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Home / Equine Research Bank / Vet J / Efficacy of imidocarb dipropionate in eliminating Theileria ...
Veterinary journal (London, England : 1997)2012; 196(3); 541-546; doi: 10.1016/j.tvjl.2012.10.025

Efficacy of imidocarb dipropionate in eliminating Theileria equi from experimentally infected horses.

Abstract: Theileria equi, one of the causative agents of equine piroplasmosis, is endemic in many regions of the world but is considered a 'foreign' animal disease in the USA. In an effort to prevent the importation of T. equi, stringent serological screening of horses is practiced prior to entry to the USA. Current regulatory options available where horses are found to be infected include permanent quarantine with or without chemotherapy, repatriation, or euthanasia. Chemotherapeutics that eliminate infection and subsequently transmission risk are critical in the management of infected horses. In this study, the efficacy of the drug imidocarb dipropionate against experimental T. equi infection was assessed. Of nine horses experimentally inoculated with T. equi isolated from an animal previously imported from Peru, six were treated with imidocarb dipropionate after the resolution of the acute phase of the disease. Elimination of the parasite was demonstrated in 5/6 by nested PCR, blood transfusions to naïve horses, and reversion to seronegative status. The findings support the use of this drug as a potential treatment option in controlling outbreaks of T. equi, and also suggest that 'combination testing' using both serological and PCR detection methods are necessary to demonstrate clearance of infection.
Published by Elsevier Ltd.
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Publication Date: 2012-11-28 PubMed ID: 23199699DOI: 10.1016/j.tvjl.2012.10.025Google Scholar: Lookup
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  • Controlled Clinical Trial
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study examines the effectiveness of the drug imidocarb dipropionate in dealing with Theileria equi infections in horses. It concludes that imidocarb dipropionate successfully eliminated the parasite in a majority of the tested horses and suggests its use as a potential treatment option.

Objective of the Study

  • The objective of this study was to assess the efficiency of the chemotherapeutic drug, imidocarb dipropionate, in managing horses that were experimentally infected with Theileria equi – an agent causing equine piroplasmosis.

Why was the Study Performed?

  • The study was conducted because T. equi is considered a ‘foreign’ animal disease in the USA. So, to prevent its importation into the country, horses undergo strict serological screening prior to entry.
  • In cases where horses are diagnosed with the infection, current regulatory solutions include permanent quarantine with or without chemotherapy, repatriation, or euthanasia.
  • Chemotherapeutics that can successfully eliminate the infection are therefore critical in managing infected horses, making research in this area highly important.

Methodology of the Research

  • In this study, nine horses were inoculated or infected with T. equi, isolated from an animal previously imported from Peru.
  • Six of these horses were subject to treatment with imidocarb dipropionate after the acute phase of the disease had passed.

Results of the Investigation

  • The study found that the parasite was eliminated in five out of six treated horses.
  • The clearance of the infection was confirmed by nested PCR, blood transfusions to naive horses, and reversion to seronegative status.

Conclusions Drawn

  • The findings support the usage of imidocarb dipropionate as a potential treatment option in controlling outbreaks of T. equi among horses.
  • The research also proposed that ‘combination testing’ which uses both serological and PCR detection methods are required to demonstrate the successful clearance of the infection.

Cite This Article

APA
Grause JF, Ueti MW, Nelson JT, Knowles DP, Kappmeyer LS, Bunn TO. (2012). Efficacy of imidocarb dipropionate in eliminating Theileria equi from experimentally infected horses. Vet J, 196(3), 541-546. https://doi.org/10.1016/j.tvjl.2012.10.025

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 196
Issue: 3
Pages: 541-546

Researcher Affiliations

Grause, Juanita F
  • Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA 50010, USA. juanita.f.grause@aphis.usda.gov
Ueti, Massaro W
    Nelson, Jeffrey T
      Knowles, Donald P
        Kappmeyer, Lowell S
          Bunn, Thomas O

            MeSH Terms

            • Animals
            • Antiprotozoal Agents / therapeutic use
            • Horse Diseases / drug therapy
            • Horse Diseases / parasitology
            • Horses
            • Imidocarb / analogs & derivatives
            • Imidocarb / therapeutic use
            • Theileria / classification
            • Theileriasis / drug therapy

