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Home / Equine Research Bank / Toxins (Basel) / Emergence of Nasal Carriage of ST80 and ST152 PVL+ Staphyloc...
Toxins2017; 9(10); doi: 10.3390/toxins9100303

Emergence of Nasal Carriage of ST80 and ST152 PVL+ Staphylococcus aureus Isolates from Livestock in Algeria.

Abstract: The spread of toxinogenic Staphylococcus aureus is a public health problem in Africa. The objectives of the study were to investigate the rate of S. aureus nasal carriage and molecular characteristics of these strains in livestock and humans in three Algerian provinces. Nasal samples were collected from camels, horses, cattle, sheep and monkeys, as well as humans in contact with them. S. aureus isolates were genotyped using DNA microarray. The rate of S. aureus nasal carriage varied between species: camels (53%), humans and monkeys (50%), sheep (44.2%), horses (15.2%) and cattle (15%). Nine methicillin-resistant S. aureus (MRSA) isolates (7.6%) were identified, isolated from camels and sheep. The S. aureus isolates belonged to 15 different clonal complexes. Among them, PVL+ (Panton-Valentine Leukocidin) isolates belonging to ST80-MRSA-IV and ST152-MSSA were identified in camels (n = 3, 13%) and sheep (n = 4, 21.1%). A high prevalence of toxinogenic animal strains was noted containing TSST-1- (22.2%), EDINB- (29.6%) and EtD- (11.1%) encoding genes. This study showed the dispersal of the highly human pathogenic clones ST152-MSSA and ST-80-MRSA in animals. It suggests the ability of some clones to cross the species barrier and jump between humans and several animal species.
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Publication Date: 2017-09-25 PubMed ID: 28946704PubMed Central: PMC5666350DOI: 10.3390/toxins9100303Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers investigated the prevalence and genetic characteristics of a specific type of toxin-producing Staphylococcus aureus bacteria in livestock and humans in Algeria. They discovered that particularly harmful strains of these bacteria can spread between humans and various animal species.

Research Methodology

  • The study was conducted in three Algerian provinces, with the main focus on determining the rate of nasal carriage (presence) of Staphylococcus aureus (a type of bacteria) among different species.
  • Nasal samples were obtained from camels, horses, cattle, sheep, monkeys, as well as humans in close contact with these animals.
  • These samples were subsequently genotyped using DNA microarray, a lab-on-a-chip tool used to detect and measure the expression of thousands of genes at once.

Major Findings

  • The rate of nasal carriage of the bacteria varied across species: camels (53%), humans, monkeys (50%), sheep (44.2%), horses (15.2%), and cattle (15%).
  • Out of the samples, nine methicillin-resistant Staphylococcus aureus (MRSA) isolates (7.6%) were identified, particularly isolated from camels and sheep. This type of bacteria is infamous for its resistance to antibiotics, making infections hard to treat.
  • They found that bacteria belonging to 15 different clonal complexes—groups of bacteria descended from a common ancestor. Particularly, Panton-Valentine Leukocidin-producing (PVL+) isolates tied to ST80-MRSA-IV and ST152-MSSA clonal complexities were found in camels and sheep. PVL+ refers to the bacteria’s ability to produce a toxin that destroys white blood cells.

Noteworthy Implications

  • This study discovered a high prevalence of toxinogenic or toxin-producing animal strains. These included strains that contained certain types of harmful genes, such as TSST-1-, EDINB-, and EtD-.
  • A significant implication of this research is the evidence of the spread of the highly pathogenic (disease-causing) clones ST152-MSSA and ST-80-MRSA among animals.
  • The study findings suggest the likelihood of some bacterial clones to break the species barrier and jump from humans to different animal species and vice versa. This suggests a potential risk of zoonotic infections, where diseases can be transmitted from animals to humans.

