Endocrine-paracrine cells of the male urogenital apparatus: a comparative histochemical and immunohistochemical study in some domestic ungulates.
Abstract: Specimens of testis, excurrent duct including the male accessory glands and urethra, were studied in boars, bulls, horses and donkeys, in order to localize endocrine/paracrine cells. Silver impregnation methods were used to test the argentaffinity and/or argyrophilia of cells. Immunoreactivities to chromogranin A, 5-hydroxytryptamine, somatostatin, [met]- and [leu]- enkephalins, gastrin-releasing peptide, calcitonin gene-related peptide, neuropeptide Y, substance P, vasoactive intestinal peptide, beta-endorphin antisera were tested by a streptavidin-biotin method. In the testis, epididymis, ductus deferens and vesicular gland no endocrine cells were found in any of the animals studied. Chromogranin-A, serotonin, somatostatin and enkephalins were present in endocrine/paracrine cells in the surface or glandular epithelia, whereas all other antisera gave negative results. In the prostatic complex and the urethral epithelium, the most consistent number of endocrine cells was serotonin-immunoreactive. Few cells were also argentaffin and a very limited number of them showed argyrophily and chromogranin-A immunoreactivity. Somatostatin-and enkephalin-immunoreactive cells were rare in the bull and boar, absent in stallions. This comparative study carried out on different species of domestic ungulates has shown deeply different immunophenotypes, even comparing species that are in a very close zoological relationship with one another, such as the horse and the donkey.
Publication Date: 2004-07-09 PubMed ID: 15239814DOI: 10.1111/j.1439-0264.2004.00541.xGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article focused on studying endocrine and paracrine cells within the male urogenital system of various large domestic animals, specifically boars, bulls, horses, and donkeys.
Research Methods
- This study utilized specimens of the testis, the excurrent duct, male accessory glands, and urinary tract from different large domestic animals: boars, bulls, horses, and donkeys.
- Two main histochemical methods were applied: Silver impregnation, which is used to visualize certain types of cells, and immunohistochemistry, a method that uses antibodies to detect specific proteins and other substances in cells. These approaches helped identify the presence and location of endocrine and paracrine cells.
- Immunoreactivity tests for chromogranin A, 5-hydroxytryptamine, somatostatin, [met]-, [leu]- enkephalins, gastrin-releasing peptide, calcitonin gene-related peptide, neuropeptide Y, substance P, vasoactive intestinal peptide, beta-endorphin antisera were conducted using the streptavidin-biotin technique.
Research Findings
- Endocrine cells were not found within the testis, epididymis, ductus deferens, or vesicular gland on any of the animals studied.
- Cells showing chromogranin-A, serotonin, somatostatin, and enkephalins were detected on the surface or within glandular epithelia. Other tested antisera gave negative results, meaning that their presence could not be confirmed.
- In the prostatic complex and the urethral epithelium, serotonin-immunoreactive endocrine cells were most prevalent. Few cells demonstrated argentaffinity, and a limited number showed argyrophily and chromogranin-A immunoreactivity.
- Cells showing reactivity to somatostatin and enkephalins were rare in bulls and boars, and completely absent in horses.
Conclusion
- This study showed profound differences in the immunophenotype of endocrine and paracrine cells between different species of domestic ungulates, even those closely related like horses and donkeys.
- This may be significant in understanding the variance in reproductive physiology across different species.
Cite This Article
APA
Arrighi S, Cremonesi F, Bosi G, Domeneghini C.
(2004).
Endocrine-paracrine cells of the male urogenital apparatus: a comparative histochemical and immunohistochemical study in some domestic ungulates.
Anat Histol Embryol, 33(4), 225-232.
https://doi.org/10.1111/j.1439-0264.2004.00541.x Publication
Researcher Affiliations
- Department of Veterinary Sciences and Technologies for Food Safety, Faculty of Veterinary Medicine, Milan, Italy. silvana.arrighi@unimi.it
MeSH Terms
- Animals
- Cattle / anatomy & histology
- Endocrine Glands / cytology
- Endocrine Glands / metabolism
- Equidae / anatomy & histology
- Genitalia, Male / cytology
- Genitalia, Male / metabolism
- Histocytochemistry / veterinary
- Horses / anatomy & histology
- Immunohistochemistry / veterinary
- Male
- Silver Staining / veterinary
- Species Specificity
- Sus scrofa / anatomy & histology
- Urethra / cytology
- Urethra / metabolism
Citations
This article has been cited 3 times.- Başar MM, Han Ü, Çakan M, Alpcan S, Başar H. Orexin expression in different prostate histopathologic examinations: Can it be a marker for prostate cancer? A preliminary result. Turk J Urol 2013 Jun;39(2):78-83.
- Jiménez-Trejo F, Tapia-Rodríguez M, Cerbón M, Kuhn DM, Manjarrez-Gutiérrez G, Mendoza-Rodríguez CA, Picazo O. Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility. Reproduction 2012 Dec;144(6):677-85.
- Arrighi S, Bosi G, Cremonesi F, Domeneghini C. Immunohistochemical study of the pre- and postnatal innervation of the dog lower urinary tract: morphological aspects at the basis of the consolidation of the micturition reflex. Vet Res Commun 2008 Apr;32(4):291-304.
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