FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Vet Immunol Immunopathol / Elevated extrahepatic expression and secretion of mammary-as...
Veterinary immunology and immunopathology2001; 83(3-4); 203-211; doi: 10.1016/s0165-2427(01)00380-4

Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum.

Abstract: Mammary-associated serum amyloid A 3 (M-SAA3) was secreted at highly elevated levels in bovine, equine and ovine colostrum and found at lower levels in milk 4 days postparturition. N-terminal sequencing of the mature M-SAA3 protein from all the three species revealed a conserved four amino acid motif (TFLK) within the first eight residues. This motif has not been reported to be present in any of the hepatically-produced acute phase SAA (A-SAA) isoforms. Cloning of the bovine M-Saa3 cDNA from mammary gland epithelial cells revealed an open reading frame that encoded a precursor protein of 131 amino acids which included an 18 amino acid signal peptide. The predicted 113 residue mature M-SAA3 protein had a theoretical molecular mass of 12,826Da that corresponded with the observed 12.8kDa molecular mass obtained for M-SAA3 in immunoblot analysis. The high abundance of this extrahepatically produced SAA3 isoform in the colostrum of healthy animals suggests that M-SAA3 may play an important functional role associated with newborn adaptation to extrauterine life and possibly mammary tissue remodeling.
Get Full Text
Publication Date: 2001-12-04 PubMed ID: 11730930DOI: 10.1016/s0165-2427(01)00380-4Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the high presence of Mammary-associated serum amyloid A 3 (M-SAA3) in the colostrum of bovines, equines, and ovines, and how it may play a significant role in newborn adaptation to post-womb life and in mammary tissue remodeling.

High Levels of M-SAA3 in Colostrum

  • The research started by noting the high level of M-SAA3 in the colostrum from cows, horses, and sheep.
  • It was also important to note that the presence of M-SAA3 was lower in the milk obtained 4 days after giving birth.

Conserved Motif in M-SAA3

  • The researchers discovered a conserved, or recurring, four amino acid motif (sequence) in M-SAA3’s mature protein from all three species examined.
  • This recurring sequence is not present in any of the isoforms of Serum Amyloid A (SAA) produced by the liver.

Bovine M-Saa3 Cloning

  • After cloning the bovine M-Saa3 gene from mammary gland epithelial cells, the team discovered that this gene encoded a precursor protein made up of 131 amino acids and included an 18 amino acid signal peptide.
  • The mature M-SAA3 protein is predicted to contain 113 residues and has a theoretical molecular mass of 12,826Da.
  • This estimate correlates with the observed 12.8kDa molecular mass found for M-SAA3 during the testing phase.

Potential Function of M-SAA3

  • Due to the high presence of this substance in colostrum from healthy animals, the study concluded that M-SAA3 evidently has a significant function associated with newborn adaptation to life outside the uterus.
  • The article suggests M-SAA3 may also play a part in the remodeling (cell regeneration and reshaping) of mammary tissue.

Cite This Article

APA
McDonald TL, Larson MA, Mack DR, Weber A. (2001). Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum. Vet Immunol Immunopathol, 83(3-4), 203-211. https://doi.org/10.1016/s0165-2427(01)00380-4

Publication

Veterinary immunology and immunopathology
ISSN: 0165-2427
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 83
Issue: 3-4
Pages: 203-211

Researcher Affiliations

McDonald, T L
  • Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USA. tmcdonal@unmc.edu
Larson, M A
    Mack, D R
      Weber, A

        MeSH Terms

        • Amino Acid Motifs / immunology
        • Amino Acid Sequence
        • Animals
        • Base Sequence
        • Cattle
        • Chromatography, Affinity / veterinary
        • Colostrum / chemistry
        • Colostrum / immunology
        • DNA / chemistry
        • Electrophoresis, Polyacrylamide Gel / veterinary
        • Enzyme-Linked Immunosorbent Assay / veterinary
        • Horses
        • Mammary Glands, Animal / metabolism
        • Milk / chemistry
        • Milk / immunology
        • Molecular Sequence Data
        • Molecular Weight
        • Protein Isoforms
        • RNA / chemistry
        • RNA / isolation & purification
        • Rabbits
        • Reverse Transcriptase Polymerase Chain Reaction / veterinary
        • Sequence Analysis, DNA
        • Sequence Homology, Amino Acid
        • Serum Amyloid A Protein / biosynthesis
        • Serum Amyloid A Protein / isolation & purification
        • Serum Amyloid A Protein / metabolism
        • Sheep