Cite This Article

APA
Agabou A, Ouchenane Z, Ngba Essebe C, Khemissi S, Chehboub MTE, Chehboub IB, Sotto A, Dunyach-Remy C, Lavigne JP. (2017). Emergence of Nasal Carriage of ST80 and ST152 PVL+ Staphylococcus aureus Isolates from Livestock in Algeria. Toxins (Basel), 9(10). https://doi.org/10.3390/toxins9100303

Publication

Toxins
ISSN: 2072-6651
NlmUniqueID: 101530765
Country: Switzerland
Language: English
Volume: 9
Issue: 10

Researcher Affiliations

Agabou, Amir
  • Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier, UFR de Médecine, 30908 Nîmes, France. amirveto@gmail.com.
  • Laboratoire PADESCA, Institut des Sciences Vétérinaires, Université des Frères Mentouri-Constantine 1, Constantine 25017, Algérie. amirveto@gmail.com.
Ouchenane, Zouleikha
  • Laboratoire de microbiologie, Hôpital Militaire Régional Universitaire de Constantine, Constantine 25001, Algérie. z_ouche@yahoo.fr.
Ngba Essebe, Christelle
  • Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier, UFR de Médecine, 30908 Nîmes, France. ngbachristelle@yahoo.fr.
Khemissi, Salim
  • Laboratoire de microbiologie, Hôpital Militaire Régional Universitaire de Constantine, Constantine 25001, Algérie. salimkhemissi@gmail.com.
Chehboub, Mohamed Tedj Eddine
  • Laboratoire PADESCA, Institut des Sciences Vétérinaires, Université des Frères Mentouri-Constantine 1, Constantine 25017, Algérie. tedj.chehboub@gmail.com.
Chehboub, Ilyes Bey
  • Laboratoire PADESCA, Institut des Sciences Vétérinaires, Université des Frères Mentouri-Constantine 1, Constantine 25017, Algérie. ilyesbeycheboub@gmail.com.
Sotto, Albert
  • Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier, UFR de Médecine, 30908 Nîmes, France. albert.sotto@chu-nimes.fr.
  • Service des Maladies Infectieuses et Tropicales, CHU Nîmes, 30029 Nîmes, France. albert.sotto@chu-nimes.fr.
Dunyach-Remy, Catherine
  • Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier, UFR de Médecine, 30908 Nîmes, France. catherine.remy@chu-nimes.fr.
  • Service de Microbiologie, CHU Nîmes, 30029 Nîmes, France. catherine.remy@chu-nimes.fr.
Lavigne, Jean-Philippe
  • Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier, UFR de Médecine, 30908 Nîmes, France. jean.philippe.lavigne@chu-nimes.fr.
  • Service de Microbiologie, CHU Nîmes, 30029 Nîmes, France. jean.philippe.lavigne@chu-nimes.fr.

MeSH Terms

  • Algeria
  • Animals
  • Camelus
  • Carrier State / microbiology
  • Cattle
  • Haplorhini
  • Horses
  • Humans
  • Livestock
  • Molecular Epidemiology
  • Nose / microbiology
  • Sheep
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / isolation & purification

Conflict of Interest Statement

The authors declare no conflict of interest. The funding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

