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Applied and environmental microbiology2001; 67(12); 5551-5557; doi: 10.1128/AEM.67.12.5551-5557.2001

Enterotoxigenic potential of Staphylococcus intermedius.

Abstract: Staphylococcal food poisoning (SFP) caused by enterotoxigenic staphylococci is one of the main food-borne diseases. In contrast to Staphylococcus aureus, a systematic screening for the enterotoxins has not yet been performed on the genomic level for the coagulase-positive species S. intermedius. Therefore, the enterotoxigenic potential of 281 different veterinary (canine, n = 247; equine, n = 23; feline, n = 9; other, n = 2) and 11 human isolates of S. intermedius was tested by using a multiplex PCR DNA-enzyme immunoassay system targeting the staphylococcal enterotoxin genes sea, seb, sec, sed, and see. Molecular results were compared by in vitro testing of enterotoxin production by two immunoassays. A total of 33 (11.3%) S. intermedius isolates, including 31 (12.6%) canine isolates, 1 equine isolate, and 1 human isolate, tested positive for the sec gene. In vitro production of the respective enterotoxins was detected in 30 (90.9%) of these isolates by using immunological tests. In contrast, none of 65 veterinary specimen-derived isolates additionally tested and comprising 13 (sub)species of coagulase-negative staphylococci were found to be enterotoxigenic. This study shows on both molecular and immunological levels that a substantial number of S. intermedius isolates harbor the potential for enterotoxin production. Since evidence for noninvasive zoonotic transmission of S. intermedius from animal hosts to humans has been documented, an enterotoxigenic role of this microorganism in SFP via contamination of food products may be assumed.
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Publication Date: 2001-11-28 PubMed ID: 11722906PubMed Central: PMC93343DOI: 10.1128/AEM.67.12.5551-5557.2001Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the potential of Staphylococcus intermedius, a bacterium often found in animals, to produce enterotoxins that can cause food poisoning in humans. Through molecular and immunological testing of 281 veterinary and 11 human isolates, it was found that a significant number of these samples had the potential to produce harmful enterotoxins.

Study Overview

  • The research involved testing the enterotoxigenic potential of 281 veterinary (mainly from dogs but also horses, cats, and others) and 11 human isolates of Staphylococcus intermedius. This bacterium is commonly associated with animals, particularly dogs, but it is also known to occasionally cause infections in humans.
  • The testing was done using a multiplex PCR DNA-enzyme immunoassay that targeted specific enterotoxin genes — sea, seb, sec, sed, and see. These genes are associated with the production of enterotoxins, which are toxins that cause food poisoning when ingested.
  • In addition to the molecular testing, the team also conducted in vitro testing of enterotoxin production using two immunoassays. This was done to compare the results with the molecular tests.

Key Findings

  • Of the Staphylococcus intermedius isolates tested, 33 (11.3%) were found to contain the sec gene, an enterotoxin gene. The detection of an enterotoxin gene in these isolates suggests they have the potential to produce toxins that cause food poisoning.
  • The sec gene was found in a larger proportion of canine isolates (12.6%) compared to other species. It was also found in one equine isolate and one human isolate.
  • Immunological tests confirmed the production of enterotoxins in 90.9% of the positive isolates, thus validating the molecular results.
  • None of the additional 65 veterinary isolates from different species of coagulase-negative staphylococci were found to be enterotoxigenic, indicating that the enterotoxin-producing potential may be specific to S. intermedius among the tested bacteria.

Implications and Conclusions

  • The findings of this study show that S. intermedius has a substantial potential to produce enterotoxins that can cause food poisoning in humans.
  • Given the documented cases of S. intermedius transmission from animal to humans, it is suggested that food contamination by this bacterium could potentially lead to staphylococcal food poisoning.
  • Despite the prevalence of S. intermedius being largely associated with animals, its potential as a human pathogen due to its enterotoxigenic properties is significant and warrants further investigation.

Cite This Article

APA
Becker K, Keller B, von Eiff C, Brück M, Lubritz G, Etienne J, Peters G. (2001). Enterotoxigenic potential of Staphylococcus intermedius. Appl Environ Microbiol, 67(12), 5551-5557. https://doi.org/10.1128/AEM.67.12.5551-5557.2001

Publication

Applied and environmental microbiology
ISSN: 0099-2240
NlmUniqueID: 7605801
Country: United States
Language: English
Volume: 67
Issue: 12
Pages: 5551-5557

Researcher Affiliations

Becker, K
  • Institute of Medical Microbiology, University of Münster, 48149 Münster, Germany. kbecker@uni-muenster.de
Keller, B
    von Eiff, C
      Brück, M
        Lubritz, G
          Etienne, J
            Peters, G

              MeSH Terms

              • Animals
              • Base Sequence
              • Cat Diseases / microbiology
              • Cats
              • Dog Diseases / microbiology
              • Dogs
              • Enterotoxins / genetics
              • Enterotoxins / metabolism
              • Guinea Pigs
              • Horse Diseases / microbiology
              • Horses
              • Humans
              • Immunoenzyme Techniques
              • Molecular Sequence Data
              • Polymerase Chain Reaction
              • Rabbits
              • Staphylococcal Infections / microbiology
              • Staphylococcal Infections / veterinary
              • Staphylococcus / classification
              • Staphylococcus / genetics
              • Staphylococcus / isolation & purification
              • Staphylococcus / metabolism

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