        Citations

        This article has been cited 34 times.
        1. Palmisano M, Javsicas L, McNaughten J, Gamsjäger L, Renaud DL, Gomez DE. Effect of plasma transfusion on serum amyloid A concentration in healthy neonatal foals and foals with failure of transfer of passive immunity.. J Vet Intern Med 2023 Mar;37(2):697-702.
          doi: 10.1111/jvim.16647pubmed: 36825688google scholar: lookup
        2. Fazio E, Bionda A, Liotta L, Amato A, Chiofalo V, Crepaldi P, Satué K, Lopreiato V. Changes of acute-phase proteins, glucose, and lipid metabolism during pregnancy in lactating dairy cows.. Arch Anim Breed 2022;65(3):329-339.
          doi: 10.5194/aab-65-329-2022pubmed: 36267480google scholar: lookup
        3. Addis MF, Maffioli EM, Penati M, Albertini M, Bronzo V, Piccinini R, Tangorra F, Tedeschi G, Cappelli G, Di Vuolo G, Vecchio D, De Carlo E, Ceciliani F. Peptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci.. Sci Rep 2022 May 19;12(1):8371.
          doi: 10.1038/s41598-022-12297-zpubmed: 35589845google scholar: lookup
        4. Giagu A, Penati M, Traini S, Dore S, Addis MF. Milk proteins as mastitis markers in dairy ruminants - a systematic review.. Vet Res Commun 2022 Jun;46(2):329-351.
          doi: 10.1007/s11259-022-09901-ypubmed: 35195874google scholar: lookup
        5. Kamiya S, Shimizu K, Okada A, Inoshima Y. Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid.. Animals (Basel) 2021 May 25;11(6).
          doi: 10.3390/ani11061548pubmed: 34070499google scholar: lookup
        6. Palombo V, Alharthi A, Batistel F, Parys C, Guyader J, Trevisi E, D'Andrea M, Loor JJ. Unique adaptations in neonatal hepatic transcriptome, nutrient signaling, and one-carbon metabolism in response to feeding ethyl cellulose rumen-protected methionine during late-gestation in Holstein cows.. BMC Genomics 2021 Apr 17;22(1):280.
          doi: 10.1186/s12864-021-07538-wpubmed: 33865335google scholar: lookup
        7. Otsuka M, Nishi Y, Tsukano K, Tsuchiya M, Lakritz J, Suzuki K. Sequential changes in hepatic mRNA abundance and serum concentration of serum amyloid A in cattle with acute inflammation caused by endotoxin.. J Vet Med Sci 2020 Jul 31;82(7):1006-1011.
          doi: 10.1292/jvms.19-0629pubmed: 32493888google scholar: lookup
        8. Murata E, Kozaki S, Murakami T, Shimizu K, Okada A, Ishiguro N, Inoshima Y. Differential expression of serum amyloid A1 and A3 in bovine epithelia.. J Vet Med Sci 2020 Jun 24;82(6):764-770.
          doi: 10.1292/jvms.19-0473pubmed: 32378645google scholar: lookup
        9. Katsafadou AI, Politis AP, Mavrogianni VS, Barbagianni MS, Vasileiou NGC, Fthenakis GC, Fragkou IA. Mammary Defences and Immunity against Mastitis in Sheep.. Animals (Basel) 2019 Sep 26;9(10).
          doi: 10.3390/ani9100726pubmed: 31561433google scholar: lookup
        10. Razavi SA, Pourjafar M, Hajimohammadi A, Valizadeh R, Naserian AA, Laven R, Mueller KR. Effects of dietary supplementation of bentonite and Saccharomyces cerevisiae cell wall on acute-phase protein and liver function in high-producing dairy cows during transition period.. Trop Anim Health Prod 2019 Jun;51(5):1225-1237.
          doi: 10.1007/s11250-019-01815-3pubmed: 30701451google scholar: lookup
        11. Sack GH Jr. Serum amyloid A - a review.. Mol Med 2018 Aug 30;24(1):46.
