Enzyme-linked immunosorbent assay for serological survey of equine arteritis virus in racehorses.
Abstract: To examine antibodies against equine arteritis virus (EAV), an enzyme-linked immunosorbent assay (ELISA) using purified virus antigen was developed. The results of ELISA were compared with those of serum neutralization (SN) tests. The ELISA absorbance values and the SN titers in sera collected weekly from EAV-infected horses showed a similar pattern. The ELISA could detect antibody to EAV in horses experimentally infected with not only a homologous virus strain, which was used as the ELISA antigen, but also a heterologous strain. Using the ELISA, serum samples collected in 1996 from racehorses in three prefectures (Hokkaido, Ibaraki, and Shiga) were examined and there was no evidence of recent EAV infection among these racehorse populations in Japan. The ELISA should be a simple and highly specific method for rapid screening of EAV infection in racehorses.
Publication Date: 1998-10-31 PubMed ID: 9795910DOI: 10.1292/jvms.60.1043Google Scholar: Lookup
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- Journal Article
- Adult Horses
- Antibodies
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Equine Viral Arteritis
- Horses
- Immunology
- Infection
- Infectious Disease
- Racehorses
- Serodiagnosis
- Serological Surveys
- Seroprevalence
- Veterinary Medicine
- Veterinary Research
- Virus
Summary
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This research article reports on a study that developed an enzyme-linked immunosorbent assay (ELISA) to test for antibodies against equine arteritis virus (EAV) in racehorses. The test was found to be effective for rapid screening of EAV infection in racehorses.
Development of the ELISA Test
- The researchers successfully developed an enzyme-linked immunosorbent assay (ELISA) using a purified virus antigen. This assay was specifically designed to test for antibodies against equine arteritis virus (EAV).
- Antigen used in the ELISA was derived from a virus strain specifically selected to test for EAV.
- The ELISA is a test that measures the strength of an immune response in the body – in this case, it tested for the presence of antibodies produced in response to an EAV infection.
Comparative Analysis with Neutralization Tests
- The ELISA results were compared with those of serum neutralization (SN) tests, which are a type of immunoassay that measure the ability of an organism’s immune system to neutralize a virus.
- The study found that the ELISA absorbance values and the SN titers in sera collected weekly from EAV-infected horses showed a similar pattern, indicating the reliability and efficacy of the developed ELISA test.
Testing Across Different Virus Strains
- The ELISA was able to detect EAV antibodies not only in horses infected with the homologous virus strain (from the same species), but also in those infected with a heterologous strain (from a different species).
- This proves the versatility and wide-reaching application of the developed ELISA.
Survey of EAV among Racehorse Populations in Japan
- Having established the test, the researchers then sampled serum from racehorses in three prefectures of Japan (Hokkaido, Ibaraki, and Shiga) in 1996.
- Interestingly, the ELISA found no evidence of recent EAV infections among these racehorse populations, suggesting effective disease control in these regions.
Concluding Remarks
- The development of the ELISA for EAV presents a simple, highly specific, and quick method for screening EAV infections in racehorses.
- Such a tool could be instrumental in managing and preventing disease spread in equine populations, ensuring the health and performance of racehorses.
Cite This Article
APA
Kondo T, Fukunaga Y, Sekiguchi K, Sugiura T, Imagawa H.
(1998).
Enzyme-linked immunosorbent assay for serological survey of equine arteritis virus in racehorses.
J Vet Med Sci, 60(9), 1043-1045.
https://doi.org/10.1292/jvms.60.1043 Publication
Researcher Affiliations
- Epizootic Research Station, Equine Research Institute, Japan Racing Association, Tochigi, Japan.
MeSH Terms
- Animals
- Antibodies, Viral / analysis
- Antigens, Viral / immunology
- Arterivirus Infections / immunology
- Arterivirus Infections / veterinary
- Arterivirus Infections / virology
- Enzyme-Linked Immunosorbent Assay / veterinary
- Equartevirus / immunology
- Horse Diseases / immunology
- Horse Diseases / virology
- Horses
Citations
This article has been cited 4 times.- Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kokado H, Kondo T. Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus. J Equine Sci 2018 Dec;29(4):111-115.
- Balasuriya UB, Go YY, MacLachlan NJ. Equine arteritis virus. Vet Microbiol 2013 Nov 29;167(1-2):93-122.
- Go YY, Snijder EJ, Timoney PJ, Balasuriya UB. Characterization of equine humoral antibody response to the nonstructural proteins of equine arteritis virus. Clin Vaccine Immunol 2011 Feb;18(2):268-79.
- Go YY, Wong SJ, Branscum AJ, Demarest VL, Shuck KM, Vickers ML, Zhang J, McCollum WH, Timoney PJ, Balasuriya UB. Development of a fluorescent-microsphere immunoassay for detection of antibodies specific to equine arteritis virus and comparison with the virus neutralization test. Clin Vaccine Immunol 2008 Jan;15(1):76-87.
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