Omneity® Pellets
All-In-One Vitamin & Mineral Pellet
Effects of phenylbutazone on gene expression of cyclooxygenase-1 and -2 in the oral, glandular gastric, and bladder mucosae of healthy horses.
Abstract: To assess gene expressions of cyclooxygenase-1 and -2 in oral, glandular gastric, and urinary bladder mucosae and determine the effect of oral administration of phenylbutazone on those gene expressions in horses. Methods: 12 healthy horses. Methods: Horses were allocated to receive phenylbutazone or placebo (6 horses/group); 1 placebo-treated horse with a cystic calculus was subsequently removed from the study, and those data were not analyzed. In each horse, the stomach and urinary bladder were evaluated for ulceration via endoscopy before and after experimental treatment. Oral, glandular gastric, and urinary bladder mucosa biopsy specimens were collected by use of a skin punch biopsy instrument (oral) or transendoscopically (stomach and bladder) before and after administration of phenylbutazone (4.4 mg/kg, p.o., q 12 h) in corn syrup or placebo (corn syrup alone) for 7 days. Cyclooxygenase-1 and -2 gene expressions were determined (via quantitative PCR techniques) in specimens collected before and after the 7-day treatment period and compared within and between groups. Prior to commencement of treatment, biopsy specimens from 7 horses were used to compare gene expressions among tissues. Results: The cyclooxygenase-1 gene was expressed in all tissues collected. The cyclooxygenase-2 gene was expressed in the glandular gastric and bladder mucosae but not in the oral mucosa. Cyclooxygenase gene expressions were unaffected by phenylbutazone administration. Conclusions: Cyclooxygenase-2 was constitutively expressed in glandular gastric and bladder mucosae but not in the oral mucosa of healthy horses. Oral administration of phenylbutazone at the maximum recommended dosage daily for 7 days did not affect cyclooxygenase-1 or -2 gene expression.
Publication Date: 2011-12-30 PubMed ID: 22204294DOI: 10.2460/ajvr.73.1.98Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research examined the impact of phenylbutazone (a type of medication) on certain gene expressions found in the oral, stomach, and bladder tissues of horses. The study found that while these genes were present in the horses’ tissues, the administration of phenylbutazone did not significantly affect their expression.
Methods
- This study involved 12 healthy horses that were split into groups. One group received phenylbutazone, while the other group received a placebo treatment. One horse in the placebo group was later removed from the study.
- The researchers carried out endoscopies on each horse to check for ulcers in the stomach and bladder before and after the experiment. This was done to ensure the tissues were healthy for the collection of biopsy specimens.
- Biopsy specimens were collected from the oral, stomach, and bladder tissues of the horses before and after administering phenylbutazone. This drug was mixed with corn syrup and given to the horses at a dosage of 4.4 mg/kg every 12 hours for a week. The placebo group received corn syrup alone.
- The gene expressions of cyclooxygenase-1 and cyclooxygenase-2 in the biopsy specimens were quantified using PCR techniques before and after the seven-day treatment. The researchers compared the results within and between the groups. Prior to the treatment, the researchers compared gene expressions among tissues from 7 horses.
Results
- All the tissues collected from the horses had expressions of the cyclooxygenase-1 gene. However, the cyclooxygenase-2 gene was only expressed in the stomach and bladder tissues, not in the oral tissues.
- The administration of phenylbutazone did not affect the expressions of either the cyclooxygenase-1 or cyclooxygenase-2 genes in the horses’ tissues.
Conclusions
- The cyclooxygenase-2 gene was naturally expressed in the stomach and bladder tissues, but not in the oral tissues of healthy horses.
- The administration of phenylbutazone, even at its maximum recommended dosage, daily for a week did not affect the expressions of the cyclooxygenase-1 or cyclooxygenase-2 genes in the horses’ tissues.
Cite This Article
APA
Nieto JE, Aleman M, Anderson JD, Fiack C, Snyder JR.
(2011).
Effects of phenylbutazone on gene expression of cyclooxygenase-1 and -2 in the oral, glandular gastric, and bladder mucosae of healthy horses.
Am J Vet Res, 73(1), 98-104.
https://doi.org/10.2460/ajvr.73.1.98 Publication
Researcher Affiliations
- Comparative Gastrointestinal Laboratory, Department of Surgical and Radiological Sciences, University of California-Davis, Davis, CA 95616, USA. jenieto@ucdavis.edu
MeSH Terms
- Animals
- Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
- Anti-Inflammatory Agents, Non-Steroidal / pharmacology
- Cyclooxygenase 1 / genetics
- Cyclooxygenase 2 / genetics
- Cystoscopy / veterinary
- Female
- Gastroscopy / veterinary
- Gene Expression Regulation / drug effects
- Horses / metabolism
- Male
- Mouth / drug effects
- Mouth / pathology
- Mucous Membrane / drug effects
- Mucous Membrane / pathology
- Phenylbutazone / administration & dosage
- Phenylbutazone / pharmacology
- Polymerase Chain Reaction / veterinary
- RNA, Messenger / metabolism
- Stomach / drug effects
- Stomach / pathology
- Urinary Bladder / drug effects
- Urinary Bladder / pathology
Citations
This article has been cited 3 times.- Tesena P, Vinijkumthorn R, Preuksathaporn T, Piyakul P, Chotikaprakal T, Sirireugwipas R, Wong-Aree K, Prapaiwan N. Evaluation of gastrointestinal tract lesions and serum malondialdehyde levels after repeated oral administration of phenylbutazone in horses. Vet Res Commun 2024 Aug;48(4):2343-2355.
- Donnell JR, Frisbie DD. Use of firocoxib for the treatment of equine osteoarthritis. Vet Med (Auckl) 2014;5:159-168.
- Martin LM, Johnson PJ, Amorim JR, DeClue AE. Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study. Int J Mol Sci 2020 Feb 20;21(4).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