References

This article includes 35 references
  1. McCaig LF, McDonald LC, Mandal S, Jernigan DB. Staphylococcus aureus-associated skin and soft tissue infections in ambulatory care.. Emerg. Infect. Dis. 2006;12:1715–1723.
    doi: 10.3201/eid1211.060190pmc: PMC3372331pubmed: 17283622google scholar: lookup
  2. Wertheim HF, Melles DC, Vos MC, Van Leeuwen W, Van Belkum A, Verbrugh HA, Nouwen JL. The role of nasal carriage in Staphylococcus aureus infections.. Lancet Infect. Dis. 2005;5:751–762.
    doi: 10.1016/S1473-3099(05)70295-4pubmed: 16310147google scholar: lookup
  3. Iverson SA, Brazil AM, Ferguson JM, Nelson K, Lautenbach E, Rankin SC, Morris DO, Davis MF. Anatomical patterns of colonization of pets with staphylococcal species in homes of people with methicillin-resistant Staphylococcus aureus (MRSA) skin or soft tissue infection (SSTI). Vet. Microbiol. 2015;176:202–208.
    doi: 10.1016/j.vetmic.2015.01.003pubmed: 25623014google scholar: lookup
  4. Shen H, Akoda E, Zhang K. Methicillin-Resistant Staphylococcus aureus carriage among students at a historically black university: A Case Study.. Int. J. Microbiol. 2013;2013:979734.
    doi: 10.1155/2013/979734pmc: PMC3563209pubmed: 23401691google scholar: lookup
  5. Woolhouse M, Ward M, Van Bunnik B, Farrar J. Antimicrobial resistance in humans, livestock and the wider environment.. Philos. Trans. R. Soc. Lond. B Biol. Sci. 2015;370:20140083.
    doi: 10.1098/rstb.2014.0083pmc: PMC4424433pubmed: 25918441google scholar: lookup
  6. Graveland H, Wagenaar JA, Bergs K, Heesterbeek H, Heederik D. Persistence of livestock associated MRSA CC398 in humans is dependent on intensity of animal contact.. PLoS ONE 2011;6:e16830.
    doi: 10.1371/journal.pone.0016830pmc: PMC3036727pubmed: 21347386google scholar: lookup
  7. Abdulgader SM, Shittu AO, Nicol MP, Kaba M. Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Africa: A systematic review.. Front. Microbiol. 2015;6:348.
    doi: 10.3389/fmicb.2015.00348pmc: PMC4415431pubmed: 25983721google scholar: lookup
  8. Gharsa H, Slama KB, Lozano C, Gómez-Sanz E, Klibi N, Sallem RB, Gómez P, Zarazaga M, Boudabous A, Torres C. Prevalence, antibiotic resistance, virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia.. Vet. Microbiol. 2012;156:367–373.
    doi: 10.1016/j.vetmic.2011.11.009pubmed: 22176760google scholar: lookup
  9. Raji MA, Garaween G, Ehricht R, Monecke S, Shibl AM, Senok A. Genetic Characterization of Staphylococcus aureus isolated from retail meat in Riyadh, Saudi Arabia.. Front. Microbiol. 2016;7:911.
    doi: 10.3389/fmicb.2016.00911pmc: PMC4899468pubmed: 27375611google scholar: lookup
  10. Stegger M, Wirth T, Andersen PS, Skov RL, De Grassi A, Simões PM, Tristan A, Petersen A, Aziz M, Kiil K. Origin and evolution of European community-acquired methicillin-resistant Staphylococcus aureus.. MBio 2014;5:e01044-14.
    doi: 10.1128/mBio.01044-14pmc: PMC4173770pubmed: 25161186google scholar: lookup
  11. Djahmi N, Messad N, Nedjai S, Moussaoui A, Mazouz D, Richard JL, Sotto A, Lavigne JP. Molecular epidemiology of Staphylococcus aureus strains isolated from inpatients with infected diabetic foot ulcers in an Algerian University Hospital.. Clin. Microbiol. Infect. 2013;19:E398–E404.
    doi: 10.1111/1469-0691.12199pubmed: 23521557google scholar: lookup
