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Home / Equine Research Bank / BMC Vet Res / Enterocytozoon bieneusi in donkeys from Xinjiang, China: pre...
BMC veterinary research2020; 16(1); 196; doi: 10.1186/s12917-020-02409-0

Enterocytozoon bieneusi in donkeys from Xinjiang, China: prevalence, molecular characterization and the assessment of zoonotic risk.

Abstract: Enterocytozoon bieneusi, a zoonotic pathogen, has the potential to infect both immunocompromised and immunocompetent humans. It is found in large number of animals; however, not much is known regarding its prevalence in equine animals, particularly donkeys. This is the first molecular epidemiological evaluation of E. bieneusi in 178 free-ranging donkeys from five countrysides; and 502 farmed donkeys from 18 farms in 12 cities of Xinjiang, China by Nested PCR. Results: E. bieneusi was detected in 2.5% (17/680) donkeys, with 2.6% (13/502) in farmed and 2.2% (4/178) in free-ranging ones. Sequence analysis identified eight ITS genotypes, all belonging to zoonotic Groups 1 or 2, including six known genotypes: horse1 (n = 5), D (n = 3), NCD-2 (n = 3), BEB6 (n = 2), BEB4 (n = 1), and NIAI (n = 1); and two new genotypes: XJD1 (n = 1) and XJD2 (n = 1). Conclusions: This is the first report confirming the presence of E. bieneusi in donkeys in Xinjiang, China, and indicates the possibility of zoonotic transmission of this pathogenic parasite.
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Publication Date: 2020-06-15 PubMed ID: 32576195PubMed Central: PMC7313214DOI: 10.1186/s12917-020-02409-0Google Scholar: Lookup
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Summary

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This research paper investigates the prevalence and characteristics of the zoonotic pathogen Enterocytozoon bieneusi in donkeys in Xinjiang, China and explores the potential for zoonotic transmission.

Research Context and Methodology

  • The study focuses on Enterocytozoon bieneusi, a zoonotic pathogen that can infect both individuals with compromised and healthy immune systems. Though its presence in a variety of animal species has been documented, its prevalence in equine species such as donkeys is less studied.
  • A molecular epidemiological evaluation of E. bieneusi in donkeys from Xinjiang, China was conducted for the first time. The study sample included 178 free-ranging donkeys from five countrysides and 502 farmed donkeys from 18 different farms in 12 different cities.
  • The Nested PCR technique was utilized to detect the presence of E. bieneusi in these donkeys.

Findings of the Research

  • The research identified E. bieneusi in 2.5% of tested donkeys, with slightly higher prevalence in farmed than free-ranging ones.
  • Through sequence analysis, eight distinct types of the pathogen were identified. All of these types belonged to the zoonotic Groups 1 or 2, meaning they have the potential to be transmitted to humans.
  • Of these eight identified types, six were recognized from previous research: horse1, D, NCD-2, BEB6, BEB4, and NIAI.
  • In addition to these known genotypes, two new ones were found, labeled as XJD1 and XJD2.

Significance and Conclusions

  • This research provides new insight into the occurrence and genetic diversity of E. bieneusi in donkeys in Xinjiang, China – a topic previously unexplored. This is the first study confirming the presence of this pathogenic parasite in donkeys in this region.
  • The identification of eight distinct genotypes, all falling under two zoonotic groups, underlines the risk of zoonotic transmission, reinforcing the need for further research and potential mitigating measures to prevent such transmission to humans.

Cite This Article

APA
Zhao A, Zhang Y, Wang W, Jing B, Xing J, Tao D, Zhao W, Qi M. (2020). Enterocytozoon bieneusi in donkeys from Xinjiang, China: prevalence, molecular characterization and the assessment of zoonotic risk. BMC Vet Res, 16(1), 196. https://doi.org/10.1186/s12917-020-02409-0

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 16
Issue: 1
Pages: 196
PII: 196

Researcher Affiliations

Zhao, Aiyun
  • College of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, China.
Zhang, Ying
  • College of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, China.
Wang, Wen
  • College of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, China.
Jing, Bo
  • College of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, China.
Xing, Jinming
  • College of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, China.
Tao, Dayong
  • College of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, China.
Zhao, Wei
  • Department of Parasitology, Wenzhou Medical University, Wenzhou, 325035, Zhejiang Province, China. hayidazhaowei@163.com.
Qi, Meng
  • College of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, China. qimengdz@163.com.

MeSH Terms

  • Animals
  • China / epidemiology
  • DNA, Fungal / genetics
  • Enterocytozoon / classification
  • Enterocytozoon / genetics
  • Enterocytozoon / isolation & purification
  • Equidae / microbiology
  • Feces / microbiology
  • Genotype
  • Microsporidiosis / epidemiology
  • Microsporidiosis / veterinary
  • Polymerase Chain Reaction / veterinary
  • Prevalence
  • Sequence Analysis, DNA / veterinary
  • Zoonoses / epidemiology
  • Zoonoses / microbiology

Grant Funding

  • 31660712 / National Natural Science Foundation of China
  • 31860699 / National Natural Science Foundation of China

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Citations

This article has been cited 1 times.
  1. Abarca N, Santín M, Ortega S, Maloney JG, George NS, Molokin A, Cardona GA, Dashti A, Köster PC, Bailo B, Hernández-de-Mingo M, Muadica AS, Calero-Bernal R, Carmena D, González-Barrio D. Molecular Detection and Characterization of Blastocystis sp. and Enterocytozoon bieneusi in Cattle in Northern Spain.. Vet Sci 2021 Sep 11;8(9).
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