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Home / Equine Research Bank / Front Vet Sci / Equine adipose-derived stem cells modulate in vitro neutroph...
Frontiers in veterinary science2025; 12; 1685757; doi: 10.3389/fvets.2025.1685757

Equine adipose-derived stem cells modulate in vitro neutrophil extracellular trap release by polymorphonuclear neutrophils.

Abstract: Neutrophil extracellular trap (NET) are thin and long web-like structures composed of DNA and antimicrobial proteins released by activated polymorphonuclear neutrophils (PMN) as part of the innate immune response. Adipose-derived stem cells (ADSCs) represent an accessible, abundant and minimal invasive source of mesenchymal stem cells (MSCs), with high regenerative potential, immunomodulatory and anti-inflammatory properties. Although recognized immunomodulatory properties of ADSCs, their interaction with PMN and their role on NET formation remains poorly characterized. The present study aimed to evaluate the effects of equine ADSCs on NET formation by equine PMN. Equine ADSCs were isolated from two different sources of adipose tissue, subcutaneous and retroperitoneal adipose stores. Equine PMN were isolated from peripheral blood with a discontinuous density gradient and stimulated with phorbol 12-myristate 13-acetate (PMA) to induce NET release as positive control. Scanning electron microscopy (SEM) and immunofluorescence microscopy (IFM) analyses were performed to assess NET release by equine PMN co-cultured with ADSCs. IFM-NET quantification revealed a significant NET decrease for PMN co-cultured with ADSCs and PMA. Furthermore, extracellular DNA quantification showed that inhibition of equine NET is dependent on the ADSCs to PMN ratio, for PMA and ionomycin stimulated PMN. Moreover, our findings unveil no modulation of reactive oxygen species (ROS) production by equine PMN when co-cultured with ADSCs. In summary, our results provide evidence of ADSCs on equine PMN, particularly in their capacity to attenuate NET formation and release. These results support the potential role of ADSCs on host innate immune response and thereby maintaining immune homeostasis. Further investigation is needed to better understand the specific molecular pathways involved in NETosis via ADSCs.
Copyright © 2025 Salinas-Varas, Espinosa, Muñoz-Caro, Conejeros, Gärtner, Fey, Arnhold, Taubert and Hermosilla.
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Publication Date: 2025-10-22 PubMed ID: 41200546PubMed Central: PMC12586003DOI: 10.3389/fvets.2025.1685757Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Overview

  • This study investigated how stem cells derived from horse fat tissue (adipose-derived stem cells or ADSCs) influence the formation of neutrophil extracellular traps (NETs) released by immune cells called polymorphonuclear neutrophils (PMNs).
  • The research found that these stem cells can reduce NET formation, suggesting a role in modulating innate immune responses and inflammation in horses.

Background

  • Neutrophil Extracellular Traps (NETs): NETs are web-like DNA structures mixed with antimicrobial proteins that PMNs release when activated, helping to trap and neutralize pathogens as part of the innate immune system.
  • Adipose-Derived Stem Cells (ADSCs): These are mesenchymal stem cells sourced from fat tissue, known for their regenerative abilities, immunomodulatory effects, and anti-inflammatory properties.
  • Despite known immune effects of ADSCs, their interaction with PMNs and impact on NET formation had been unclear before this study.

Research Objectives

  • To evaluate the effects of equine ADSCs on NET formation by equine PMNs in vitro.
  • To compare ADSCs derived from two fat tissue sources: subcutaneous and retroperitoneal adipose tissue.
  • To determine whether the presence of ADSCs affects reactive oxygen species (ROS) production by PMNs, which are involved in NET formation.

Methods

  • Isolation of Cells: ADSCs were isolated from two types of horse fat tissue; PMNs were isolated from peripheral horse blood using density gradient separation.
  • NET Induction: PMNs were stimulated using phorbol 12-myristate 13-acetate (PMA) and ionomycin to induce NET formation.
  • Co-Culture Experiments: PMNs were co-cultured with ADSCs to observe effects on NET release.
  • Visualization Techniques: Scanning electron microscopy (SEM) and immunofluorescence microscopy (IFM) were used to visualize and quantify NET release.
  • Extracellular DNA Quantification: Used as a measure of NET abundance, with analyses performed at different ADSC to PMN ratios.
  • ROS Measurement: Investigated to see if ADSCs impacted PMN oxidative burst, an important step in NETosis.