  12. Schaumburg F, Köck R, Friedrich AW, Soulanoudjingar S, Ngoa UA, von Eiff C, Issifou S, Kremsner PG, Hermann M, Peters G. Population structure of Staphylococcus aureus from remote African Babongo Pygmies.. PLoS Negl. Trop. Dis. 2011;5:e1150.
    doi: 10.1371/journal.pntd.0001150pmc: PMC3091839pubmed: 21572985google scholar: lookup
  13. Lozano C, Gharsa H, Ben Slama K, Zarazaga M, Torres C. Staphylococcus aureus in animals and food: Methicillin resistance, prevalence and population structure. A review in the African continent.. Microorganisms 2016;4:E12.
    doi: 10.3390/microorganisms4010012pmc: PMC5029517pubmed: 27681906google scholar: lookup
  14. Al Laham N, Mediavilla JR, Chen L, Abdelateef N, Elamreen FA, Ginocchio CC, Pierard D, Becker K, Kreiswirth BN. MRSA clonal complex 22 strains harboring toxic shock syndrome toxin (TSST-1) are endemic in the primary hospital in Gaza, Palestine.. PLoS ONE 2015;10:e0120008.
    doi: 10.1371/journal.pone.0120008pmc: PMC4364023pubmed: 25781188google scholar: lookup
  15. Biber A, Abuelaish I, Rahav G, Raz M, Cohen L, Valinsky L, Taran D, Goral A, Elhamdany A, Regev-Yochay G. A typical hospital-acquired methicillin-resistant Staphylococcus aureus clone is widespread in the community in the Gaza strip.. PLoS ONE 2012;7:e42864.
    doi: 10.1371/journal.pone.0042864pmc: PMC3420888pubmed: 22916171google scholar: lookup
  16. Rasigade JP, Laurent F, Lina G, Meugnier H, Bes M, Vandenesch F, Etienne J, Tristan A. Global distribution and evolution of Panton-Valentine leukocidin-positive methicillin-susceptible Staphylococcus aureus, 1981–2007.. J. Infect. Dis. 2010;201:1589–1597.
    doi: 10.1086/652008pubmed: 20367458google scholar: lookup
  17. Schaumburg F, Pauly M, Anoh E, Mossoun A, Wiersma L, Schubert G, Flammen A, Alabi AS, Muyembe-Tamfum JJ, Grobusch MP. Staphylococcus aureus complex from animals and humans in three remote African regions.. Clin. Microbiol. Infect. 2015;21:e1–e8.
    doi: 10.1016/j.cmi.2014.12.001pubmed: 25596779google scholar: lookup
  18. Boss R, Cosandey A, Luini M, Artursson K, Bardiau M, Breitenwieser F, Hehenberger E, Lam T, Mansfeld M, Michel A. Bovine Staphylococcus aureus: Subtyping, evolution, and zoonotic transfer.. J. Dairy Sci. 2016;99:515–528.
    doi: 10.3168/jds.2015-9589pubmed: 26601578google scholar: lookup
  19. Budd KE, McCoy F, Monecke S, Cormican P, Mitchell J, Keane OM. Extensive genomic diversity among bovine-adapted Staphylococcus aureus: Evidence for a genomic rearrangement within CC97.. PLoS ONE 2015;10:e0134592.
    doi: 10.1371/journal.pone.0134592pmc: PMC4552844pubmed: 26317849google scholar: lookup
  20. Burton S, Reid-Smith R, McClure JT, Weese JS. Staphylococcus aureus colonization in healthy horses in Atlantic Canada.. Can. Vet. J. 2008;49:797–799.
    pmc: PMC2465786pubmed: 18978975
  21. Mai-siyama IB, Okon KO, Adamu NB, Askira UM, Isyaka TM, Adamu SG. Methicillin-resistant Staphylococcus aureus (MRSA) colonization rate among ruminant animals slaughtered for human consumption and contact persons in Maiduguri, Nigeria.. Afr. J. Microbiol. Res. 2014;8:2643–2649.
    doi: 10.5897/AJMR2014.6855google scholar: lookup
  22. Schaumburg F, Mugisha L, Peck B, Becker K, Gillespie TR, Peters G, Leendertz FH. Drug-resistant human Staphylococcus aureus in sanctuary apes pose a threat to endangered wild ape populations.. Am. J. Primatol. 2012;74:1071–1075.
