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Home / Equine Research Bank / Can Vet J / Effects of exercise, furosemide, blood depletion, and reinfu...
The Canadian veterinary journal = La revue veterinaire canadienne2020; 61(11); 1181-1185;

Effects of exercise, furosemide, blood depletion, and reinfusion on body fluid compartment volumes in horses.

Abstract: High pulmonary blood pressure contributes to exercise-induced pulmonary hemorrhage. The objective of this study was to use bioimpedance spectroscopy to assess body fluid compartment volumes under 3 conditions in 6 racehorses: i) Pre- and post-supramaximal treadmill exercise (control); ii) Exercise 4 hours after furosemide (0.5 mg/kg body weight, IV); iii) Exercise, removal of ~14 L of blood and subsequent reinfusion of the blood. Statistical analysis used linear mixed effects models. Body compartment volumes did not change during the control runs. Total body water (TBW) ( = 0.007, = 0.007), extracellular fluid (ECF) ( = 0.003, = 0.003), and intracellular fluid (ICF) volumes ( = 0.04, = 0.04) decreased pre- and post-exercise following furosemide administration. The ICF trended to decrease ( = 0.07) after slow removal of blood. Blood reinfusion increased TBW ( = 0.02, = 0.02) and ICF ( = 0.005, = 0.005) pre- and post-exercise. . Une pression sanguine pulmonaire élevée contribue à des hémorragies pulmonaires induites par l’exercice. L’objectif de la présente étude était d’utiliser la spectroscopie à bio-impédance pour évaluer les volumes des liquides corporels compartimentés sous trois conditions chez six chevaux de course : i) Pré- et post-supramaximal exercice au tapis roulant (témoin); ii) Exercice 4 h après administration de furosémide (0,5 mg/kg de poids corporel, IV); iii) Exercice, retrait d’environ 14 L de sang et ré-infusion subséquente du sang. Les analyses statistiques utilisaient des modèles linéaires à effets mixtes. Les volumes des compartiments corporels n’ont pas changé durant les essais témoins. Les volumes de la quantité totale d’eau corporelle (TBW) ( = 0,007, = 0,007), de liquide extracellulaire (ECF) ( = 0,003, = 0,003) et liquide intracellulaire (ICF) ( = 0,04, = 0,04) ont diminué pré- et post-exercice à la suite de l’administration de furosémide. L‘ICF avait tendance à diminuer ( = 0,07) à la suite du lent retrait de sang. La ré-infusion de sang augmenta la TBW ( = 0,02, = 0,02) et l’ICF ( = 0,005, = 0,005) pré- et post-exercice.(Traduit par D Serge Messier).
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Publication Date: 2020-11-06 PubMed ID: 33149356PubMed Central: PMC7560766
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Summary

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The research investigated the impact of exercise, the use of the drug furosemide, and the removal and reinfusion of blood on the volumes of body fluid compartments in racehorses, using a technique called bioimpedance spectroscopy.

Introduction and Methodology

  • This study was carried out to understand the shifts in body fluid compartment volumes in racehorses under three different conditions: pre- and post-intense (supramaximal) treadmill exercise (control), exercise performed four hours post-administration of furosemide – a diuretic and antihypertensive drug, and lastly, physical exertion followed by removal and subsequent reinfusion of approximately 14 liters of blood.
  • The research was conducted on six racehorses.
  • Body fluid compartment volumes were evaluated using bioimpedance spectrometry, a non-invasive technique that measures resistance to an applying electrical current to determine the amount of water in the body.

Results and Discussion

  • No change was observed in body compartment volumes during the control runs, where the horses performed supramaximal exercise.
  • A decrease in total body water (TBW), extracellular fluid (ECF), and intracellular fluid (ICF) volumes was noted both pre- and post-exercise after administrating furosemide. It is a known fact that furosemide causes loss of body water, thus the results were as anticipated.
  • The third condition, i.e., slow removal of blood after exercise, tended to decrease the volume of ICF.
  • The reinfusion of blood after removal led to an increase in total body water (TBW) and intracellular fluid (ICF) volumes both before and after exercise.

