In contrast, the expression of TRPV4 channels was unchanged. This indicates that mechanical stretch does not influence the production of these channels in equine chondrocytes.
Conclusions and Implications
- The upregulation of BKCa channels as a result of mechanical stretch could be due to the direct effects of the stretch on the cell membrane or due to an increase in intracellular Ca(2+).
- Given the crucial role of chondrocytes in maintaining joint health, understanding the mechanotransduction mechanisms could provide insights into the pathogenesis of osteoarthritis and other joint diseases, paving the way for potential therapeutic strategies.
Cite This Article
APA
Hdud IM, Mobasheri A, Loughna PT.
(2014).
Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes.
Springerplus, 3, 59.
https://doi.org/10.1186/2193-1801-3-59 Publication
Researcher Affiliations
- School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD UK.
- School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD UK ; Medical Research Council-Arthritis Research UK Centre for Musculoskeletal Ageing Research, Leicestershire, UK.
- School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD UK ; Medical Research Council-Arthritis Research UK Centre for Musculoskeletal Ageing Research, Leicestershire, UK.
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Citations
This article has been cited 3 times.- Takács R, Kovács P, Ebeid RA, Almássy J, Fodor J, Ducza L, Barrett-Jolley R, Lewis R, Matta C. Ca(2+)-Activated K(+) Channels in Progenitor Cells of Musculoskeletal Tissues: A Narrative Review. Int J Mol Sci 2023 Apr 5;24(7).
- Hdud IM, Loughna PT. Influence of 1α, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes. J Anim Sci Technol 2014;56:33.
- McNulty AL, Leddy HA, Liedtke W, Guilak F. TRPV4 as a therapeutic target for joint diseases. Naunyn Schmiedebergs Arch Pharmacol 2015 Apr;388(4):437-50.
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