Journal of equine science2018; 29(4); 111-115; doi: 10.1294/jes.29.111

Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus.

Abstract: In order to establish an efficient system for serological diagnosis of equine viral arteritis in Japan, we compared enzyme-linked immunosorbent assays (ELISAs) provided by two manufacturers (Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, U.S.A.) by testing a series of horse sera. The results revealed that 159 of 160 virus-neutralizing (VN) antibody-positive serum samples were positive in both the Nisseiken-ELISA and VMRD-ELISA. Of the VN-negative sera (n=157), 134 and 154 samples were negative in the Nisseiken-ELISA and VMRD-ELISA, respectively. Sensitivity was 99.4% for both the Nisseiken-ELISA and VMRD-ELISA. The specificity of the VMRD-ELISA (98.1%) was significantly higher than that of the Nisseiken-ELISA (85.4%, P<0.05). The diagnostic performance of the VMRD-ELISA was superior to that of the Nisseiken-ELISA because of this greater specificity.
Publication Date: 2018-12-18 PubMed ID: 30607135PubMed Central: PMC6306293DOI: 10.1294/jes.29.111Google Scholar: Lookup
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  • Journal Article

Summary

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This research article discusses a comparison study of two Enzyme-Linked Immunosorbent Assays (ELISAs), produced by different manufacturers, for their efficacy in the detection of antibodies against the equine arteritis virus. The comparable sensitivity but notably different specificity rates of the two assays were found, with the VMRD-ELISA showing superior performance.

Objective of Research

  • This study aims to establish an effective system for detecting the equine arteritis virus in Japan.
  • To achieve this, the research compares two ELISAs used to detect antibodies against the equine arteritis virus.
  • The two ELISAs in question are produced by Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, USA.

Methods Used

  • The researchers tested a number of horse serum samples using both the Nisseiken and VMRD ELISAs.
  • The number of samples that tested positive to Virus-Neutralizing (VN) antibodies in each ELISA were analyzed and recorded.

Results

  • Out of 160 VN antibody-positive serum samples, 159 tested positive in both the Nisseiken-ELISA and VMRD-ELISA.
  • Of the VN antibody-negative sera, 134 samples were negative in the Nisseiken-ELISA while a larger number (154) were negative in the VMRD-ELISA.
  • Thus, both ELISAs demonstrated a nearly perfect sensitivity rate of 99.4%.

Interpretation of Findings

  • The specificity of the two ELISAs varied significantly. The VMRD-ELISA demonstrated higher specificity at 98.1%, compared to the Nisseiken-ELISA, which only had a specificity rate of 85.4%.
  • The superior specificity of the VMRD-ELISA made it a more reliable diagnostic tool for detecting the equine arteritis virus.
  • The higher specificity of the VMRD-ELISA means it has a lower chance of giving false-positive results compared to the Nisseiken-ELISA.

Conclusion

  • Although both the Nisseiken and VMRD ELISAs demonstrated high sensitivity in detecting antibodies against the equine arteritis virus, the VMRD-ELISA had significantly higher specificity.
  • This makes the VMRD-ELISA more reliable in serological diagnosis, as it has a lower probability of giving false-positive results.
  • As such, the VMRD-ELISA could potentially offer a more reliable tool for serological diagnosis in Japan.

Cite This Article

APA
Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kokado H, Kondo T. (2018). Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus. J Equine Sci, 29(4), 111-115. https://doi.org/10.1294/jes.29.111

Publication

ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 29
Issue: 4
Pages: 111-115

Researcher Affiliations

Bannai, Hiroshi
  • Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Nemoto, Manabu
  • Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Tsujimura, Koji
  • Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Yamanaka, Takashi
  • Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Kokado, Hiroshi
  • Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Kondo, Takashi
  • Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.

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