Conclusions

  • The outcomes of this study contribute to the understanding of fluid dynamics in racehorses under various conditions. These insights can help equine practitioners design better conditioning and recovery practices for these high-performance animals.
  • Moreover, understanding the impact of diuretics like furosemide on racehorses can lead to a more informed usage of such drugs for therapeutic purposes.

Cite This Article

APA
McCrae P, Guigand C, Jones KB, Thueson ES, Troudt TA, Warlick LM, Sides RH, Jones JH, Bayly WM, Léguillette R. (2020). Effects of exercise, furosemide, blood depletion, and reinfusion on body fluid compartment volumes in horses. Can Vet J, 61(11), 1181-1185.

Publication

The Canadian veterinary journal = La revue veterinaire canadienne
ISSN: 0008-5286
NlmUniqueID: 0004653
Country: Canada
Language: English
Volume: 61
Issue: 11
Pages: 1181-1185

Researcher Affiliations

McCrae, Persephone
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Guigand, Charline
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Jones, K Blythe
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Thueson, Emily S
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Troudt, Tristan A
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Warlick, Linnea M
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Sides, Raymond H
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Jones, James H
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Bayly, Warwick M
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).
Léguillette, Renaud
  • University of Calgary, VCDS, 2500 University Drive NW, Calgary, Alberta T2N 1N4 (McCrae, Guigand, Léguillette); Washington State University, VCS, PO Box 6610, Pullman, Washington 99164-6610, USA (K.B. Jones, Thueson, Troudt, Warlick, Sides, Bayly); University of California, Surgical & Radiological Sciences, 944 Garrod Drive, Davis, California 95616, USA (J.H. Jones).

MeSH Terms

  • Animals
  • Body Fluid Compartments
  • Furosemide / therapeutic use
  • Hemorrhage / veterinary
  • Horse Diseases / therapy
  • Horses