          doi: 10.1186/s10020-018-0047-0pubmed: 30165816google scholar: lookup
        12. Sack GH Jr, Zachara N, Rosenblum N, Talbot CC Jr, Kreimer S, Cole R, McDonald TL. Serum amyloid A1 (SAA1) protein in human colostrum.. FEBS Open Bio 2018 Mar;8(3):435-441.
          doi: 10.1002/2211-5463.12383pubmed: 29511620google scholar: lookup
        13. Bagga A, Randhawa SS, Sharma S, Bansal BK. Acute phase response in lame crossbred dairy cattle.. Vet World 2016 Nov;9(11):1204-1208.
          doi: 10.14202/vetworld.2016.1204-1208pubmed: 27956769google scholar: lookup
        14. Salvesen Ø, Reiten MR, Heegaard PM, Tranulis MA, Espenes A, Skovgaard K, Ersdal C. Activation of innate immune genes in caprine blood leukocytes after systemic endotoxin challenge.. BMC Vet Res 2016 Oct 28;12(1):241.
          doi: 10.1186/s12917-016-0870-xpubmed: 27793136google scholar: lookup
        15. De Buck M, Gouwy M, Wang JM, Van Snick J, Opdenakker G, Struyf S, Van Damme J. Structure and Expression of Different Serum Amyloid A (SAA) Variants and their Concentration-Dependent Functions During Host Insults.. Curr Med Chem 2016;23(17):1725-55.
          doi: 10.2174/0929867323666160418114600pubmed: 27087246google scholar: lookup
        16. Seo M, Lee HJ, Kim K, Caetano-Anolles K, Jeong JY, Park S, Oh YK, Cho S, Kim H. Characterizing Milk Production Related Genes in Holstein Using RNA-seq.. Asian-Australas J Anim Sci 2016 Mar;29(3):343-51.
          doi: 10.5713/ajas.15.0525pubmed: 26950864google scholar: lookup
        17. Hesselager MO, Codrea MC, Sun Z, Deutsch EW, Bennike TB, Stensballe A, Bundgaard L, Moritz RL, Bendixen E. The Pig PeptideAtlas: A resource for systems biology in animal production and biomedicine.. Proteomics 2016 Feb;16(4):634-44.
          doi: 10.1002/pmic.201500195pubmed: 26699206google scholar: lookup
        18. Thomas FC, Waterston M, Hastie P, Parkin T, Haining H, Eckersall PD. The major acute phase proteins of bovine milk in a commercial dairy herd.. BMC Vet Res 2015 Aug 15;11:207.
          doi: 10.1186/s12917-015-0533-3pubmed: 26276568google scholar: lookup
        19. Paltrinieri S, Sironi G, Giori L, Faverzani S, Longeri M. Changes in serum and urine SAA concentrations and qualitative and quantitative proteinuria in Abyssinian cats with familial amyloidosis: a five-year longitudinal study (2009-2014).. J Vet Intern Med 2015 Mar-Apr;29(2):505-12.
          doi: 10.1111/jvim.12561pubmed: 25776129google scholar: lookup
        20. Gaffney PM, Barr B, Rowe JD, Bett C, Drygiannakis I, Giannitti F, Trejo M, Ghassemian M, Martin P, Masliah E, Sigurdson CJ. Protein profiling of isolated uterine AA amyloidosis causing fetal death in goats.. FASEB J 2015 Mar;29(3):911-9.
          doi: 10.1096/fj.14-256081pubmed: 25422367google scholar: lookup
        21. Hernández-Castellano LE, Almeida AM, Ventosa M, Coelho AV, Castro N, Argüello A. The effect of colostrum intake on blood plasma proteome profile in newborn lambs: low abundance proteins.. BMC Vet Res 2014 Apr 5;10:85.
          doi: 10.1186/1746-6148-10-85pubmed: 24708841google scholar: lookup
        22. Cui X, Hou Y, Yang S, Xie Y, Zhang S, Zhang Y, Zhang Q, Lu X, Liu GE, Sun D. Transcriptional profiling of mammary gland in Holstein cows with extremely different milk protein and fat percentage using RNA sequencing.. BMC Genomics 2014 Mar 24;15:226.
