Enterocytozoon bieneusi Genotypes in Grazing Horses in China and their Zoonotic Transmission Potential.
Abstract: In present study, 262 fecal specimens were collected from 12 groups of grazing horses in the Xinjiang Uyghur Autonomous Region of China. The specimens were subjected to PCR and sequencing analyses of the ribosomal internal transcribed spacer (ITS). The overall prevalence of E. bieneusi in horses was 30.9% (81/262). No significant differences in prevalence were observed between horses of different ages or sexes. Nineteen genotypes were identified: 15 known genotypes (BEB6, CHG19, CM6, CM7, CM8, CS-1, CS-4, D, EpbA, EbpC, G, horse1, horse2, O, and Peru8) and four new genotypes (XJH1-XJH4). Six of these genotypes were previously detected in humans: BEB6, D, EbpA, EbpC, O, and Peru8. Genotype EbpC was the most prevalent (21/81), followed by EpbA (20/81), BEB6 (9/81), CM6 (4/81), horse1 (4/81), O (4/81), G (3/81), CHG19 (2/81), CM7 (2/81), horse2 (2/81), and XJH1 (2/81), whereas the remaining eight genotypes were seen in one specimen each. In a phylogenetic analysis, 14 genotypes (65/81, 80.2%), excluding genotypes BEB6, CM7, horse2, XJH1, and XJH4, belonged to group 1, which have zoonotic potential. The high diversity in the E. bieneusi genotypes and their zoonotic potential suggest that grazing horses are a potential source of zoonotic infection in humans.
© 2016 The Author(s) Journal of Eukaryotic Microbiology © 2016 International Society of Protistologists.
Publication Date: 2016-03-08 PubMed ID: 26909747DOI: 10.1111/jeu.12308Google Scholar: Lookup
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Summary
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The research article focuses on the investigation of Enterocytozoon bieneusi genotypes in grazing horses in China, their prevalence, and potential for zoonotic transmission.
Sample Collection and Analysis
- 262 fecal samples were collected from 12 groups of grazing horses in the Xinjiang Uyghur Autonomous Region of China.
- The researchers utilized PCR (polymerase chain reaction) and sequencing analysis techniques on the ribosomal internal transcribed spacer (ITS) within the samples. This method enabled them to detect and identify specific genotypes of E. bieneusi.
Prevalence of E. bieneusi
- The prevalence of E. bieneusi was found to be 30.9%; 81 out of 262 horse samples were found to contain the parasite.
- The prevalence did not significantly vary based on age or sex of the horses.
Genotype Identification
- 19 different genotypes were identified among the positive samples. These included 15 known genotypes and four newly discovered ones, named XJH1 through XJH4.
- Of these genotypes, six were previously identified in human hosts: BEB6, D, EbpA, EbpC, O, and Peru8.
- The most prevalent genotype was EbpC, followed by EpbA and BEB6. The newly discovered genotype XJH1 also showed up multiple times, while the other three newly identified genotypes occurred just once each in the samples.
Phylogenetic Analysis and Zoonotic Potential
- Phylogenetic analysis helped researchers map the genetic relationships between the identified genotypes.
- 80.2% of the genotypes (14 genotypes identified in 65 out of 81 positive samples), excluding genotypes BEB6, CM7, horse2, XJH1, and XJH4, were placed in group 1, a classification that denotes potential for zoonotic disease transfer.
- This diversity of genotypes and their potential zoonotic transmission suggest that grazing horses can potentially be a source of E. bieneusi infection in humans.
Cite This Article
APA
Qi M, Wang R, Wang H, Jian F, Li J, Zhao J, Dong H, Zhu H, Ning C, Zhang L.
(2016).
Enterocytozoon bieneusi Genotypes in Grazing Horses in China and their Zoonotic Transmission Potential.
J Eukaryot Microbiol, 63(5), 591-597.
https://doi.org/10.1111/jeu.12308 Publication
Researcher Affiliations
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- College of Animal Science, Tarim University, 1487 Tarim Road, Alar, 843300, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu, 451450, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
MeSH Terms
- Age Factors
- Animals
- Base Sequence
- Biodiversity
- China / epidemiology
- DNA, Fungal / genetics
- DNA, Ribosomal Spacer / genetics
- Enterocytozoon / classification
- Enterocytozoon / genetics
- Enterocytozoon / pathogenicity
- Feces / parasitology
- Genotype
- Horses / parasitology
- Humans
- Microsporidiosis / epidemiology
- Microsporidiosis / parasitology
- Microsporidiosis / transmission
- Microsporidiosis / veterinary
- Phylogeny
- Polymerase Chain Reaction / veterinary
- Prevalence
- Sequence Analysis, DNA
- Sex Factors
- Zoonoses / epidemiology
- Zoonoses / parasitology
- Zoonoses / transmission
Citations
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