Key Findings

  • When equine PMNs were co-cultured with ADSCs and stimulated with PMA, the amount of NETs released was significantly reduced, indicating that ADSCs suppress NET formation.
  • The reduction in NET release was dependent on the ratio of ADSCs to PMNs, showing that higher numbers of ADSCs more effectively inhibited NET production.
  • This inhibitory effect was observed with both PMA- and ionomycin-stimulated PMNs.
  • No significant change was detected in the production of reactive oxygen species (ROS) by PMNs in the presence of ADSCs, suggesting ADSCs reduce NET release through mechanisms other than affecting oxidative burst.

Conclusions and Implications

  • Equine ADSCs have the ability to attenuate NET formation by PMNs, indicating they can modulate innate immune responses and potentially reduce excessive inflammation.
  • This immunomodulatory interaction highlights the therapeutic potential of ADSCs in managing immune system-related conditions in horses, especially where NETs may contribute to inflammation or tissue damage.
  • Understanding the molecular pathways by which ADSCs inhibit NETosis could reveal novel targets for controlling immune responses and improving regenerative therapies.
  • Further research is needed to dissect the exact biochemical and molecular mechanisms involved in ADSC-mediated NET regulation.

Cite This Article

APA
Salinas-Varas C, Espinosa G, Muñoz-Caro T, Conejeros I, Gärtner U, Fey K, Arnhold S, Taubert A, Hermosilla C. (2025). Equine adipose-derived stem cells modulate in vitro neutrophil extracellular trap release by polymorphonuclear neutrophils. Front Vet Sci, 12, 1685757. https://doi.org/10.3389/fvets.2025.1685757

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 12
Pages: 1685757
PII: 1685757

Researcher Affiliations

Salinas-Varas, Constanza
  • Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.
Espinosa, Gabriel
  • Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.
Muñoz-Caro, Tamara
  • Escuela de Medicina Veterinaria, Facultad de Medicina Veterinaria y Recursos Naturales, Universidad Santo Tomás, Talca, Chile.
Conejeros, Iván
  • Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.
Gärtner, Ulrich
  • Institute of Anatomy and Cell Biology, Justus Liebig University Giessen, Giessen, Germany.
Fey, Kerstin
  • Equine Clinic, Internal Medicine, Justus Liebig University Giessen, Giessen, Germany.
Arnhold, Stefan
  • Institute of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.
Taubert, Anja
  • Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.
Hermosilla, Carlos
  • Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

