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Effects of low-dose G-CSF formulation on hematology in healthy horses after long-distance transportation.
Abstract: The present study evaluated the effects of single-dose filgrastim on hematology in 16 healthy horses after long-distance transportation. Horses were assigned to receive filgrastim (0.23 µg/kg, SC, once; G-CSF group; n=8) or saline (0.9% NaCl) solution (0.3 ml, SC, once; control group; n=8) ≤ 1 hr before transportation. Horses were transported 2,530 km using commercial vans over the course of approximately 44 hr. Clinical examinations and hematologic analyses were performed on all horses before and after transportation. Because the post-transportation white blood cell counts and bacillary neutrophil to segmented neutrophil ratio were significantly higher in the G-CSF group, filgrastim may have promoted the mobilization of neutrophils from marrow. Filgrastim deserves a further study for efficacy in preventing horse shipping fever.
Publication Date: 2015-01-19 PubMed ID: 25648988PubMed Central: PMC4427757DOI: 10.1292/jvms.14-0586Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Controlled Clinical Trial
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research paper studies the impact of a drug called filgrastim on healthy horses after they’ve been transported over long distances. The findings suggest that this drug has the potential to support the mobilization of neutrophils, which could ultimately help prevent horse shipping fever.
Study Methodology
- The study involved 16 healthy horses in total, divided into two groups of 8 horses each. One group was made the control group that was given a saline solution, while the other group, known as the G-CSF group, was administered with filgrastim.
- The dose of filgrastim was kept at 0.23 µg/kg and it was injected subcutaneously once. The saline solution was also injected in a similar fashion.
- Both the control and test solutions were injected into the horses no longer than 1 hour before they were transported.
- The horses were then transported over a distance of 2,530 km using commercial transportation services. The journey lasted for about 44 hours.
- To gauge the effects of filgrastim and transportation, clinical examinations and hematologic analyses were performed on all the horses before and after their journey.
Results
- The post-transportation results revealed an important finding: the white blood cell counts and bacillary neutrophil to segmented neutrophil ratio were significantly higher in the G-CSF group rather than the control group.
- This suggested that filgrastim potentially stimulated the mobilization of neutrophils from the bone marrow of the horses in the G-CSF group.
- Neutrophils are a type of white blood cell which are crucial to the immune system as they help to fight off infections.
- Mobilization of neutrophils means an increase in the production and function of these cells, thus bolstering the immune system and potentially protecting the horses against infections and diseases that can occur post-transporation, such as horse shipping fever.
Conclusion and Further Research
- Based on the results, it was concluded that filgrastim could play a significant role in strengthening the immune system of horses transported over long distances and reducing their susceptibility to ailments like shipping fever.
- However, the researchers made it clear that more extensive studies on filgrastim’s efficacy are needed before any conclusive statements can be made.
Cite This Article
APA
Endo Y, Hobo S, Korosue K, Ootsuka K, Kitauchi A, Kikkawa R, Hidaka Y, Hagio M, Tsuzuki N.
(2015).
Effects of low-dose G-CSF formulation on hematology in healthy horses after long-distance transportation.
J Vet Med Sci, 77(4), 507-509.
https://doi.org/10.1292/jvms.14-0586 Publication
Researcher Affiliations
- Miyazaki Yearling Training Farm, Japan Racing Association, 2347, Oharu, Hanagashima-cho, Miyazaki-shi, Miyazaki 880-0036, Japan.
MeSH Terms
- Animals
- Drug Administration Schedule
- Female
- Filgrastim / pharmacology
- Horses / blood
- Male
- Time Factors
- Transportation
References
This article includes 12 references
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Citations
This article has been cited 1 times.- Aponte PM, Gutierrez-Reinoso MA, Sanchez-Cepeda EG, Garcia-Herreros M. Differential role of r-met-hu G-CSF on male reproductive function and development in prepubertal domestic mammals. PLoS One 2019;14(9):e0222871.
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