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Endothelial glycocalyx degradation in critically ill foals.
Abstract: Endothelial glycocalyx (EG) degradation occurs in septic humans and EG products can be used as biomarkers of endothelial injury. Information about EG biomarkers and their association with disease severity is lacking in hospitalized foals. Objective: Measure serum syndecan-1 (SDC-1), heparan sulfate (HS), angiopoietin-2 (ANG-2), aldosterone (ALD), and plasma atrial natriuretic peptide (ANP) concentrations and to determine their association with disease severity and death in hospitalized foals. Methods: Ninety foals ≤3 days old. Methods: Prospective, multicenter, longitudinal study. Foals were categorized into hospitalized (n = 74; 55 septic; 19 sick nonseptic) and 16 healthy foals. Serum ([SDC-1], [HS], [ANG-2], [ALD]) and plasma (ANP) were measured over 72 hours using immunoassays. Results: Serum ([SDC-1], [HS], [ANG-2], [ALD]) and plasma (ANP) were significantly higher in hospitalized and septic than healthy foals (P < .05). Serum (ANG-2) and plasma (ANP) were significantly higher in hospitalized nonsurvivors than in survivors (P < .05). On admission, hospitalized foals with serum (HS) > 58.7 ng/mL had higher odds of nonsurvival (odds ratio [OR] = 6.1; 95% confidence interval [CI] = 1.02-36.7). Plasma (ANP) >11.5 pg/mL was associated with the likelihood of nonsurvival in hospitalized foals (OR = 7.2; 95% CI = 1.4-37.4; P < .05). Septic foals with serum (ANG-2) >1018 pg/mL on admission had higher odds of nonsurvival (OR = 6.5; 95% CI =1.2-36.6; P < .05). Conclusions: Critical illness in newborn foals is associated with EG degradation and injury, and these biomarkers are related to the severity of disease on admission and the outcome of sick foals.
© 2024 The Author(s). Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
Publication Date: 2024-09-14 PubMed ID: 39275920PubMed Central: PMC11423458DOI: 10.1111/jvim.17196Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Multicenter Study
- Observational Study
- Veterinary
Summary
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Overview
- This study investigates the degradation of the endothelial glycocalyx (EG) in critically ill newborn foals and examines several blood biomarkers related to EG injury, assessing their associations with disease severity and survival outcomes.
Background
- The endothelial glycocalyx (EG) is a protective layer lining blood vessels, playing a key role in vascular health.
- In human sepsis, EG degradation occurs and fragments released during this damage can serve as biomarkers indicating endothelial injury.
- There is limited knowledge about EG degradation biomarkers in foals, particularly how these biomarkers relate to disease severity and survival in hospitalized foals.
Objectives of the Study
- To measure concentrations of specific biomarkers in the blood of newborn foals, namely:
- Syndecan-1 (SDC-1)
- Heparan sulfate (HS)
- Angiopoietin-2 (ANG-2)
- Aldosterone (ALD)
- Atrial natriuretic peptide (ANP)
- To determine associations between these biomarkers and:
- The severity of illness in foals
- The likelihood of survival or death
Study Design and Methods
- Ninety foals aged 3 days or younger participated in a prospective, multicenter, longitudinal study.
- Foals were grouped into:
- Hospitalized foals (n=74), which included:
- Septic foals (n=55)
- Sick, nonseptic foals (n=19)
- Healthy foals (n=16)
- Blood samples were collected and analyzed over 72 hours using immunoassays to measure:
- Serum levels of SDC-1, HS, ANG-2, ALD
- Plasma levels of ANP
Key Results
- Biomarker Levels:
- Hospitalized and septic foals had significantly higher serum levels of SDC-1, HS, ANG-2, ALD, and plasma ANP compared to healthy foals (P < .05).
- Association with Survival:
- Hospitalized foals that did not survive had significantly higher serum ANG-2 and plasma ANP compared to survivors (P < .05).
- On admission, foals with serum HS levels above 58.7 ng/mL had 6.1 times higher odds of not surviving (95% CI: 1.02-36.7).
- Foals with plasma ANP levels above 11.5 pg/mL had 7.2 times higher odds of nonsurvival (95% CI: 1.4-37.4; P<.05).
- Among septic foals, those with serum ANG-2 levels over 1018 pg/mL had 6.5 times higher odds of dying (95% CI: 1.2-36.6; P<.05).
