Effects of exercise and oral antioxidant supplementation enriched in (n-3) fatty acids on blood oxidant markers and erythrocyte membrane fluidity in horses.
Abstract: The aim of this study was to investigate in a placebo-controlled field study the effect of a (n-3)-vitamin supplementation on erythrocyte membrane fluidity (EMF), oxidant/antioxidant markers and plasmatic omega3/omega6 fatty acid ratio (FAR) in 12 eventing horses. Venous blood was sampled at rest before (PRE) and after (POST) a three week treatment period with either the supplement (group S, n=6) or a placebo (group P, n=6) as well as after 15min (POST E15') and 24h (POST E24h) after a standardised exercise test. The following markers were analysed: EMF, plasma antioxidant capacity of water and lipid soluble components, ascorbic acid, uric acid (UA), glutathione (reduced: GSH, oxidised: GSSG), vitamin E (Vit E), beta-carotene, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity, selenium, copper (Cu), zinc (Zn), oxidised proteins (Protox), lipid peroxides (Pool) and FAR. EMF did not differ between group S and P after treatment, but GPx remained unchanged in group S whereas it decreased in group P and plasma Cu/Zn ratio remained unchanged whereas it increased in group P. FAR were significantly increased in group S. Exercise induced a significant decrease of EMF (POST vs. E24h) in both groups, but which was significantly lower at E15' in group S than in group P. Exercise induced a significant increase of UA and ACW (POST vs. E15') and Protox (POST vs. E24h) in both groups. An exercise-related decrease in GSH and Pool (POST vs. E15') was found in group P, whereas Vit E and FAR (POST vs. E24h) significantly decreased in both groups. The study showed that exercise induced a decrease in EMF in horses associated with changes of blood oxidative balance. The (omega-3)-vitamin supplementation tested improved the oxidative balance poorly but delayed the exercise-induced decrease of EMF and increased the FAR.
Publication Date: 2006-11-15 PubMed ID: 17110141DOI: 10.1016/j.tvjl.2006.06.001Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research study investigated the effects of an (n-3)-vitamin supplement on the levels of blood oxidant markers and erythrocyte membrane fluidity (EMF) in horses. In the process, the researchers discovered that while the supplement didn’t drastically improve the oxidative balance in the horses, it did delay an exercise-induced decrease in EMF and increased the omega3/omega6 fatty acid ratio (FAR).
Research Context and Methodology
- This study involved a placebo-controlled field test to study the impact of a supplement rich in (n-3) fatty acids on blood oxidant markers, EMF, and the plasmatic omega3/omega6 FAR of 12 eventing horses.
- The horses were divided into two groups: a supplement group (S) and a placebo group (P), with six horses in each group.
- Before and after a three-week treatment period, venous blood samples were taken from each horse. In addition, samples were taken 15 minutes (POST E15′) and 24 hours (POST E24h) after a standardized exercise test.
- The researchers studied several oxidant/antioxidant markers, including EMF, plasma antioxidant capacity of water and lipid-soluble components, ascorbic acid, uric acid (UA), glutathione (both reduced: GSH and oxidised: GSSG), vitamin E, beta-carotene, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity, selenium, copper (Cu), zinc (Zn), oxidised proteins (Protox), lipid peroxides (Pool), and FAR.
Findings
- After the treatment, no significant difference was observed between groups S and P in terms of EMF. However, GPx remained unchanged in group S, while it decreased in group P. Likewise, the plasma Cu/Zn ratio remained the same in group S, but increased in group P.
- FAR was significantly higher in group S.
- Both groups noted a significant decrease in EMF (POST vs E24h) after exercising. However, the decrease was significantly less at E15′ in group S than in group P.
- Both groups showed an increase in UA and ACW (POST vs E15′) and Protox (POST vs E24h) after exercise. A decrease in GSH and Pool (POST vs E15′) was recorded in group P, while Vit E and FAR (POST vs E24h) significantly decreased in both groups after exercising.
Conclusion
- The study confirmed that exercising leads to a drop in EMF in horses, which is linked to changes in blood oxidative balance. Although the (n-3)-vitamin supplementation used in the study had a minimal impact on improving the oxidative balance, it played a role in delaying the exercise-induced decrease of EMF and increasing the FAR.
Cite This Article
APA
De Moffarts B, Portier K, Kirschvink N, Coudert J, Fellmann N, van Erck E, Letellier C, Motta C, Pincemail J, Art T, Lekeux P.
(2006).
Effects of exercise and oral antioxidant supplementation enriched in (n-3) fatty acids on blood oxidant markers and erythrocyte membrane fluidity in horses.
Vet J, 174(1), 113-121.
https://doi.org/10.1016/j.tvjl.2006.06.001 Publication
Researcher Affiliations
- Department for Functional Sciences B41, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
MeSH Terms
- Animals
- Antioxidants / administration & dosage
- Dietary Supplements
- Erythrocyte Membrane / drug effects
- Erythrocyte Membrane / physiology
- Fatty Acids, Omega-3 / administration & dosage
- Fatty Acids, Omega-6 / administration & dosage
- Female
- Horses / blood
- Male
- Physical Conditioning, Animal
Citations
This article has been cited 5 times.- De Palo P, Maggiolino A, Albenzio M, Casalino E, Neglia G, Centoducati G, Tateo A. Survey of biochemical and oxidative profile in donkey foals suckled with one natural and one semi-artificial technique. PLoS One 2018;13(6):e0198774.
- Berzosa C, Gómez-Trullén EM, Piedrafita E, Cebrián I, Martínez-Ballarín E, Miana-Mena FJ, Fuentes-Broto L, García JJ. Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans. Eur J Appl Physiol 2011 Jun;111(6):1127-33.
- Alberghina D, Piccione G, Amorini AM, D'Urso S, Longo S, Picardi M, Tavazzi B, Lazzarino G. Modulation of circulating purines and pyrimidines by physical exercise in the horse. Eur J Appl Physiol 2011 Mar;111(3):549-56.
- Bruschetta G, Alberghina D, Nastasi G, Rotondo E, Ferlazzo AM. Characterization of phospholipid composition of pig plasma and erythrocyte membranes. Vet Res Commun 2008 Sep;32 Suppl 1:S115-8.
- Berzosa C, Bascuas PJ, Piedrafita E. Effects of Melatonin Administration on Physical Performance and Biochemical Responses Following Exhaustive Treadmill Exercise. Curr Issues Mol Biol 2024 Nov 30;46(12):13647-13661.
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