Electrochemotherapy of horses. A preliminary clinical report.
Abstract: Sarcoids are skin spontaneous tumours detected in horses. It can be cured by chemotherapy by using cisplatin. A multisequence treatment must be performed. Problems are present due to the poor diffusion of the hydrophilic product in the tumours. Electropulsation is known to drastically enhance the effect of antitumoral drugs in vivo. Taking into account the very successful results of the group in Ljubljana (Slovenia), we started a research clinical program where electropulsation was applied after local cisplatin injection. The size of sarcoids is large (several centimeters). A specially designed set of wire contact electrodes was built. The distance between the electrodes was 0.9 cm and their length was 0.9 cm. The contact with the skin was obtained by a conductive paste. A PS15 Jouan Electropulsator was used to deliver eight pulses of 0.1 ms at a 1-Hz frequency with a 1.3-kV voltage. The animal was anesthesized. Intratumoral cisplatin injections were operated every 0.6 cm (0.2 ml at a 1-mg/ml concentration). Five minutes after the first drug injection, multiple electrotreatments were applied by moving the electrodes between the pulse applications. This allows the treatment of all the tumour surface. Several successive treatments were performed with a delay of 2 weeks between each. All lesions completely responded. The sarcoids disappear after only 2 or 3 electrochemotherapies. Objective responses were obtained in 100% of the treated lesions. All horses tolerated the treatment well. No adverse effect from the electric pulses was observed even in the case of a high number of pulses, or when several consecutive treatments were applied. No regrowth was observed in the 18 months follow-up period.
Publication Date: 2002-01-12 PubMed ID: 11786351DOI: 10.1016/s1567-5394(01)00156-6Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research presents a preliminary clinical report detailing the successful use of electrochemotherapy, combining the use of electrical pulses and the drug cisplatin, to treat spontaneous skin tumours (sarcoids) in horses.
Background and Objective of the Study
- The study focuses on the treatment of sarcoids, which are common skin tumours found in horses. Though chemotherapy using the drug cisplatin can treat sarcoids, the process often presents challenges due to the poor diffusion of the hydrophilic (water-attracting) product within the tumours.
- The researchers aim to enhance the effectiveness of the antitumoral drug treatment with the use of electropulsation, a technique known to significantly boost the impact of such drugs in live organisms (in vivo).
Methodology
- A clinical research program was initiated where electropulsation was employed post local injection of cisplatin into the tumour. This methodology was inspired by the successful results achieved by a group in Ljubljana, Slovenia.
- Given the large size of sarcoids, a specific set of wire contact electrodes were created, ensuring appropriate distance and length for effective treatment. A conductive paste was used to establish contact with the skin.
- An Electropulsator was used to produce eight pulses of 0.1 milliseconds at a frequency of 1 Hz and a voltage of 1.3 kV. The horses were anesthetized during the treatment.
- Cisplatin was intratumorally injected every 0.6 cm. Five minutes following the initial drug injection, multiple electro-treatments were administered by shifting the electrodes between pulse applications. This enabled the treatment to cover the entire tumour surface.
- Several concurrent treatments were performed with two-week intervals.
Results and Conclusion
- The findings of the study reveal that all treated lesions responded completely to the treatment. The tumours disappeared after two or three instances of electrochemotherapy. A favourable response was observed in 100% of the treated lesions.
- All the horses tolerated the treatment well, with no adverse effects noted from the electrical pulses, even when several consecutive treatments were applied or when a high number of pulses was administered.
- No regrowth of the tumours was observed over an 18-month follow-up period.
Cite This Article
APA
Rols MP, Tamzali Y, Teissié J.
(2002).
Electrochemotherapy of horses. A preliminary clinical report.
Bioelectrochemistry, 55(1-2), 101-105.
https://doi.org/10.1016/s1567-5394(01)00156-6 Publication
Researcher Affiliations
- IPBS CNRS (UMR 5089), 205 route de Narbonne, 31077 Toulouse cedex, France.
MeSH Terms
- Animals
- Antineoplastic Agents / administration & dosage
- Antineoplastic Agents / therapeutic use
- Cisplatin / administration & dosage
- Cisplatin / therapeutic use
- Combined Modality Therapy
- Electric Stimulation Therapy
- Horse Diseases / drug therapy
- Horse Diseases / therapy
- Horses
- Injections, Intralesional
- Skin Neoplasms / drug therapy
- Skin Neoplasms / therapy
- Skin Neoplasms / veterinary
Citations
This article has been cited 7 times.- Tellado M, Mir LM, Maglietti F. Veterinary Guidelines for Electrochemotherapy of Superficial Tumors. Front Vet Sci 2022;9:868989.
- Mittal L, Aryal UK, Camarillo IG, Ferreira RM, Sundararajan R. Quantitative proteomic analysis of enhanced cellular effects of electrochemotherapy with Cisplatin in triple-negative breast cancer cells. Sci Rep 2019 Sep 26;9(1):13916.
- Guionet A, Moosavi Nejad S, Teissié J, Sakugawa T, Katsuki S, Akiyama H, Hosseini H. Spatio-temporal dynamics of calcium electrotransfer during cell membrane permeabilization. Drug Deliv Transl Res 2018 Oct;8(5):1152-1161.
- Venslauskas MS, Šatkauskas S. Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation. Eur Biophys J 2015 Jul;44(5):277-89.
- Chabot S, Pelofy S, Teissié J, Golzio M. Delivery of RNAi-Based Oligonucleotides by Electropermeabilization. Pharmaceuticals (Basel) 2013 Apr 10;6(4):510-21.
- Kotulska M. Natural fluctuations of an electropore show fractional Lévy stable motion. Biophys J 2007 Apr 1;92(7):2412-21.
- Mekid H, Tounekti O, Spatz A, Cemazar M, El Kebir FZ, Mir LM. In vivo evolution of tumour cells after the generation of double-strand DNA breaks. Br J Cancer 2003 Jun 2;88(11):1763-71.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