Conclusions and Implications
- Endothelial glycocalyx degradation occurs in critically ill newborn foals, particularly those hospitalized with sepsis.
- The elevated concentrations of EG biomarkers are correlated with increased disease severity and a higher risk of death.
- These biomarkers (SDC-1, HS, ANG-2, ALD, ANP) can be used to evaluate endothelial injury in foals and may serve as important tools for prognosis.
- Monitoring these biomarkers on admission could help veterinarians identify foals at greater risk and guide therapeutic decision-making.
Significance of the Study
- This study adds valuable knowledge about EG degradation in veterinary medicine, specifically in neonatal foals.
- It provides baseline data for biomarker levels related to EG injury in healthy versus critically ill foals.
- It highlights the potential for these biomarkers to predict outcomes, which can improve clinical management of foal sepsis and other critical illnesses.
Cite This Article
APA
Gomez DE, Kamr A, Gilsenan WF, Burns TA, Mudge MC, Hostnik LD, Toribio RE.
(2024).
Endothelial glycocalyx degradation in critically ill foals.
J Vet Intern Med, 38(5), 2748-2757.
https://doi.org/10.1111/jvim.17196 Publication
Researcher Affiliations
- Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
- Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
- Rood and Riddle Equine Hospital, Lexington, Kentucky, USA.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
MeSH Terms
- Animals
- Female
- Male
- Angiopoietin-2 / blood
- Animals, Newborn / blood
- Atrial Natriuretic Factor / blood
- Biomarkers / blood
- Critical Illness
- Glycocalyx / metabolism
- Heparan Sulfate / blood
- Horse Diseases / blood
- Horse Diseases / mortality
- Horse Diseases / metabolism
- Horses
- Longitudinal Studies
- Prospective Studies
- Sepsis / veterinary
- Sepsis / blood
- Sepsis / mortality
- Syndecan-1 / blood
Grant Funding
- 055052 / Equine Guelph
Conflict of Interest Statement
Authors declare no conflict of interest.
References
This article includes 51 references
- Reitsma S, Slaaf DW, Vink H, van Zandvoort MAMJ, oude Egbrink MGA. The endothelial glycocalyx: composition, functions, and visualization.. Pflugers Arch 2007;454(3):345‐359.
- Dunkel B. Science‐in‐brief: the role of the glycocalyx in critically ill patients with reference to the horse.. Equine Vet J 2020;52:790‐793.
- Lukasz A, Hillgruber C, Oberleithner H. Endothelial glycocalyx breakdown is mediated by angiopoietin‐2.. Cardiovasc Res 2017;113(6):671‐680.
- Chappell D, Bruegger D, Potzel J. Hypervolemia increases release of atrial natriuretic peptide and shedding of the endothelial glycocalyx.. Crit Care 2014;18(5):538.
- Bruegger D, Jacob M, Rehm M. Atrial natriuretic peptide induces shedding of endothelial glycocalyx in coronary vascular bed of Guinea pig hearts.. Am J Physiol Heart Circ Physiol 2005;289(5):H1993‐H1999.
- Lupu F, Kinasewitz G, Dormer K. The role of endothelial shear stress on haemodynamics, inflammation, coagulation and glycocalyx during sepsis.. J Cell Mol Med 2020;24(21):12258‐12271.
- Chalkias A. Shear stress and endothelial mechanotransduction in trauma patients with hemorrhagic shock: hidden coagulopathy pathways and novel therapeutic strategies.. Int J Mol Sci 2023;24(24):17522.
- Vittum Z, Cocchiaro S, Mensah SA. Basal endothelial glycocalyx's response to shear stress: a review of structure, function, and clinical implications.. Front Cell Dev Biol 2024;12:1371769.
- Rubanyi GM, Romero JC, Vanhoutte PM. Flow‐induced release of endothelium‐derived relaxing factor.. Am J Physiol 1986;250(6 Pt 2):H1145‐H1149.
- Burke‐Gaffney A, Evans TW. Lest we forget the endothelial glycocalyx in sepsis.. Crit Care 2012;16(2):121.
- Pillinger NL, Kam P. Endothelial glycocalyx: basic science and clinical implications.. Anaesth Intensive Care 2017;45(3):295‐307.
- Yilmaz O, Afsar B, Ortiz A, Kanbay M. The role of endothelial glycocalyx in health and disease.. Clin Kidney J 2019;12(5):611‐619.