            Citations

            This article has been cited 23 times.
            1. Firat R, Ulucesme MC, Eyvaz A, Alatas M, Aktas M, Ceylan O, Sevinc F, Ozubek S. Persistence and Transmission Dynamics of Babesia ovis After Imidocarb Dipropionate Treatment: Evaluation via Blood Transfusion and Tick Infestation. Pathogens 2025 Dec 20;15(1).
              doi: 10.3390/pathogens15010007pubmed: 41598990google scholar: lookup
            2. Cardillo NN, Villarino NF, Kappmeyer LS, Chung CJ, Suarez CE, Bastos RG. Tafenoquine succinate inhibits the growth of the equine piroplasmosis hemoparasites Theileria equi and Babesia caballi. Parasit Vectors 2026 Jan 27;19(1):61.
              doi: 10.1186/s13071-026-07262-ypubmed: 41593688google scholar: lookup
            3. Liang Q, Li X, Zhang X, Zhang Y, Wang J, Liu Z, Ye Y, Bai Y, Zhao S, Luo J, You C, Yin H, Guan G. Furamidine, a methyltransferase inhibitor, is a potential anti-Babesia spp. chemotherapeutic. Parasitol Res 2025 Dec 3;124(12):149.
              doi: 10.1007/s00436-025-08576-zpubmed: 41339613google scholar: lookup
            4. Ullah A, Geng M, Chen W, Zhu Q, Shi L, Zhang X, Akhtar MF, Wang C, Khan MZ. Effect of Parasitic Infections on Hematological Profile, Reproductive and Productive Performance in Equines. Animals (Basel) 2025 Nov 14;15(22).
              doi: 10.3390/ani15223294pubmed: 41302002google scholar: lookup
            5. Poh KC, Oyen K, Onzere CK, Kappmeyer LS, Bastos RG. Haemaphysalis longicornis ticks are unable to transstadially transmit Theileria haneyi to horses. Front Vet Sci 2025;12:1572944.
              doi: 10.3389/fvets.2025.1572944pubmed: 40241805google scholar: lookup
            6. Axt CW, Springer A, von Luckner J, Naucke TJ, Müller E, Strube C, Schäfer I. [Equine piroplasmosis: Case descriptions and overview of the epidemiological situation in Europe with focus on Germany]. Tierarztl Prax Ausg G Grosstiere Nutztiere 2025 Feb;53(1):49-58.
              doi: 10.1055/a-2457-5516pubmed: 39631762google scholar: lookup
            7. Mendoza FJ, Pérez-Écija A, Kappmeyer LS, Suarez CE, Bastos RG. New insights in the diagnosis and treatment of equine piroplasmosis: pitfalls, idiosyncrasies, and myths. Front Vet Sci 2024;11:1459989.
              doi: 10.3389/fvets.2024.1459989pubmed: 39205808google scholar: lookup
            8. Onzere CK, Hulbert M, Sears KP, Williams LBA, Fry LM. Tulathromycin and Diclazuril Lack Efficacy against Theileria haneyi, but Tulathromycin Is Not Associated with Adverse Clinical Effects in Six Treated Adult Horses. Pathogens 2023 Mar 14;12(3).
              doi: 10.3390/pathogens12030453pubmed: 36986375google scholar: lookup
            9. Graham H, van Kalsbeek P, van der Goot J, Koene MGJ. Low seroprevalence of equine piroplasmosis in horses exported from the Netherlands between 2015 and 2021. Front Vet Sci 2022;9:954046.
              doi: 10.3389/fvets.2022.954046pubmed: 36299627google scholar: lookup
            10. Coultous RM, Sutton DGM, Boden LA. A risk assessment of equine piroplasmosis entry, exposure and consequences in the UK. Equine Vet J 2023 Mar;55(2):282-294.
              doi: 10.1111/evj.13579pubmed: 35478189google scholar: lookup
            11. Salinas-Estrella E, Ueti MW, Lobanov VA, Castillo-Payró E, Lizcano-Mata A, Badilla C, Martínez-Ibáñez F, Mosqueda J. Serological and molecular detection of Babesia caballi and Theileria equi in Mexico: A prospective study. PLoS One 2022;17(3):e0264998.
              doi: 10.1371/journal.pone.0264998pubmed: 35259206google scholar: lookup
            12. Nadal C, Marsot M, Le Metayer G, Boireau P, Guillot J, Bonnet SI. Spatial and Temporal Circulation of Babesia caballi and Theileria equi in France Based on Seven Years of Serological Data. Pathogens 2022 Feb 9;11(2).