          doi: 10.1186/1471-2164-15-226pubmed: 24655368google scholar: lookup
        23. Bundgaard L, Jacobsen S, Sørensen MA, Sun Z, Deutsch EW, Moritz RL, Bendixen E. The Equine PeptideAtlas: a resource for developing proteomics-based veterinary research.. Proteomics 2014 Mar;14(6):763-73.
          doi: 10.1002/pmic.201300398pubmed: 24436130google scholar: lookup
        24. Olsen HG, Skovgaard K, Nielsen OL, Leifsson PS, Jensen HE, Iburg T, Heegaard PM. Organization and biology of the porcine serum amyloid A (SAA) gene cluster: isoform specific responses to bacterial infection.. PLoS One 2013;8(10):e76695.
          doi: 10.1371/journal.pone.0076695pubmed: 24146912google scholar: lookup
        25. Tóthová C, Nagy O, Seidel H, Kováč G. The effect of storage temperature and time on the concentrations of bovine serum amyloid a and its mammary associated isoform.. Vet Med Int 2012;2012:861458.
          doi: 10.1155/2012/861458pubmed: 22577611google scholar: lookup
        26. Jain S, Gautam V, Naseem S. Acute-phase proteins: As diagnostic tool.. J Pharm Bioallied Sci 2011 Jan;3(1):118-27.
          doi: 10.4103/0975-7406.76489pubmed: 21430962google scholar: lookup
        27. Reigstad CS, Bäckhed F. Microbial regulation of SAA3 expression in mouse colon and adipose tissue.. Gut Microbes 2010 Jan;1(1):55-57.
          doi: 10.4161/gmic.1.1.10514pubmed: 21327118google scholar: lookup
        28. Skovgaard K, Mortensen S, Boye M, Poulsen KT, Campbell FM, Eckersall PD, Heegaard PM. Rapid and widely disseminated acute phase protein response after experimental bacterial infection of pigs.. Vet Res 2009 May-Jun;40(3):23.
          doi: 10.1051/vetres/2009006pubmed: 19236838google scholar: lookup
        29. de Jesus Rodriguez B, Chevaleyre C, Henry G, Mollé D, Virlogeux-Payant I, Berri M, Boulay F, Léonil J, Meurens F, Salmon H. Identification in milk of a serum amyloid A peptide chemoattractant for B lymphoblasts.. BMC Immunol 2009 Jan 23;10:4.
          doi: 10.1186/1471-2172-10-4pubmed: 19166592google scholar: lookup
        30. Wheeler TT, Hodgkinson AJ, Prosser CG, Davis SR. Immune components of colostrum and milk--a historical perspective.. J Mammary Gland Biol Neoplasia 2007 Dec;12(4):237-47.
          doi: 10.1007/s10911-007-9051-7pubmed: 17992474google scholar: lookup
        31. Shahriar F, Gordon JR, Simko E. Identification of lipopolysaccharide-binding proteins in porcine milk.. Can J Vet Res 2006 Oct;70(4):243-50.
          pubmed: 17042375
        32. Gruys E, Toussaint MJ, Niewold TA, Koopmans SJ. Acute phase reaction and acute phase proteins.. J Zhejiang Univ Sci B 2005 Nov;6(11):1045-56.
          doi: 10.1631/jzus.2005.B1045pubmed: 16252337google scholar: lookup
        33. Gruys E, Toussaint MJ, Upragarin N, Van EA, Adewuyi AA, Candiani D, Nguyen TK, Sabeckiene J. Acute phase reactants, challenge in the near future of animal production and veterinary medicine.. J Zhejiang Univ Sci B 2005 Oct;6(10):941-7.
          doi: 10.1631/jzus.2005.B0941pubmed: 16187407google scholar: lookup
        34. Gruys E. Protein folding pathology in domestic animals.. J Zhejiang Univ Sci 2004 Oct;5(10):1226-38.
          doi: 10.1631/jzus.2004.1226pubmed: 15362194google scholar: lookup
        Subscribe to our newsletter
        Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.