- Schött U, Solomon C, Fries D, Bentzer P. The endothelial glycocalyx and its disruption, protection and regeneration: a narrative review.. Scand J Trauma Resusc Emerg Med 2016;24:48.
- Hirota T, Levy JH, Iba T. The influence of hyperglycemia on neutrophil extracellular trap formation and endothelial glycocalyx damage in a mouse model of type 2 diabetes.. Microcirculation 2020;27(5):e12617.
- Molema G, Zijlstra JG, van Meurs M, Kamps JAAM. Renal microvascular endothelial cell responses in sepsis‐induced acute kidney injury.. Nat Rev Nephrol 2022;18(2):95‐112.
- Selim J, Hamzaoui M, Boukhalfa I. Cardiopulmonary bypass increases endothelial dysfunction after pulmonary ischaemia‐reperfusion in an animal model.. Eur J Cardiothorac Surg 2021;59(5):1037‐1047.
- Martin JV, Liberati DM, Diebel LN. Excess sodium is deleterious on endothelial and glycocalyx barrier function: a microfluidic study.. J Trauma Acute Care Surg 2018;85(1):128‐134.
- Hobbs KJ, Johnson PJ, Wiedmeyer CE, Schultz L, Foote CA. Plasma syndecan‐1 concentration as a biomarker for endothelial glycocalyx degradation in septic adult horses.. Equine Vet J 2023;55(3):456‐462.
- Gomez DE, Biermann NM, Sanchez LC. Physicochemical approach to determine the mechanism for acid‐base disorders in 793 hospitalized foals.. J Vet Intern Med 2015;29(5):1395‐1402.
- Rings LM, Kamr AM, Kinsella HM. The enteroinsular axis during hospitalization in newborn foals.. Domest Anim Endocrinol 2022;78:106686.
- Dembek KA, Onasch K, Hurcombe SDA. Renin‐angiotensin‐aldosterone system and hypothalamic‐pituitary‐adrenal axis in hospitalized newborn foals.. J Vet Intern Med 2013;27(2):331‐338.
- Oberleithner H, Riethmüller C, Schillers H, MacGregor GA, de Wardener HE, Hausberg M. Plasma sodium stiffens vascular endothelium and reduces nitric oxide release.. Proc Natl Acad Sci U S A 2007;104(41):16281‐16286.
- Dembek KA, Hurcombe SD, Stewart AJ. Association of aldosterone and arginine vasopressin concentrations and clinical markers of hypoperfusion in neonatal foals.. Equine Vet J 2016;48(2):176‐181.
- Brewer BD, Koterba AM. Development of a scoring system for the early diagnosis of equine neonatal sepsis.. Equine Vet J 1988;20(1):18‐22.
- Trachsel DS, Schwarzwald CC, Grenacher B, Weishaupt MA. Analytic validation and comparison of three commercial immunoassays for measurement of plasma atrial/A‐type natriuretic peptide concentration in horses.. Res Vet Sci 2014;96(1):180‐186.
- Rezazadeh F. Measurement of biomarkers troponin I, NT pro BNP and ANP between horses with signs of respiratory disease and apparently healthy group.. Anim Vet Sci 2016;4(6):86.
- Yagmur E, Koch A, Haumann M, Kramann R, Trautwein C, Tacke F. Hyaluronan serum concentrations are elevated in critically ill patients and associated with disease severity.. Clin Biochem 2012;45(1–2):82‐87.
- Smart L, Macdonald SPJ, Burrows S, Bosio E, Arendts G, Fatovich DM. Endothelial glycocalyx biomarkers increase in patients with infection during Emergency Department treatment.. J Crit Care 2017;42:304‐309.
- Jiménez M, Cervantes‐García D, Córdova‐Dávalos LE, Pérez‐Rodríguez MJ, Gonzalez‐Espinosa C, Salinas E. Responses of mast cells to pathogens: beneficial and detrimental roles.. Front Immunol 2021;12:685865.
- Okada H, Takemura G, Suzuki K. Three‐dimensional ultrastructure of capillary endothelial glycocalyx under normal and experimental endotoxemic conditions.. Crit Care 2017;21(1):261.
- Sieve I, Münster‐Kühnel AK, Hilfiker‐Kleiner D. Regulation and function of endothelial glycocalyx layer in vascular diseases.. Vascul Pharmacol 2018;100:26‐33.