              doi: 10.3390/pathogens11020227pubmed: 35215171google scholar: lookup
            13. Schnittger L, Ganzinelli S, Bhoora R, Omondi D, Nijhof AM, Florin-Christensen M. The Piroplasmida Babesia, Cytauxzoon, and Theileria in farm and companion animals: species compilation, molecular phylogeny, and evolutionary insights. Parasitol Res 2022 May;121(5):1207-1245.
              doi: 10.1007/s00436-022-07424-8pubmed: 35098377google scholar: lookup
            14. Idoko IS, Edeh RE, Adamu AM, Machunga-Mambula S, Okubanjo OO, Balogun EO, Adamu S, Johnson W, Kappmeyer L, Mousel M, Ueti MW. Molecular and Serological Detection of Piroplasms in Horses from Nigeria. Pathogens 2021 Apr 23;10(5).
              doi: 10.3390/pathogens10050508pubmed: 33922468google scholar: lookup
            15. Nardini R, Bartolomé Del Pino LE, Cersini A, Manna G, Viola MR, Antognetti V, Autorino GL, Scicluna MT. Comparison of PCR-based methods for the detection of Babesia caballi and Theileria equi in field samples collected in Central Italy. Parasitol Res 2021 Jun;120(6):2157-2164.
              doi: 10.1007/s00436-021-07153-4pubmed: 33855619google scholar: lookup
            16. Sears K, Knowles D, Dinkel K, Mshelia PW, Onzere C, Silva M, Fry L. Imidocarb Dipropionate Lacks Efficacy against Theileria haneyi and Fails to Consistently Clear Theileria equi in Horses Co-Infected with T. haneyi. Pathogens 2020 Dec 10;9(12).
              doi: 10.3390/pathogens9121035pubmed: 33321715google scholar: lookup
            17. Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny. Pathogens 2020 Nov 8;9(11).
              doi: 10.3390/pathogens9110926pubmed: 33171698google scholar: lookup
            18. Lobanov VA, Peckle M, Massard CL, Brad Scandrett W, Gajadhar AA. Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis. Parasit Vectors 2018 Mar 2;11(1):125.
              doi: 10.1186/s13071-018-2751-6pubmed: 29499748google scholar: lookup
            19. Gimenez F, Hines SA, Evanoff R, Ojo KK, Van Voorhis WC, Maly DJ, Vidadala RSR, Mealey RH. In vitro growth inhibition of Theileria equi by bumped kinase inhibitors. Vet Parasitol 2018 Feb 15;251:90-94.
              doi: 10.1016/j.vetpar.2017.12.024pubmed: 29426483google scholar: lookup
            20. Van Voorhis WC, Doggett JS, Parsons M, Hulverson MA, Choi R, Arnold SLM, Riggs MW, Hemphill A, Howe DK, Mealey RH, Lau AOT, Merritt EA, Maly DJ, Fan E, Ojo KK. Extended-spectrum antiprotozoal bumped kinase inhibitors: A review. Exp Parasitol 2017 Sep;180:71-83.
              doi: 10.1016/j.exppara.2017.01.001pubmed: 28065755google scholar: lookup
            21. Hines SA, Ramsay JD, Kappmeyer LS, Lau AO, Ojo KK, Van Voorhis WC, Knowles DP, Mealey RH. Theileria equi isolates vary in susceptibility to imidocarb dipropionate but demonstrate uniform in vitro susceptibility to a bumped kinase inhibitor. Parasit Vectors 2015 Jan 20;8:33.
              doi: 10.1186/s13071-014-0611-6pubmed: 25600252google scholar: lookup
            22. Guidi E, Pradier S, Lebert I, Leblond A. Piroplasmosis in an endemic area: analysis of the risk factors and their implications in the control of Theileriosis and Babesiosis in horses. Parasitol Res 2015 Jan;114(1):71-83.
              doi: 10.1007/s00436-014-4161-9pubmed: 25280516google scholar: lookup
            23. Awinda PO, Mealey RH, Williams LB, Conrad PA, Packham AE, Reif KE, Grause JF, Pelzel-McCluskey AM, Chung C, Bastos RG, Kappmeyer LS, Howe DK, Ness SL, Knowles DP, Ueti MW. Serum antibodies from a subset of horses positive for Babesia caballi by competitive enzyme-linked immunosorbent assay demonstrate a protein recognition pattern that is not consistent with infection. Clin Vaccine Immunol 2013 Nov;20(11):1752-7.
              doi: 10.1128/CVI.00479-13pubmed: 24049108google scholar: lookup
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