This article includes 56 references
  1. Ullah I, Subbarao RB, Rho GJ. Human mesenchymal stem cells - current trends and future prospective.. Biosci Rep (2015) 35:e00191.
    doi: 10.1042/bsr20150025pmc: PMC4413017pubmed: 25797907google scholar: lookup
  2. Sheng G. The developmental basis of mesenchymal stem/stromal cells (MSCs).. BMC Dev Biol (2015) 15:44.
    doi: 10.1186/s12861-015-0094-5pmc: PMC4654913pubmed: 26589542google scholar: lookup
  3. Morawska-Kozłowska M, Pitas M, Zhalniarovich Y. Mesenchymal stem cells in veterinary medicine—still untapped potential.. Animals (2025) 15:1175.
    doi: 10.3390/ani15081175pmc: PMC12024326pubmed: 40282009google scholar: lookup
  4. Ferreira-Baptista C, Ferreira R, Fernandes MH, Gomes PS, Colaço B. Influence of the anatomical site on adipose tissue-derived stromal cells’ biological profile and osteogenic potential in companion animals.. Vet Sci (2023) 10:673.
    doi: 10.3390/vetsci10120673pmc: PMC10747344pubmed: 38133224google scholar: lookup
  5. Zhang J, Liu Y, Chen Y, Yuan L, Liu H, Wang J. Adipose-derived stem cells: current applications and future directions in the regeneration of multiple tissues.. Stem Cells Int (2020) 2020:1–26.
    doi: 10.1155/2020/8810813pmc: PMC7787857pubmed: 33488736google scholar: lookup
  6. Chen B, Chen Z, He M, Zhang L, Yang L, Wei L. Recent advances in the role of mesenchymal stem cells as modulators in autoinflammatory diseases.. Front Immunol (2024) 15:1525380.
    doi: 10.3389/fimmu.2024.1525380pmc: PMC11695405pubmed: 39759531google scholar: lookup
  7. Kangari P, Talaei-Khozani T, Razeghian-Jahromi I, Razmkhah M. Mesenchymal stem cells: amazing remedies for bone and cartilage defects.. Stem Cell Res Ther (2020) 11:492.
    doi: 10.1186/s13287-020-02001-1pmc: PMC7681994pubmed: 33225992google scholar: lookup
  8. Lv Z, Cai X, Bian Y, Wei Z, Zhu W, Zhao X. Advances in mesenchymal stem cell therapy for osteoarthritis: from preclinical and clinical perspectives.. Bioengineering (2023) 10:195.
    doi: 10.3390/bioengineering10020195pmc: PMC9952673pubmed: 36829689google scholar: lookup
  9. Shi X, Chen Q, Wang F. Mesenchymal stem cells for the treatment of ulcerative colitis: a systematic review and meta-analysis of experimental and clinical studies.. Stem Cell Res Ther (2019) 10:266.
    doi: 10.1186/s13287-019-1336-4pmc: PMC6708175pubmed: 31443677google scholar: lookup
  10. Hill ABT, Bressan FF, Murphy BD, Garcia JM. Applications of mesenchymal stem cell technology in bovine species.. Stem Cell Res Ther (2019) 10:44.
    doi: 10.1186/s13287-019-1145-9pmc: PMC6345009pubmed: 30678726google scholar: lookup
  11. Beerts C, Suls M, Broeckx SY, Seys B, Vandenberghe A, Declercq J. Tenogenically induced allogeneic peripheral blood mesenchymal stem cells in allogeneic platelet-rich plasma: 2-year follow-up after tendon or ligament treatment in horses.. Front Vet Sci (2017) 4:158.
    doi: 10.3389/fvets.2017.00158pmc: PMC5622984pubmed: 29018808google scholar: lookup
  12. Carlier S, Depuydt E, Suls M, Bocqué C, Thys J, Vandenberghe A. Equine allogeneic tenogenic primed mesenchymal stem cells: a clinical field study in horses suffering from naturally occurring superficial digital flexor tendon and suspensory ligament injuries.. Equine Vet J (2024) 56:924–35.
    doi: 10.1111/evj.14008pubmed: 37847100google scholar: lookup
  13. Leal Reis I, Lopes B, Sousa P, Sousa AC, Branquinho M, Caseiro AR. Allogenic synovia-derived mesenchymal stem cells for treatment of equine tendinopathies and Desmopathies—proof of concept.. Animals (2023) 13:1312.
    doi: 10.3390/ani13081312pmc: PMC10135243pubmed: 37106875google scholar: lookup
  14. Carvalho AM, Yamada ALM, Golim MA, Álvarez LEC, Jorge LL, Conceição ML. Characterization of mesenchymal stem cells derived from equine adipose tissue.. Arq Bras Med Vet Zootec (2013) 65:939–45.
    doi: 10.1590/s0102-09352013000400001google scholar: lookup
  15. Esteves CL, Sheldrake TA, Mesquita SP, Pesántez JJ, Menghini T, Dawson L. Isolation and characterization of equine native MSC populations.. Stem Cell Res Ther (2017) 8:80.
