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Home / Equine Research Bank / Animals (Basel) / Epithelial to Mesenchymal Transition (EMT) in a Laryngeal Sq...
Animals : an open access journal from MDPI2020; 10(12); 2318; doi: 10.3390/ani10122318

Epithelial to Mesenchymal Transition (EMT) in a Laryngeal Squamous Cell Carcinoma of a Horse: Future Perspectives.

Abstract: Squamous cell carcinoma (SCC) is one of the most frequent tumors of skin and muco-cutaneous junctions in the horse. Equine papillomavirus type 2 (EcPV2) has been detected in equine SCC of the oral tract and genitals, and recently also in the larynx. As human squamous cell carcinoma of the larynx (SCCL), it is strongly etiologically associated with high-risk papillomavirus (h-HPV) infection. This study focuses on tumor cells behavior in a naturally occurring tumor that can undergo the so-called epithelial to mesenchymal transition (EMT). A SCCL in a horse was investigated by immunohistochemistry using antibodies against E-cadherin, pan-cytokeratin AE3/AE1, β-catenin, N-cadherin, vimentin, ZEB-1, TWIST, and HIF-1α. EcPV2 DNA detection and expression of oncogenes in SCC were investigated. A cadherin switch and an intermediate filaments rearrangement within primary site tumor cells together with the expression of the EMT-related transcription factors TWIST-1, ZEB-1, and HIF-1α were observed. DNA obtained from the tumor showed EcPV2 positivity, with E2 gene disruption and E6 gene dysregulation. The results suggest that equine SCCL might be a valuable model for studying EMT and the potential interactions between EcPV2 oncoproteins and the EMT process in SCCL.
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Publication Date: 2020-12-07 PubMed ID: 33297475PubMed Central: PMC7762370DOI: 10.3390/ani10122318Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the behavior of tumor cells in a horse affected by Laryngeal Squamous Cell Carcinoma (SCCL). Using immunohistochemistry and DNA detection, the researchers identified changes in tumor cells and expression of certain genes that suggest this form of equine cancer may be useful in studying Epithelial to Mesenchymal Transition (EMT), a process linked to cancer progression.

Background

  • Equine Squamous Cell Carcinoma (SCC) is a common type of tumor often found in the skin and the junctions between skin and mucous membranes in horses.
  • The tumor often associates with the presence of the Equine papillomavirus type 2 (EcPV2), which is also found in similar types of cancer in humans.
  • The study focuses on a naturally occurring tumor in a horse that has exhibited epithelial to mesenchymal transition (EMT), a process in which cells change from being immobile and tightly held together, to becoming mobile and capable of invading other tissues, thus spreading the disease.

Methodology

  • The researchers examined a SCCL in a horse using immunohistochemistry, a process that involves labeling antibodies with a staining agent to detect and study specific proteins in cells.
  • The antibody staining agents used were E-cadherin, pan-cytokeratin AE3/AE1, β-catenin, N-cadherin, vimentin, ZEB-1, TWIST, and HIF-1α, each of which serves a specific purpose in studying malignant or cancerous cells.
  • The process also involved investigating the detection and expression of EcPV2 DNA and oncogenes in SCC.

Findings

  • There was observed a switch in cadherin, a family of proteins that determine cell adhesion, and an arrangement of intermediate filaments within primary tumor cells.
  • The study indicated the tumor cells expressing EMT-related transcription factors TWIST-1, ZEB-1, and HIF-1α.
  • DNA obtained from the tumor showed evidence of EcPV2, along with disruption and dysregulation of E2 and E6 genes respectively.

Implication

  • These findings suggest that equine SCCL may be a useful model for studying EMT.
  • The study indicates potential interactions between EcPV2 oncoproteins and the EMT process in SCCL, offering potential for future research in this area.

Cite This Article

APA
Armando F, Godizzi F, Razzuoli E, Leonardi F, Angelone M, Corradi A, Meloni D, Ferrari L, Passeri B. (2020). Epithelial to Mesenchymal Transition (EMT) in a Laryngeal Squamous Cell Carcinoma of a Horse: Future Perspectives. Animals (Basel), 10(12), 2318. https://doi.org/10.3390/ani10122318

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 10
Issue: 12
PII: 2318

Researcher Affiliations

Armando, Federico
  • Pathology Unit, Department of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, Italy.
Godizzi, Francesco
  • Department of Veterinary Science (DIMEVET), University of Milan, Via dell'Università 6, 26900 Lodi, Italy.
Razzuoli, Elisabetta
  • National Reference Center of Veterinary and Comparative Oncology (CEROVEC), Piazza Borgo Pila 39/24, 16129 Genoa, Italy.
Leonardi, Fabio
  • Department of Veterinary Science, Strada del Taglio 10, 43126 Parma, Italy.
Angelone, Mario
  • Department of Veterinary Science, Strada del Taglio 10, 43126 Parma, Italy.
Corradi, Attilio
  • Pathology Unit, Department of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, Italy.
Meloni, Daniela
  • National Reference Center of Veterinary and Comparative Oncology (CEROVEC), Piazza Borgo Pila 39/24, 16129 Genoa, Italy.
Ferrari, Luca
  • Pathology Unit, Department of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, Italy.
Passeri, Benedetta
  • Pathology Unit, Department of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, Italy.

Grant Funding

  • IZS PLV 15/18 RC / Italian Ministry of Health

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 7 times.
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