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Effects of prior exercise on insulin-mediated and noninsulin-mediated glucose uptake in horses during a hyperglycaemic clamp.
Abstract: There is limited information about factors regulating glucose utilisation post exercise in horses. Objective: To determine the effects of a single bout of moderate intensity exercise on measures of insulin-mediated (IMGU) and noninsulin-mediated (NIMGU) glucose uptake during a hyperglycaemic clamp (HC). Methods: Hyperglycaemic clamps were administered in random order to 8 Standardbreds under 4 conditions: 1) rest, insulinopenia (R-L); 2) rest, hyperinsulinaemia (R-H); 3) post exercise (45 min at ∼ 50% VO2peak), insulinopenia (Ex-L) and 4) post exercise, hyperinsulinaemia (Ex-H). In the R-L and Ex-L trials, somatostatin was infused to suppress insulin secretion and induce insulinopenia. After 30 min, a 2 h HC was initiated with plasma glucose concentrations maintained at ∼ 10 mmol/l by variable glucose infusion. In R-H and Ex-H, regular insulin (1.0 mu/kg bwt/min) was also administered to induce physiological hyperinsulinaemia. Serum insulin and C-peptide concentrations were measured in samples obtained at 10 min intervals. Glucose uptake was calculated from mean glucose infusion rate (GIR) during the last 60 min of the HC. Results: In all HCs C-peptide remained below baseline concentrations, evidence of suppression of insulin secretion by somatostatin. Overall, mean ± s.e. insulin concentrations during the final 60 min of the HC in R-L and Ex-L were 5.7 ± 1.1 and 6.9 ± 1.9 mu/l respectively, and corresponding values in R-H and Ex-H were 64.1 ± 11.1 and 61.2 ± 10.9 mu/l. Prior exercise affected IMGU but not NIMGU. Over the final 60 min of the HC, mean GIR was higher (P < 0.001) in Ex-H (5.6 ± 1.1 mg/kg bwt/min) than in R-H (3.3 ± 0.9 mg/kg bwt/min), whereas mean GIR did not differ (P = 0.26) between R-L (1.2 ± 0.3 mg/kg bwt/min) and Ex-L (1.8 ± 0.5 mg/kg bwt/min). Conclusions: A single bout of moderate intensity exercise increased glucose uptake during a hyperglycaemic clamp under hyperinsulinaemic conditions but not under somatostatin-induced insulinopenia.
© 2010 EVJ Ltd.
Publication Date: 2011-05-27 PubMed ID: 21058994DOI: 10.1111/j.2042-3306.2010.00218.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
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The study investigated how a single session of moderate-intensity exercise influences the uptake of glucose in horses, specifically looking at insulin-mediated and noninsulin-mediated glucose intake during a hyperglycaemic clamp. The research found that exercise did increase insulin-mediated glucose uptake under hyperglycaemic clamp conditions particularly with high insulin levels, but showed no change when insulin levels were suppressed by somatostatin.
Objective and Methodology of the Study
- The primary aim of the research was to examine how a single round of moderate-intensity exercise influences the insulin-mediated (IMGU) and noninsulin-mediated (NIMGU) glucose uptake during a hyperglycaemic clamp (HC), effectively monitoring how glucose is utilized post exercise in horses.
- To achieve this goal, hyperglycaemic clamps were carried out under four distinct conditions in 8 Standardbred horses. Conditions included rest with low insulin levels (R-L) and high insulin levels (R-H), and post-exercise with low insulin (Ex-L) and high insulin levels (Ex-H).
- Insulin suppression was achieved with the infusion of Somatostatin during R-L and Ex-L trials, inducing a state of insulinopenia. This was followed by the initiation of a 2-hour HC with plasma glucose concentrations maintained at around 10 mmol/l through variable glucose infusion.
- Further, in the R-H and Ex-H conditions, regular insulin was administered to induce a state of physiological hyperinsulinaemia. Serum insulin and C-peptide concentrations were measured in samples collected at 10-minute intervals.
- Finally, glucose uptake was calculated based on the mean glucose infusion rate (GIR) during the last 60 minutes of the HC.
Results of the Study
- The results indicated that somatostatin effectively suppressed insulin secretion in all HC trials as indicated by the fact that C-peptide concentrations remained below the baseline levels.
- It was observed that prior exercise impacted IMGU, but there was no apparent effect on NIMGU. Over the final 60 minutes of the HC, the mean GIR was higher in Ex-H when compared to R-H, indicating an increased glucose uptake following exercise under hyperinsulinaemia conditions.
- Conversely, the mean GIR did not vary significantly between R-L and Ex-L, indicating that exercise did not affect glucose uptake under insulinopenia (low insulin) conditions.
Conclusions of the Study
- The study concluded that a single session of moderate-intensity exercise led to an increase in glucose uptake during a hyperglycaemic clamp in hyperinsulinaemic conditions. However, this effect was not apparent under somatostatin-induced insulinopenia.
- This finding indicates that insulin availability plays a critical role in modulating the effects of exercise on glucose uptake in horses.
Cite This Article
APA
Geor RJ, Stewart-Hunt L, McCutcheon LJ.
(2011).
Effects of prior exercise on insulin-mediated and noninsulin-mediated glucose uptake in horses during a hyperglycaemic clamp.
Equine Vet J Suppl(38), 129-134.
https://doi.org/10.1111/j.2042-3306.2010.00218.x Publication
Researcher Affiliations
- Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Ontario, Canada. geor@cvm.msu.edu
MeSH Terms
- Animals
- Blood Glucose / metabolism
- Cross-Over Studies
- Female
- Glucose / metabolism
- Glucose Clamp Technique / veterinary
- Horses / blood
- Horses / metabolism
- Insulin / metabolism
- Male
- Physical Conditioning, Animal / physiology
Citations
This article has been cited 1 times.- Lindåse S, Nostell K, Söder J, Bröjer J. Relationship Between β-cell Response and Insulin Sensitivity in Horses based on the Oral Sugar Test and the Euglycemic Hyperinsulinemic Clamp. J Vet Intern Med 2017 Sep;31(5):1541-1550.
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