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Research in veterinary science2018; 118; 295-303; doi: 10.1016/j.rvsc.2018.02.008

Effects of grain species, genotype and starch quantity on the postprandial plasma amino acid response in horses.

Abstract: Postprandial alterations of plasma amino acid (PAA) levels partly reflect a temporal contribution of the feed. How cereal grains affect PAA levels is not known. We hypothesized that a meal of cereal grains causes a temporal increase of PAA, affected by grain species, grain genotype and meal size. Six mares were used in three consecutive trials, receiving four oats, barley and maize genotypes, respectively. Individual grain genotypes were provided as 3 meal sizes corresponding to 1.0, 1.5 or 2.0 g starch/kg body weight. Meadow hay (1.5 kg/100 kg body weight) was offered daily. At the test days, 1 kg hay was fed 60 min prior to the grain meal. Blood samples were taken before grain feeding (0 min) and 30, 60, 90, 120, 180, 240 and 300 min thereafter. Subsequently, the remaining hay was offered. The genotype × starch quantity (i.e., meal size) interaction had a major effect on postprandial PAA concentrations (P < 0.05). Availability of amino acids (AA), ingested from different grain genotypes, apparently differed at both the digestive and post-digestive level. Thus, AA supply from grain feeding can better be assessed on the genotype level. The concentrations of most PAA increased rapidly with a postprandial maximum at around 30 min. Hay feeding might have an underrated capability for AA provision because increases of PAA levels were initialized already by ingestion of a 1 kg hay. It remains unclear which portion of the PAA kinetics response originates from hay feeding and which one from the cereal grain meal.
Publication Date: 2018-03-21 PubMed ID: 29547728DOI: 10.1016/j.rvsc.2018.02.008Google Scholar: Lookup
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  • Journal Article

Summary

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The research article examines the impact of various grain species, their genotypes, and starch quantity on the level of plasma amino acids in horses after meals. The study contends that the availability of amino acids varies depending on the type of grain and its amount consumed.

Research Approach

  • The researchers utilized six mares in three consecutive trials. Each trial involved feeding the horses different genotypes of oats, barley, and maize respectively.
  • The grain genotypes were offered in three meal sizes that corresponded to 1.0, 1.5 or 2.0 g starch/kg body weight.
  • The horses were also offered meadow hay (1.5 kg/100 kg body weight) daily. On test days, 1 kg of hay was fed to the horses 60 minutes prior to grain feeding.
  • Blood samples from the horses were then taken before grain feeding (0 min) and then at 30, 60, 90, 120, 180, 240, and 300 minutes after the grain feeding. The remaining hay was then offered to the horses.

Key Findings

  • The research found that there was a significant interaction between the grain genotype and starch quantity (what is referred to as “meal size”) in regards to post-meal plasma amino acid concentrations.
  • Additionally, the study revealed distinctions in the availability of amino acids at both the digestive and post-digestive level, depending on the respective grain genotypes that were ingested.
  • The levels of most plasma amino acids increased quickly after the meal, reaching a maximum around the 30 minute mark.
  • The study also suggests that hay feeding might provide a higher amino acid provision than previously thought, considering the immediate increase in plasma amino acids if the intake is already at the 1 kg hay.
  • However, it remains unclear as to what portion of the plasma amino acid kinetics response originates from the hay feeding and what portion comes from the cereal grain meals.

Cite This Article

APA
Bachmann M, Czetö A, Romanowski K, Vernunft A, Wensch-Dorendorf M, Wolf P, Metges CC, Zeyner A. (2018). Effects of grain species, genotype and starch quantity on the postprandial plasma amino acid response in horses. Res Vet Sci, 118, 295-303. https://doi.org/10.1016/j.rvsc.2018.02.008

Publication

ISSN: 1532-2661
NlmUniqueID: 0401300
Country: England
Language: English
Volume: 118
Pages: 295-303
PII: S0034-5288(17)31148-7

Researcher Affiliations

Bachmann, Martin
  • Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
Czetö, Aniko
  • Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
Romanowski, Kristin
  • Chair for Nutritional Physiology and Animal Nutrition, Faculty of Agricultural and Environmental Sciences, University of Rostock, 18059 Rostock, Germany.
Vernunft, Andreas
  • Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany.
Wensch-Dorendorf, Monika
  • Institute of Agricultural and Nutritional Sciences, Biometrics and Informatics in Agriculture Group, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
Wolf, Petra
  • Chair for Nutritional Physiology and Animal Nutrition, Faculty of Agricultural and Environmental Sciences, University of Rostock, 18059 Rostock, Germany.
Metges, Cornelia C
  • Institute of Nutritional Physiology 'Oskar Kellner', Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany.
Zeyner, Annette
  • Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany. Electronic address: annette.zeyner@landw.uni-halle.de.

MeSH Terms

  • Amino Acids / blood
  • Animal Feed / analysis
  • Animals
  • Diet
  • Digestion
  • Edible Grain / genetics
  • Edible Grain / metabolism
  • Female
  • Genotype
  • Horses / metabolism
  • Postprandial Period
  • Starch / metabolism

Citations

This article has been cited 2 times.
  1. Bockisch F, Taubert J, Coenen M, Vervuert I. Protein Evaluation of Feedstuffs for Horses. Animals (Basel) 2023 Aug 14;13(16).
    doi: 10.3390/ani13162624pubmed: 37627415google scholar: lookup
  2. Dänicke S, Saltzmann J, Liermann W, Glatter M, Hüther L, Kersten S, Zeyner A, Feige K, Warnken T. Evaluation of Inner Exposure of Horses to Zearalenone (ZEN), Deoxynivalenol (DON) and Their Metabolites in Relation to Colic and Health-Related Clinical-Chemical Traits. Toxins (Basel) 2021 Aug 23;13(8).
    doi: 10.3390/toxins13080588pubmed: 34437459google scholar: lookup