    doi: 10.1002/ajp.22067pubmed: 22907634google scholar: lookup
  23. Jaradat Z, Al Aboudi A, Shatnawi M, Ababneh Q. Staphylococcus aureus isolates from camels differ in coagulase production, genotype and methicillin resistance gene profiles.. J. Microbiol. Biotechnol. Food Sci. 2013;2:2455–2461.
  24. Al-Thani RF, Al-Ali F. Incidences and antimicrobial susceptibility profile of Staphylococcus species isolated from animals in different Qatari farms.. Afr. J. Microbiol. 2012;6:7454–7458.
    doi: 10.5897/AJMR12.1270google scholar: lookup
  25. Alzohairy MA. Colonization and antibiotic susceptibility pattern of methicillin resistance Staphylococcus aureus (MRSA) among farm animals in Saudi Arabia.. Afr. J. Bacteriol. Res. 2011;3:63–68.
  26. Vautor E, Abadie G, Guibert JM, Chevalier N, Pepin M. Nasal carriage of Staphylococcus aureus in dairy sheep.. Vet. Microbiol. 2005;106:235–239.
    doi: 10.1016/j.vetmic.2004.11.019pubmed: 15778029google scholar: lookup
  27. Sung JM, Lloyd DH, Lindsay JA. Staphylococcus aureus host specificity: Comparative genomics of human versus animal isolates by multi-strain microarray.. Microbiology 2008;154:1949–1959.
    doi: 10.1099/mic.0.2007/015289-0pubmed: 18599823google scholar: lookup
  28. Herron-Olson L, Fitzgerald JR, Musser JM, Kapur V. Molecular correlates of host specialization in Staphylococcus aureus.. PLoS ONE 2007;2:e1120.
    doi: 10.1371/journal.pone.0001120pmc: PMC2040198pubmed: 17971880google scholar: lookup
  29. Van Duijkeren E, Wolfhagen MJ, Heck ME, Wannet WJ. Transmission of a Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain between humans and a dog.. J. Clin. Microbiol. 2005;43:6209–6211.
    doi: 10.1128/JCM.43.12.6209-6211.2005pmc: PMC1317200pubmed: 16333133google scholar: lookup
  30. Fall C, Seck A, Richard V, Ndour M, Sembene M, Laurent F, Breurec S. Epidemiology of Staphylococcus aureus in pigs and farmers in the largest farm in Dakar, Senegal.. Foodborne Pathog. Dis. 2012;9:962–965.
    doi: 10.1089/fpd.2012.1197pubmed: 22953738google scholar: lookup
  31. Rainard P, Corrales JC, Barrio MB, Cochard T, Poutrel B. Leucotoxic activities of Staphylococcus aureus strains isolated from cows, ewes, and goats with mastitis: Importance of LukM/LukF-PV leukotoxin.. Clin. Diagn. Lab. Immunol. 2003;10:272–277.
    doi: 10.1128/CDLI.10.2.272-277.2003pmc: PMC150537pubmed: 12626454google scholar: lookup
  32. Kuroda M, Ohta T, Uchiyama I, Baba T, Yuzawa H, Kobayashi I, Cui L, Oguchi A, Aoki KI, Nagai Y. Whole genome sequencing of meticillin-resistant Staphylococcus aureus.. Lancet 2001;357:1225–1240.
    doi: 10.1016/S0140-6736(00)04403-2pubmed: 11418146google scholar: lookup
  33. Haveri M, Roslöf A, Rantala L, Pyörälä S. Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics.. J. Appl. Microbiol. 2007;103:993–1000.
    doi: 10.1111/j.1365-2672.2007.03356.xpubmed: 17897203google scholar: lookup
  34. EUCAST-CASFM. [(accessed on 12 August 2017)]; Available online: http://www.sfm-microbiologie.org.
  35. Monecke S, Coombs G, Shore AC, Coleman DC, Akpaka P, Borg M, Chow H, Ip M, Jatzwauk L, Jonas D. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus.. PLoS ONE 2011;6:e17936.
    doi: 10.1371/journal.pone.0017936pmc: PMC3071808pubmed: 21494333google scholar: lookup

Citations

This article has been cited 28 times.
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