References

This article includes 20 references
  1. Hinchcliff KW, Couetil LL, Knight PK, Morley PS, Robinson NE, Sweeney CR, van Erck E. Exercise induced pulmonary hemorrhage in horses: American College of Veterinary Internal Medicine consensus statement.. J Vet Intern Med 2015 May-Jun;29(3):743-58.
    pmc: PMC4895427pubmed: 25996660doi: 10.1111/jvim.12593google scholar: lookup
  2. Hinchcliff K. Exercise-induced pulmonary hemorrhage. Proc Annu Conv Am Assoc Equine Pract 2005; pp. 342–347.
  3. Persson S. On blood volume and working capacity in horses. Studies of methodology and physiological and pathological variations.. Acta Vet Scand 1967;:Suppl 19:9-189.
    pubmed: 4863601
  4. Rivas LJ, Hinchcliff KW. Effect of furosemide and subsequent intravenous fluid administration on right atrial pressure of splenectomized horses.. Am J Vet Res 1997 Jun;58(6):632-5.
    pubmed: 9185971
  5. Hinchcliff KW, Morley PS, Guthrie AJ. Efficacy of furosemide for prevention of exercise-induced pulmonary hemorrhage in Thoroughbred racehorses.. J Am Vet Med Assoc 2009 Jul 1;235(1):76-82.
    pubmed: 19566461doi: 10.2460/javma.235.1.76google scholar: lookup
  6. Ward LC, White KJ, van der Aa Kuhle K, Cawdell-Smith J, Bryden WL. Body composition assessment in horses using bioimpedance spectroscopy.. J Anim Sci 2016 Feb;94(2):533-41.
    pubmed: 27065123doi: 10.2527/jas.2015-9837google scholar: lookup
  7. Forro M, Cieslar S, Ecker GL, Walzak A, Hahn J, Lindinger MI. Total body water and ECFV measured using bioelectrical impedance analysis and indicator dilution in horses.. J Appl Physiol (1985) 2000 Aug;89(2):663-71.
    pubmed: 10926652doi: 10.1152/jappl.2000.89.2.663google scholar: lookup
  8. McKeen G, Lindinger MI. Prediction of hydration status using multi-frequency bioelectrical impedance analysis during exercise and recovery in horses. Equine Comp Exerc Physiol 2004;1:199–209.
  9. Lundvall J, Mellander S, Westling H, White T. Fluid transfer between blood and tissues during exercise.. Acta Physiol Scand 1972 Jun;85(2):258-69.
    pubmed: 5049421doi: 10.1111/j.1748-1716.1972.tb05259.xgoogle scholar: lookup
  10. Watson PD, Garner RP, Ward DS. Water uptake in stimulated cat skeletal muscle.. Am J Physiol 1993 Apr;264(4 Pt 2):R790-6.
    pubmed: 8476122doi: 10.1152/ajpregu.1993.264.4.r790google scholar: lookup
  11. Kowalchuk JM, Heigenhauser GJ, Lindinger MI, Sutton JR, Jones NL. Factors influencing hydrogen ion concentration in muscle after intense exercise.. J Appl Physiol (1985) 1988 Nov;65(5):2080-9.
    pubmed: 3145275doi: 10.1152/jappl.1988.65.5.2080google scholar: lookup
  12. Waller A, Lindinger MI. Hydration of exercised standardbred racehorses assessed noninvasively using multi-frequency bioelectrical impedance analysis.. Equine Vet J Suppl 2006 Aug;(36):285-90.
    pubmed: 17402433doi: 10.1111/j.2042-3306.2006.tb05554.xgoogle scholar: lookup
  13. Waller A, Lindinger MI. Time course and magnitude of fluid and electrolyte shifts during recovery from high-intensity exercise in Standardbred racehorses. Equine Comp Exerc Physiol 2005;2:77–87.
  14. Lindinger MI, McKeen G, Ecker GL. Time course and magnitude of changes in total body water, extracellular fluid volume, intracellular fluid volume and plasma volume during submaximal exercise and recovery in horses. Comp Exerc Physiol 2004;1:131–139.
  15. Forro M, Lindinger MI. Frusemide results in an extracellular to intracellular fluid shift in horses.. Equine Vet J Suppl 2006 Aug;(36):245-53.
    pubmed: 17402426doi: 10.1111/j.2042-3306.2006.tb05547.xgoogle scholar: lookup
  16. Hinchcliff KW, McKeever KH, Muir WW 3rd. Furosemide-induced changes in plasma and blood volume of horses.. J Vet Pharmacol Ther 1991 Dec;14(4):411-7.
    pubmed: 1774817doi: 10.1111/j.1365-2885.1991.tb00855.xgoogle scholar: lookup
  17. Tobin T, Roberts BL, Sweczek TW, Crisman M. The pharmacology of furosemide in the horse. III. Dose and time response relationships, effects of repeated dosing, and performance effects. J Equine Med Surg 1978;2:216–226.
  18. Fielding CL, Magdesian KG, Carlson GP, Ruby RE, Rhodes DM. Estimation of acute fluid shifts using bioelectrical impedance analysis in horses.. J Vet Intern Med 2007 Jan-Feb;21(1):176-83.
    pubmed: 17338166doi: 10.1892/0891-6640(2007)21[176:eoafsu]2.0.co;2google scholar: lookup
  19. Sneddon JC, VanderWalt J, Mitchell G. Effect of dehydration on the volumes of body fluid compartments in horses. J Arid Environ 1993;24:397–408.
  20. McGuill MW, Rowan AN. Biological effects of blood loss: Implications for sampling volumes and techniques. Perspect Anim Use 1987;31:5–20.

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