Effects of prior exercise on insulin-mediated and noninsulin-mediated glucose uptake in horses during a hyperglycaemic clamp.
Abstract: There is limited information about factors regulating glucose utilisation post exercise in horses. Objective: To determine the effects of a single bout of moderate intensity exercise on measures of insulin-mediated (IMGU) and noninsulin-mediated (NIMGU) glucose uptake during a hyperglycaemic clamp (HC). Methods: Hyperglycaemic clamps were administered in random order to 8 Standardbreds under 4 conditions: 1) rest, insulinopenia (R-L); 2) rest, hyperinsulinaemia (R-H); 3) post exercise (45 min at ∼ 50% VO2peak), insulinopenia (Ex-L) and 4) post exercise, hyperinsulinaemia (Ex-H). In the R-L and Ex-L trials, somatostatin was infused to suppress insulin secretion and induce insulinopenia. After 30 min, a 2 h HC was initiated with plasma glucose concentrations maintained at ∼ 10 mmol/l by variable glucose infusion. In R-H and Ex-H, regular insulin (1.0 mu/kg bwt/min) was also administered to induce physiological hyperinsulinaemia. Serum insulin and C-peptide concentrations were measured in samples obtained at 10 min intervals. Glucose uptake was calculated from mean glucose infusion rate (GIR) during the last 60 min of the HC. Results: In all HCs C-peptide remained below baseline concentrations, evidence of suppression of insulin secretion by somatostatin. Overall, mean ± s.e. insulin concentrations during the final 60 min of the HC in R-L and Ex-L were 5.7 ± 1.1 and 6.9 ± 1.9 mu/l respectively, and corresponding values in R-H and Ex-H were 64.1 ± 11.1 and 61.2 ± 10.9 mu/l. Prior exercise affected IMGU but not NIMGU. Over the final 60 min of the HC, mean GIR was higher (P < 0.001) in Ex-H (5.6 ± 1.1 mg/kg bwt/min) than in R-H (3.3 ± 0.9 mg/kg bwt/min), whereas mean GIR did not differ (P = 0.26) between R-L (1.2 ± 0.3 mg/kg bwt/min) and Ex-L (1.8 ± 0.5 mg/kg bwt/min). Conclusions: A single bout of moderate intensity exercise increased glucose uptake during a hyperglycaemic clamp under hyperinsulinaemic conditions but not under somatostatin-induced insulinopenia.
© 2010 EVJ Ltd.
Publication Date: 2011-05-27 PubMed ID: 21058994DOI: 10.1111/j.2042-3306.2010.00218.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study investigated how a single session of moderate-intensity exercise influences the uptake of glucose in horses, specifically looking at insulin-mediated and noninsulin-mediated glucose intake during a hyperglycaemic clamp. The research found that exercise did increase insulin-mediated glucose uptake under hyperglycaemic clamp conditions particularly with high insulin levels, but showed no change when insulin levels were suppressed by somatostatin.
Objective and Methodology of the Study
- The primary aim of the research was to examine how a single round of moderate-intensity exercise influences the insulin-mediated (IMGU) and noninsulin-mediated (NIMGU) glucose uptake during a hyperglycaemic clamp (HC), effectively monitoring how glucose is utilized post exercise in horses.
- To achieve this goal, hyperglycaemic clamps were carried out under four distinct conditions in 8 Standardbred horses. Conditions included rest with low insulin levels (R-L) and high insulin levels (R-H), and post-exercise with low insulin (Ex-L) and high insulin levels (Ex-H).
- Insulin suppression was achieved with the infusion of Somatostatin during R-L and Ex-L trials, inducing a state of insulinopenia. This was followed by the initiation of a 2-hour HC with plasma glucose concentrations maintained at around 10 mmol/l through variable glucose infusion.
- Further, in the R-H and Ex-H conditions, regular insulin was administered to induce a state of physiological hyperinsulinaemia. Serum insulin and C-peptide concentrations were measured in samples collected at 10-minute intervals.
- Finally, glucose uptake was calculated based on the mean glucose infusion rate (GIR) during the last 60 minutes of the HC.
Results of the Study
- The results indicated that somatostatin effectively suppressed insulin secretion in all HC trials as indicated by the fact that C-peptide concentrations remained below the baseline levels.
- It was observed that prior exercise impacted IMGU, but there was no apparent effect on NIMGU. Over the final 60 minutes of the HC, the mean GIR was higher in Ex-H when compared to R-H, indicating an increased glucose uptake following exercise under hyperinsulinaemia conditions.
- Conversely, the mean GIR did not vary significantly between R-L and Ex-L, indicating that exercise did not affect glucose uptake under insulinopenia (low insulin) conditions.
Conclusions of the Study
- The study concluded that a single session of moderate-intensity exercise led to an increase in glucose uptake during a hyperglycaemic clamp in hyperinsulinaemic conditions. However, this effect was not apparent under somatostatin-induced insulinopenia.
- This finding indicates that insulin availability plays a critical role in modulating the effects of exercise on glucose uptake in horses.
Cite This Article
APA
Geor RJ, Stewart-Hunt L, McCutcheon LJ.
(2011).
Effects of prior exercise on insulin-mediated and noninsulin-mediated glucose uptake in horses during a hyperglycaemic clamp.
Equine Vet J Suppl(38), 129-134.
https://doi.org/10.1111/j.2042-3306.2010.00218.x Publication
Researcher Affiliations
- Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Ontario, Canada. geor@cvm.msu.edu
MeSH Terms
- Animals
- Blood Glucose / metabolism
- Cross-Over Studies
- Female
- Glucose / metabolism
- Glucose Clamp Technique / veterinary
- Horses / blood
- Horses / metabolism
- Insulin / metabolism
- Male
- Physical Conditioning, Animal / physiology
Citations
This article has been cited 1 times.- Lindåse S, Nostell K, Söder J, Bröjer J. Relationship Between β-cell Response and Insulin Sensitivity in Horses based on the Oral Sugar Test and the Euglycemic Hyperinsulinemic Clamp. J Vet Intern Med 2017 Sep;31(5):1541-1550.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
