Effects of roscovitine on maintenance of the germinal vesicle in horse oocytes, subsequent nuclear maturation, and cleavage rates after intracytoplasmic sperm injection.
Abstract: This study was conducted to evaluate the effects of roscovitine on suppression of meiosis, subsequent meiotic maturation, and cleavage rates after intracytoplasmic sperm injection of horse oocytes. Oocytes were classified as having compact or expanded cumuli (Com or Exp oocytes) and were divided into three culture groups: 30 h culture in maturation medium (30 h Mat); 54 h culture in maturation medium (54 h Mat), or 24 h culture in medium containing 66 micro mol roscovitine l(-1) and then 30 h culture in maturation medium (Ros+M). After maturation, oocytes were subjected to intracytoplasmic sperm injection and cultured in G1.2 medium for 96 h. Among oocytes fixed immediately after roscovitine culture, 26 of 31 (84%) Com oocytes and 16 of 28 (57%) Exp oocytes were at the germinal vesicle stage (P<0.05). After maturation culture, there were no differences in maturation rates or morphological cleavage rates among treatments. Among Com oocytes, significantly more embryos in the Ros+M treatment than in the 54 h Mat treatment had cleaved with > or = two normal nuclei (63 versus 36%; P<0.05); whereas among Exp oocytes, significantly more embryos in the 30 h Mat treatment than in the Ros+M treatment (63 versus 42%; P<0.05) had cleaved with > or = two normal nuclei. The average number of nuclei in embryos at 96 h was significantly higher (P<0.05) in Ros+M Com oocytes (13.5) than in any other Com or Exp group. These results demonstrate that roscovitine can reversibly maintain equine oocytes in the germinal vesicle stage for up to 24 h, and that such suppression may increase the developmental potential of Com, but not Exp, oocytes.
Publication Date: 2003-04-26 PubMed ID: 12713432
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the impact of a chemical called roscovitine on the maturation and fertilization process of horse oocytes (eggs), proving that it can delay maturation and possibly enhance the developmental potential of certain types of oocytes.
Objective of the Study
- The study aimed at understanding the effects of roscovitine on horse oocytes, specifically looking at its impact on the suppression of meiosis, subsequent meiotic maturation, and cleavage rates following an intracytoplasmic sperm injection (ICSI).
Methodology Used
- The study utilized horse oocytes that were classified into two types based on the condition of their cumuli: compact cumuli (Com) and expanded cumuli (Exp).
- These oocytes were divided into three culture groups: 30 hours in maturation medium (labeled 30 h Mat), 54 hours in maturation medium (54 h Mat), and 24 hours in medium containing 66 micro mol roscovitine l(-1) followed by 30 hours in maturation medium (Ros+M).
- Following maturation, the oocytes were subjected to ICSI and then cultured in G1.2 medium for a further 96 hours.
Study Findings
- Upon examination after roscovitine culture, 84% of Com oocytes and 57% of Exp oocytes were found in the germinal vesicle stage.
- No significant difference was observed in the maturation and morphological cleavage rates amongst the three treatment groups.
- Among the Com oocytes, a significant increase in the number of embryos with two or more normal nuclei was observed in the Ros+M treatment group compared to the 54h Mat group.
- Among the Exp oocytes, however, more embryos in the 30h Mat treatment group exhibited two or more normal nuclei compared to those in the Ros+M group.
- The average number of nuclei in embryos at 96 hours was significantly higher in Ros+M Com oocytes than in any other Com or Exp group.
- The results demonstrate that roscovitine can preserve horse oocytes in the germinal vesicle stage for up to 24 hours, and this suppression may potentially enhance the developmental potential of Com oocytes.
Cite This Article
APA
Franz LC, Choi YH, Squires EL, Seidel GE, Hinrichs K.
(2003).
Effects of roscovitine on maintenance of the germinal vesicle in horse oocytes, subsequent nuclear maturation, and cleavage rates after intracytoplasmic sperm injection.
Reproduction, 125(5), 693-700.
Publication
Researcher Affiliations
- Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, Colorado 80523, USA.
MeSH Terms
- Animals
- Chromatin / ultrastructure
- Enzyme Inhibitors / pharmacology
- Female
- Fertilization in Vitro
- Horses
- Maturation-Promoting Factor / antagonists & inhibitors
- Oocytes / cytology
- Oocytes / drug effects
- Oogenesis / drug effects
- Purines / pharmacology
- Roscovitine
- Sperm Injections, Intracytoplasmic
Citations
This article has been cited 1 times.- Pandey S, Somal A, Parmar MS, Gupta S, Bharti MK, Bhat IA, Indu B, Chandra V, Kumar GS, Sharma GT. Effect of roscovitine on developmental competence of small follicle-derived buffalo oocytes. Indian J Med Res 2018 Dec;148(Suppl):S140-S150.
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