- Abou‐Arab O, Kamel S, Beyls C. Vasoplegia after cardiac surgery is associated with endothelial glycocalyx alterations.. J Cardiothorac Vasc Anesth 2020;34(4):900‐905.
- Suri C, Jones PF, Patan S. Requisite role of angiopoietin‐1, a ligand for the TIE2 receptor, during embryonic angiogenesis.. Cell 1996;87(7):1171‐1180.
- Salmon AHJ, Neal CR, Sage LM, Glass CA, Harper SJ, Bates DO. Angiopoietin‐1 alters microvascular permeability coefficients in vivo via modification of endothelial glycocalyx.. Cardiovasc Res 2009;83(1):24‐33.
- Parikh SM, Mammoto T, Schultz A. Excess circulating angiopoietin‐2 may contribute to pulmonary vascular leak in sepsis in humans.. PLoS Med 2006;3(3):e46.
- Wright JK, Hayford K, Tran V. Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched case‐control study.. BMC Pediatr 2018;18(1):118.
- Liu XW, Ma T, Liu W. Sustained increase in angiopoietin‐2, heparin‐binding protein, and procalcitonin is associated with severe sepsis.. J Crit Care 2018;45:14‐19.
- Yu WK, McNeil JB, Wickersham NE, Shaver CM, Bastarache JA, Ware LB. Angiopoietin‐2 outperforms other endothelial biomarkers associated with severe acute kidney injury in patients with severe sepsis and respiratory failure.. Crit Care 2021;25(1):48.
- Jacob M, Saller T, Chappell D, Rehm M, Welsch U, Becker BF. Physiological levels of A‐, B‐ and C‐type natriuretic peptide shed the endothelial glycocalyx and enhance vascular permeability.. Basic Res Cardiol 2013;108(3):347.
- Witthaut R, Busch C, Fraunberger P. Plasma atrial natriuretic peptide and brain natriuretic peptide are increased in septic shock: impact of interleukin‐6 and sepsis‐associated left ventricular dysfunction.. Intensive Care Med 2003;29(10):1696‐1702.
- Crompton M, Skinner LJ, Satchell SC, Butler MJ. Aldosterone: essential for life but damaging to the vascular endothelium.. Biomolecules 2023;13(6):1004.
- Stratta P, Canavese C, Gurioli L. Ratio between aldosterone and atrial natriuretic peptide in pregnancy.. Kidney Int 1989;36(5):908‐914.
- Saoraya J, Wongsamita L, Srisawat N, Musikatavorn K. Plasma syndecan‐1 is associated with fluid requirements and clinical outcomes in emergency department patients with sepsis.. Am J Emerg Med 2021;42:83‐89.
- Hippensteel JA, Uchimido R, Tyler PD. Intravenous fluid resuscitation is associated with septic endothelial glycocalyx degradation.. Crit Care 2019;23(1):259.
- Anand D, Ray S, Srivastava LM, Bhargava S. Evolution of serum hyaluronan and syndecan levels in prognosis of sepsis patients.. Clin Biochem 2016;49(10–11):768‐776.
- Huang X, Hu H, Sun T. Plasma endothelial glycocalyx components as a potential biomarker for predicting the development of disseminated intravascular coagulation in patients with sepsis.. J Intensive Care Med 2021;36(11):1286‐1295.
- Hahn RG, Zdolsek M, Zdolsek J. Plasma concentrations of syndecan‐1 are dependent on kidney function.. Acta Anaesthesiol Scand 2021;65(6):809‐815.
- Nelson A, Berkestedt I, Schmidtchen A, Ljunggren L, Bodelsson M. Increased levels of glycosaminoglycans during septic shock: relation to mortality and the antibacterial actions of plasma.. Shock 2008;30(6):623‐627.
- Johansson PI, Bergström A, Aachmann‐Andersen NJ. Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans.. Front Physiol 2014;5:459.
- Abassi Z, Armaly Z, Heyman SN. Glycocalyx degradation in ischemia‐reperfusion injury.. Am J Pathol 2020;190(4):752‐767.
- Bruegger D, Brettner F, Rossberg I. Acute degradation of the endothelial glycocalyx in infants undergoing cardiac surgical procedures.. Ann Thorac Surg 2015;99(3):926‐931.
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