Efficiency of embryonic development after intrafollicular and intraoviductal transfer of in vitro and in vivo matured horse oocytes.
Abstract: In vivo techniques, such as intraoviductal oocyte transfer (OT) and intrafollicular oocyte transfer (IFOT), can be considered as alternatives to bypass the lack of efficient superovulation treatments and the inadequacy of conventional in vitro fertilization techniques in the horse. We compared embryo production after transfer of in vivo recovered oocytes (1) into a recipient's oviduct or (2) into her preovulatory follicle either immediately after ovum pick-up or (3) after in vitro maturation (IVM). Recipients were inseminated with fresh semen of a stallion with a known normal fertility. Ten days after surgery, rates of embryos collected in excess to the number of ovulations were calculated and compared for each group. Embryo collection rates were 32.5% (13 of 40), 5.5% (3 of 55), and 12.8% (6 of 47) for OT, post-IVM IFOT, and immediate IFOT, respectively. Oocyte transfer significantly yielded more embryos than did immediate IFOT and post-IVM IFOT. We also showed that in vitro matured oocytes could successfully be used for IFOT. Our results also suggest that improvement of the IFOT technique could turn it into an inexpensive and easy-to-perform procedure that could be an answer to the inefficiency of superovulation treatments in the mare.
Publication Date: 2009-03-31 PubMed ID: 19339039DOI: 10.1016/j.theriogenology.2009.02.001Google Scholar: Lookup
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- Journal Article
Summary
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The research article assesses the efficiency of embryonic development in horses through the use of varying transfer techniques. The study supports the use of in vivo techniques like intraoviductal oocyte transfer (OT) and intrafollicular oocyte transfer (IFOT) as potential solutions to shortcomings of conventional fertility treatments in horses.
Objective of the Research
- The core objective of this experiment was to examine if in vivo techniques such as intraoviductal oocyte transfer (OT) and intrafollicular oocyte transfer (IFOT) could be used as effective replacements for deficient superovulation treatments and in vitro fertilization techniques in horses.
Methodology
- The study compared embryo production after an in vivo recovered oocyte was transferred into a recipient’s oviduct or her preovulatory follicle, either immediately after ovum pick-up or after in vitro maturation (IVM).
- Fresh semen of a stallion with known normal fertility was inseminated into the recipients.
- Ten days post-surgery, the rates of embryos collected in excess of the number of ovulations were calculated and compared for each group.
Results
- The embryo collection rates were 32.5% for OT, 5.5% for post-IVM IFOT and 12.8% for immediate IFOT.
- Oocyte transfer significantly yielded more embryos than did immediate IFOT and post-IVM IFOT.
- The study also demonstrated that in vitro matured oocytes can effectively be utilized for IFOT.
Conclusion
- The results suggest that enhancement of the IFOT technique could transform it into an affordable and simple process that could act as a solution to the inefficiency of superovulation treatments in mares.
Cite This Article
APA
Deleuze S, Goudet G, Caillaud M, Lahuec C, Duchamp G.
(2009).
Efficiency of embryonic development after intrafollicular and intraoviductal transfer of in vitro and in vivo matured horse oocytes.
Theriogenology, 72(2), 203-209.
https://doi.org/10.1016/j.theriogenology.2009.02.001 Publication
Researcher Affiliations
- Faculté de Médecine Vétérinaire, Département des Sciences Cliniques-Clinique Equine, Université de Liège, Liège, Belgium. S.Deleuze@ulg.ac.be
MeSH Terms
- Animals
- Cells, Cultured
- Embryonic Development / physiology
- Fallopian Tubes
- Female
- Horses / embryology
- Insemination, Artificial / veterinary
- Male
- Oocytes / growth & development
- Oocytes / transplantation
- Ovarian Follicle
- Reproductive Techniques / veterinary
- Tissue and Organ Harvesting / veterinary
Citations
This article has been cited 5 times.- Teng M, Zhao M, Mu B, Lei A. Allogenic Follicular Fosterage Technology: Problems, Progress and Potential. Vet Sci 2024 Jun 17;11(6).
- Martinez de Andino EV, Brom-de-Luna JG, Canesin HS, Rader K, Resende HL, Ripley AM, Love CC, Hinrichs K. Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer. J Assist Reprod Genet 2019 Jun;36(6):1237-1250.
- Khalili MA, Dehghan M, Nazari S, Agha-Rahimi A. Assessment of ovarian tissues autografted to various body sites followed by IVM in mouse. Iran J Reprod Med 2014 Mar;12(3):199-204.
- Jiménez-Trigos E, Vicente JS, Marco-Jiménez F. Live birth from slow-frozen rabbit oocytes after in vivo fertilisation. PLoS One 2013;8(12):e83399.
- Mugnier S, Kervella M, Douet C, Canepa S, Pascal G, Deleuze S, Duchamp G, Monget P, Goudet G. The secretions of oviduct epithelial cells increase the equine in vitro fertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?. Reprod Biol Endocrinol 2009 Nov 19;7:129.
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