Effects of the Glucosinolate Sinigrin in Combination With a Noniodine Supplemented Diet on Serum Iodine and Thyroid Hormone Concentrations in Nonpregnant Mares.
Abstract: Exposure to plants containing glucosinolates (GSLs) affects thyroid function in many species, in horses is implicated in the birth of foals with congenital hypothyroidism. The present study was performed to determine the effect of feeding a GSL (sinigrin) in combination with a low-iodine diet for 12 weeks on thyroid hormones and serum iodine concentrations in nonpregnant mares. Nineteen mares aged 2-14 years were divided into control (n = 6), low (20 mmol/day) (n = 7) and high GSL (35 mmol/day) (n = 6) groups. Thyrotropin-releasing hormone (TRH) stimulation tests and serum iodine measurements were performed at 0 and 12 weeks. Total triiodothyronine (TT3), total thyroxine (TT4), and thyroid-stimulating hormone (TSH) concentrations were measured at the baseline and in post-TRH samples. The post-TRH value minus the basal value (Delta Δ) and fold change (FC) were calculated for TSH, TT3, and TT4. Data were analyzed at P < .05. Highlights included Delta Δ and FC TT4 and TT3 concentrations having a group and week interaction (P < .001) with week 12 control mares having higher values than mares in week 12 low and high GSL groups. TT4 FC values had a group (P < .001) and group by week interaction (P < .001) with week 12 control concentrations higher (P < .006) than all groups. Iodine concentrations decreased (P < .002) over time in GSL mares. In conclusion, feeding mares a low-iodine diet with 20 and 35 mmol sinigrin/day resulted in lower serum iodine concentrations.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication Date: 2020-04-25 PubMed ID: 32684255DOI: 10.1016/j.jevs.2020.103110Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research examined how the combination of a type of glucosinolate known as sinigrin and a non-iodine supplemented diet impact the thyroid hormones and serum iodine levels in non-pregnant mares over a period of 12 weeks. The study found this combination resulted in lower iodine levels in the horses’ serum, an indicator that their thyroid function could be affected.
Study Setup and Participants
- The study was conducted on nineteen non-pregnant mares that were between 2 to 14 years.
- The mares were divided into three groups: a control group with 6 mares, a low(sinigrin) group with 7 mares that received 20 mmol of sinigrin each day, and a high(sinigrin) group with 6 mares that took 35 mmol of sinigrin daily.
Examinations Over Time
- The research included Thyrotropin-releasing hormone (TRH) stimulation tests and serum iodine measurements, which were conducted at the start and end (12th week) of the study.
- The concentrations of Total triiodothyronine (TT3), total thyroxine (TT4), and thyroid-stimulating hormone (TSH) were measured—both at the start and in the post-TRH samples.
Analyses and Comparisons
- The researchers calculated the difference between the post-TRH value and the baseline (referred to as Delta Δ) and fold change (FC) for TSH, TT3, and TT4. These computations provide insights into the degree of change in thyroid hormone levels as a result of the sinigrin-intake and the low-iodine diet.
- The data was analyzed, with findings being significant at a level of p<.05.
Findings
- The study found that both FC and Delta Δ TT4 and TT3 concentrations had a group and week interaction (p<.001), indicating that the quantities of these thyroid hormones varied between groups and weeks. The control group had higher values than both the low and high sinigrin groups in the 12th week.
- Similar results were observed for TT4 FC values, with the control group having more elevated hormone concentrations than the others at week 12 (p<.006).
- Iodine concentrations were found to have decreased (p<.002) over the study period in mares taking sinigrin.
Conclusion
- The study concluded that feeding mares a low-iodine diet along with either 20 or 35 mmol of sinigrin per day led to a decrease in serum iodine concentrations, hinting that this combination may impact the mares’ thyroid function.
Cite This Article
APA
Lopez-Rodriguez MF, Cymbaluk N, Epp T, Laarveld B, Serrano Recalde EC, Simko E, Card C.
(2020).
Effects of the Glucosinolate Sinigrin in Combination With a Noniodine Supplemented Diet on Serum Iodine and Thyroid Hormone Concentrations in Nonpregnant Mares.
J Equine Vet Sci, 91, 103110.
https://doi.org/10.1016/j.jevs.2020.103110 Publication
Researcher Affiliations
- Department Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
- Department Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
- Department Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
- Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
- Department Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
- Department of Veterinary Pathology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
- Department Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. Electronic address: claire.card@usask.ca.
MeSH Terms
- Animals
- Diet
- Female
- Glucosinolates
- Horses
- Iodine
- Thyroid Hormones
Citations
This article has been cited 4 times.- Ellero N, Lanci A, Baldassarro VA, Alastra G, Mariella J, Cescatti M, Castagnetti C, Giardino L. Study on NGF and VEGF during the Equine Perinatal Period-Part 2: Foals Affected by Neonatal Encephalopathy.. Vet Sci 2022 Aug 26;9(9).
- Ellero N, Lanci A, Baldassarro VA, Alastra G, Mariella J, Cescatti M, Giardino L, Castagnetti C. Study on NGF and VEGF during the Equine Perinatal Period-Part 1: Healthy Foals Born from Normal Pregnancy and Parturition.. Vet Sci 2022 Aug 23;9(9).
- Li X, Wang P, Wang J, Wang H, Liu T, Zhang X, Song J, Yang W, Wu C, Yang H, Liu L, Li X. A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots.. Int J Mol Sci 2022 Mar 9;23(6).
- Sun X. Invited Review: Glucosinolates Might Result in Low Methane Emissions From Ruminants Fed Brassica Forages.. Front Vet Sci 2020;7:588051.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
