Effects of phenylbutazone on bone activity and formation in horses.
Abstract: To determine the effects of phenylbutazone (PBZ) on bone activity and bone formation in horses. Methods: 12 healthy 1- to 2-year-old horses. Methods: Biopsy was performed to obtain unicortical bone specimens from 1 tibia on day 0 and from the contralateral tibia on day 14. Fluorochromic markers were administered IV 2 days prior to and on days 0, 10, 15, and 25 after biopsy was performed. Six horses received PBZ (4.4 mg/kg of body weight, PO, q 12 h) and 6 horses were used as controls. All horses were euthanatized on day 30 and tissues from biopsy sites, with adjacent cortical bone, were collected. Osteonal density and activity, mineral apposition rate (MAR), and percentage of mineralized tissue filling the biopsy-induced defects in cortical bone were assessed. Serum samples from all horses were analyzed for bone-specific alkaline phosphatase activity and concentration of PBZ. Results: MAR was significantly decreased in horses treated with PBZ. Regional acceleratory phenomenon was observed in cortical bone in both groups but was significantly decreased in horses treated with PBZ. Osteonal activity was similar at all time points in all horses. In control horses, percentage of mineralized tissue filling the cortical defects was significantly greater in defects present for 30 days, compared with defects present for 14 days. Differences in percentage of mineralized tissue were not detected in horses treated with PBZ. Conclusions: PBZ decreased MAR in cortical bone and appeared to decrease healing rate of cortical defects in horses.
Publication Date: 2000-05-10 PubMed ID: 10803649DOI: 10.2460/ajvr.2000.61.537Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates how phenylbutazone (PBZ), a drug frequently used to treat pain and inflammation in horses, affects bone activity and formation. The results indicate that PBZ may slow down bone healing rate in horses and decrease mineralization rate in their bones.
Methodology
- The researchers selected a total of 12 healthy horses between 1 to 2 years old for this study.
- A bone biopsy was performed to take specimens from one of the tibia on day 0 and the other on day 14.
- Fluorochromic markers were injected two days prior to and on four different days after the biopsy.
- The horses were then divided randomly into two groups: one group was administered PBZ and the other served as the control group.
- All the horses were euthanatized on day 30 for tissue collection from biopsy sites, alongside adjacent cortical bone.
Results
- Some key metrics were observed: osteonal density and activity, the mineral apposition rate (MAR), and the percentage of mineralized tissue filling the biopsy-induced defects.
- The results showed that MAR was significantly decreased in the horses that were treated with PBZ as compared to the ones who were not treated with PBZ.
- The phenomenon called “Regional acceleratory phenomenon” was noticed in the cortical bone in both groups. This phenomenon refers to a condition where local bone turnover rate is increased. However, this increase was less noticeable in horses treated with PBZ.
- No difference was noticed in the osteonal activity at all the time points in both groups.
- The percentage of mineralized tissue filling the biopsy-induced cortical defects was higher in the control group horses. The cortical defects in these horses were present for 30 days as compared to the ones which were present for 14 days. This difference was not noticed in the horses treated with PBZ.
Conclusion
- The study concludes that PBZ decreases the MAR in the cortical bone in horses.
- Also, it seems that PBZ might decrease the healing rate of cortical defects in horses.
Cite This Article
APA
Rohde C, Anderson DE, Bertone AL, Weisbrode SE.
(2000).
Effects of phenylbutazone on bone activity and formation in horses.
Am J Vet Res, 61(5), 537-543.
https://doi.org/10.2460/ajvr.2000.61.537 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, The Ohio State University, Columbus 43210, USA.
MeSH Terms
- Alkaline Phosphatase / blood
- Animals
- Anti-Inflammatory Agents, Non-Steroidal / pharmacology
- Anti-Inflammatory Agents, Non-Steroidal / therapeutic use
- Biopsy / veterinary
- Bone Density / drug effects
- Bone Remodeling / drug effects
- Chromatography, High Pressure Liquid / veterinary
- Female
- Fluoresceins / chemistry
- Fluorescent Dyes / chemistry
- Fracture Healing / drug effects
- Fractures, Bone / drug therapy
- Fractures, Bone / surgery
- Fractures, Bone / veterinary
- Haversian System / pathology
- Horse Diseases / drug therapy
- Horses
- Image Processing, Computer-Assisted
- Immunoassay / veterinary
- Male
- Oxytetracycline / chemistry
- Phenylbutazone / blood
- Phenylbutazone / pharmacology
- Phenylbutazone / therapeutic use
- Prostaglandin Antagonists / pharmacology
- Prostaglandin Antagonists / therapeutic use
- Random Allocation
- Tibia / pathology
- Tibia / physiology
Citations
This article has been cited 4 times.- Jacobs CC, Schnabel LV, McIlwraith CW, Blikslager AT. Non-steroidal anti-inflammatory drugs in equine orthopaedics. Equine Vet J 2022 Jan 25;54(4):636-48.
- Grzeskowiak RM, Schumacher J, Dhar MS, Harper DP, Mulon PY, Anderson DE. Bone and Cartilage Interfaces With Orthopedic Implants: A Literature Review. Front Surg 2020;7:601244.
- Richbourg HA, Mitchell CF, Gillett AN, McNulty MA. Tiludronate and clodronate do not affect bone structure or remodeling kinetics over a 60 day randomized trial. BMC Vet Res 2018 Mar 20;14(1):105.
- Viking Höglund O, Frendin J. Analgesic effect of meloxicam in canine acute dermatitis--a pilot study. Acta Vet Scand 2002;43(4):247-52.
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