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Home / Equine Research Bank / BMC Vet Res / Elevated urinary excretion of free pyridinoline in Friesian ...
BMC veterinary research2018; 14(1); 139; doi: 10.1186/s12917-018-1454-8

Elevated urinary excretion of free pyridinoline in Friesian horses suggests a breed-specific increase in collagen degradation.

Abstract: Friesian horses are known for their high inbreeding rate resulting in several genetic diseases such as hydrocephaly and dwarfism. This last decade, several studies focused on two other presumed hereditary traits in Friesian horses: megaoesophagus and aortic rupture. The pathogenesis of these diseases remains obscure but an important role of collagen has been hypothesized. The purpose of this study was to examine possible breed-related differences in collagen catabolism. Urinary specimens from Friesian (n = 17, median age 10 years old) and Warmblood horses (n = 17, median age 10 years old) were assessed for mature collagen cross-links, i.e. pyridinoline (PYD) (=hydroxylysylpyridinoline/HP) and deoxypyridinoline (DPD) (lysylpyridinoline /LP). Solid-phase extraction was performed, followed by reversed-phase ion-paired liquid chromatography prior to tandem mass spectrometry (MS/MS) detection. Results: Mean urinary concentrations of free PYD, expressed as fPYD/creatinine ratio, were significantly higher in Friesian horses compared to Warmblood horses (28.5 ± 5.2 versus 22.2 ± 9.6 nmol/mmol, p = 0.02) while mean fDPD/creatinine ratios were similar in both horse breeds (3.0 ± 0.7 versus 4.6 ± 3.7 nmol/mmol, p = 0.09). Conclusions: Since DPD is considered a specific bone degradation marker and PYD is more widely distributed in connective tissues, the significant elevation in the mean PYD/DPD ratio in Friesian versus Warmblood horses (9.6 ± 1.6 versus 5.7 ± 1.8, p < 0.0001) suggests a soft tissue origin for the increased fPYD levels. Considering that a previous study found no differences in total collagen content between Friesian and Warmblood horses for tendon and aortic tissue, this indicates a higher rate of collagen degradation. The latter might, at least in part, explain the predisposition of Friesians to connective tissue disorders.
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Publication Date: 2018-04-25 PubMed ID: 29699546PubMed Central: PMC5921786DOI: 10.1186/s12917-018-1454-8Google Scholar: Lookup
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Summary

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This research highlights a breed-specific increase in collagen degradation in Friesian horses, suggested by elevated urinary excretion of free pyridinoline. This connective tissue disorder might explain why Friesian horses are known to be more prone to certain genetic diseases.

Research Overview

  • The main aim of this study was to inspect possible breed-related differences in the rate of collagen breakdown among horses.
  • Specimens of urine from Friesian and Warmblood horses, were examined for mature collagen cross-links, such as pyridinoline (PYD) and deoxypyridinoline (DPD).
  • Apart from traditional techniques, the research team also used methods like reversed-phase ion-paired liquid chromatography and tandem mass spectrometry for detection.

Findings

  • The scientists found that the concentrations of free PYD were significantly higher in Friesian horses when compared to Warmblood horses.
  • However, the mean fDPD/creatinine ratios were similar in both horse breeds.
  • Since DPD is a specific marker of bone degradation and PYD is found more in connective tissues, this significant surge in the PYD/DPD ratio suggests a soft tissue origin for the increased PYD levels in Friesian horses.

Conclusions

  • The unique increase in PYD levels and the PYD/DPD ratio indicates a higher rate of collagen degradation in Friesian horses compared to other breeds.
  • This discovery may help partially explain the predisposition of Friesian horses to certain genetic diseases and connective tissue disorders, since the quantity of collagen in the body directly affects the condition of connective tissues, which in turn can predispose to certain diseases.

Cite This Article

APA
Saey V, Tang J, Ducatelle R, Croubels S, De Baere S, Schauvliege S, van Loon G, Chiers K. (2018). Elevated urinary excretion of free pyridinoline in Friesian horses suggests a breed-specific increase in collagen degradation. BMC Vet Res, 14(1), 139. https://doi.org/10.1186/s12917-018-1454-8

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 14
Issue: 1
Pages: 139

Researcher Affiliations

Saey, Veronique
  • Laboratory of Veterinary Pathology, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. veronique.saey@ugent.be.
Tang, Jonathan
  • Bioanalytical Facility, Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, NR4 7UQ, UK.
Ducatelle, Richard
  • Laboratory of Veterinary Pathology, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Croubels, Siska
  • Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
De Baere, Siegrid
  • Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Schauvliege, Stijn
  • Deparment of Surgery and anaesthesiology of domestic animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
van Loon, Gunther
  • Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Chiers, Koen
  • Laboratory of Veterinary Pathology, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

MeSH Terms

  • Amino Acids / urine
  • Animals
  • Collagen / metabolism
  • Collagen Diseases / genetics
  • Collagen Diseases / urine
  • Collagen Diseases / veterinary
  • Female
  • Horse Diseases / genetics
  • Horse Diseases / metabolism
  • Horse Diseases / urine
  • Horses
  • Inbreeding
  • Male
  • Species Specificity

Conflict of Interest Statement

ETHICS APPROVAL: An approved animal use protocol was waived by the chairperson of the ethical committee of the Faculty of Veterinary Medicine (Ghent University) as urine in the awake horses was collected by the owner/keeper not invasively during spontaneous voiding. The anesthetized horses were sampled by urinary catheterization as standard procedure during operation. A written consent was obtained from the owner/keeper of each animal. All of the patients that were omitted to the University hospital, were treated following the institutional guidelines. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Citations

This article has been cited 3 times.
  1. de Meeûs d'Argenteuil C, Boshuizen B, Oosterlinck M, van de Winkel D, De Spiegelaere W, de Bruijn CM, Goethals K, Vanderperren K, Delesalle CJG. Flexibility of equine bioenergetics and muscle plasticity in response to different types of training: An integrative approach, questioning existing paradigms. PLoS One 2021;16(4):e0249922.
    doi: 10.1371/journal.pone.0249922pubmed: 33848308google scholar: lookup
  2. Casola C, Pot SA, Lavaud A, Voelter K. Corneal cross-linking as a treatment for corneal dystrophy with secondary bacterial infection in a Friesian horse. Clin Case Rep 2020 Apr;8(4):709-715.
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  3. Vera L, De Clercq D, Van Steenkiste G, Decloedt A, Chiers K, van Loon G. Differences in ultrasound-derived arterial wall stiffness parameters and noninvasive blood pressure between Friesian horses and Warmblood horses. J Vet Intern Med 2020 Mar;34(2):893-901.
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