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Frontiers in microbiology2022; 13; 884574; doi: 10.3389/fmicb.2022.884574

Endometrial and vaginal microbiome in donkeys with and without clinical endometritis.

Abstract: Endometrial and vaginal microbiomes are critical in the study of endometritis, which is an important cause of infertility in donkeys. Our objective was to investigate the difference of the endometrial and vaginal microbiomes between healthy donkey jennies (group C) and jennies with endometritis (group E). Endometrial and vaginal swab samples were collected, and the 16 s rRNA gene amplicon high-throughput sequencing technique was applied to identify the microbial composition in the samples. A similar microbial composition pattern was found between endometrial and vaginal samples, which indicated the impact of the vaginal microbiome on the endometrial microbial environment and health. There was a significant difference of endometrial and vaginal swab samples between the two groups. and were significantly more abundant in endometrial and vaginal microbiomes of group E than in group C. Their dominance was consistent with increased anaerobic bacterial taxa in the functional analysis, which might be associated with the pathogenesis of endometritis in donkeys. , a bacterial family reported in bovine semen, was statistically more abundant in endometrial microbiome of group E than in group C, which might suggest an association between high abundance of possibly due to uncleared semen and donkey endometritis. Our study revealed the composition of the vaginal and endometrial microbiomes in healthy and endometritis donkeys. These findings will provide more insights into the pathogenesis of donkey endometritis.
Copyright © 2022 Li, Zhu, Mi, Zhao, Holyoak, Yi, Wu, Wang and Zeng.
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Publication Date: 2022-08-01 PubMed ID: 35979491PubMed Central: PMC9376452DOI: 10.3389/fmicb.2022.884574Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aimed to study the differences in the vaginal and endometrial microbiomes (community of microorganisms) in healthy donkeys and those suffering from endometritis, a disease causing infertility.

Research Purpose and Methodology

  • The main purpose of this study was to analyze and compare the composition of the endometrial and vaginal microbiomes in healthy donkeys (group C) and those showing symptoms of endometritis (group E), a common cause of infertility in these animals.
  • For the research, the scientists used endometrial and vaginal swab samples collected from the donkeys.
  • To identify the microbial composition in these samples, they used a method known as the 16 s rRNA gene amplicon high-throughput sequencing technique.

Key Findings

  • The researchers noted a parallel microbial pattern in both the endometrial and vaginal samples, suggesting the important role of the vaginal microbiome in determining the microbial environment of the endometrium and in overall health.
  • There was a marked difference between the composition of the endometrial and vaginal samples taken from the two different groups (healthy donkeys and those affected by endometritis).
  • Particular types of anaerobic bacteria, which thrive without oxygen, were found significantly more in both the endometrial and vaginal microbiomes of group E (donkeys with endometritis) than in group C (healthy donkeys).
  • This dominance of anaerobic bacteria aligns with the functional analysis the researchers conducted, suggesting these bacteria might be linked to the pathogenesis, or development, of endometritis in donkeys.
  • The research also found that a particular type of bacteria, previously linked with bovine semen, was statistically more common in the endometrial microbiome of donkeys with endometritis. Researchers connected its presence to uncleared semen, hinting at potential causality between the bacteria and the condition.

Implications of the Research

  • This study offers a deeper understanding of the composition of both the vaginal and endometrial microbiomes in both healthy and diseased donkeys.
  • By identifying the potential link between the presence of certain bacteria and the development of endometritis, the findings pave the way for future research and investigation into the pathogenesis of the disease, as well as potential treatments for donkeys dealing with this form of infertility.

Cite This Article

APA
Li J, Zhu Y, Mi J, Zhao Y, Holyoak GR, Yi Z, Wu R, Wang Z, Zeng S. (2022). Endometrial and vaginal microbiome in donkeys with and without clinical endometritis. Front Microbiol, 13, 884574. https://doi.org/10.3389/fmicb.2022.884574

Publication

Frontiers in microbiology
ISSN: 1664-302X
NlmUniqueID: 101548977
Country: Switzerland
Language: English
Volume: 13
Pages: 884574
PII: 884574

Researcher Affiliations

Li, Jing
  • Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
  • National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Zhu, Yiping
  • Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Mi, Junpeng
  • School of Veterinary Science, University of Sydney, Sydney, NSW, Australia.
Zhao, Yufei
  • Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Holyoak, Gilbert Reed
  • College of Veterinary Medicine, Oklahoma State University, Stillwater, OK, United States.
Yi, Ziwen
  • Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Wu, Rongzheng
  • Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Wang, Zixuan
  • College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States.
Zeng, Shenming
  • National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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