    doi: 10.1186/s13287-017-0525-2pmc: PMC5395828pubmed: 28420427google scholar: lookup
  16. McDonald B, Urrutia R, Yipp BG, Jenne CN, Kubes P. Intravascular neutrophil extracellular traps capture Bacteria from the bloodstream during Sepsis. Cell Host Microbe (2012) 12:324–33.
    doi: 10.1016/j.chom.2012.06.011pubmed: 22980329google scholar: lookup
  17. Song N, Scholtemeijer M, Shah K. Mesenchymal stem cell immunomodulation: mechanisms and therapeutic potential. Trends Pharmacol Sci (2020) 41:653–64.
    doi: 10.1016/j.tips.2020.06.009pmc: PMC7751844pubmed: 32709406google scholar: lookup
  18. Yuan Z, Jiang D, Yang M, Tao J, Hu X, Yang X. Emerging roles of macrophage polarization in osteoarthritis: mechanisms and therapeutic strategies. Orthop Surg (2024) 16:532–50.
    doi: 10.1111/os.13993pmc: PMC10925521pubmed: 38296798google scholar: lookup
  19. Le Blanc K, Davies LC. Mesenchymal stromal cells and the innate immune response. Immunol Lett (2015) 168:140–6.
    doi: 10.1016/j.imlet.2015.05.004pubmed: 25982165google scholar: lookup
  20. De Oliveira S, Rosowski EE, Huttenlocher A. Neutrophil migration in infection and wound repair: going forward in reverse. Nat Rev Immunol (2016) 16:378–91.
    doi: 10.1038/nri.2016.49pmc: PMC5367630pubmed: 27231052google scholar: lookup
  21. Burn GL, Foti A, Marsman G, Patel DF, Zychlinsky A. The neutrophil. Immunity (2021) 54:1377–91.
    doi: 10.1016/j.immuni.2021.06.006pubmed: 34260886google scholar: lookup
  22. Papayannopoulos V. Neutrophil extracellular traps in immunity and disease. Nat Rev Immunol (2018) 18:134–47.
    doi: 10.1038/nri.2017.105pubmed: 28990587google scholar: lookup
  23. Wang H, Kim SJ, Lei Y, Wang S, Wang H, Huang H. Neutrophil extracellular traps in homeostasis and disease. Signal Transduct Target Ther (2024) 9:235.
    doi: 10.1038/s41392-024-01933-xpmc: PMC11415080pubmed: 39300084google scholar: lookup
  24. Wigerblad G, Kaplan MJ. Neutrophil extracellular traps in systemic autoimmune and autoinflammatory diseases. Nat Rev Immunol (2023) 23:274–88.
    doi: 10.1038/s41577-022-00787-0pmc: PMC9579530pubmed: 36257987google scholar: lookup
  25. Zaripova LN, Midgley A, Christmas SE, Beresford MW, Pain C, Baildam EM. Mesenchymal stem cells in the pathogenesis and therapy of autoimmune and autoinflammatory diseases. Int J Mol Sci (2023) 24:16040.
    doi: 10.3390/ijms242216040pmc: PMC10671211pubmed: 38003230google scholar: lookup
  26. Zhao Q, Ren H, Han Z. Mesenchymal stem cells: immunomodulatory capability and clinical potential in immune diseases. J Cell Immunother (2016) 2:3–20.
    doi: 10.1016/j.jocit.2014.12.001google scholar: lookup
  27. Feng B, Feng X, Yu Y, Xu H, Ye Q, Hu R. Mesenchymal stem cells shift the pro-inflammatory phenotype of neutrophils to ameliorate acute lung injury. Stem Cell Res Ther (2023) 14:197.
    doi: 10.1186/s13287-023-03438-wpmc: PMC10408228pubmed: 37553691google scholar: lookup
  28. Joel MDM, Yuan J, Wang J, Yan Y, Qian H, Zhang X. MSC: immunoregulatory effects, roles on neutrophils and evolving clinical potentials. Am J Transl Res (2019) 11:3890–904.
    pmc: PMC6614638pubmed: 31312397
  29. Taghavi-Farahabadi M, Mahmoudi M, Rezaei N, Hashemi SM. Wharton’s jelly mesenchymal stem cells exosomes and conditioned media increased neutrophil lifespan and phagocytosis capacity. Immunol Investig (2021) 50:1042–57.
    doi: 10.1080/08820139.2020.1801720pubmed: 32777963google scholar: lookup
  30. Mahmoudi M, Taghavi-Farahabadi M, Namaki S, Baghaei K, Rayzan E, Rezaei N. Exosomes derived from mesenchymal stem cells improved function and survival of neutrophils from severe congenital neutropenia patients in vitro. Hum Immunol (2019) 80:990–8.
    doi: 10.1016/j.humimm.2019.10.006pubmed: 31706743google scholar: lookup
  31. Jiang D, Muschhammer J, Qi Y, Kügler A, de Vries JC, Saffarzadeh M. Suppression of neutrophil-mediated tissue damage—a novel skill of mesenchymal stem cells. Stem Cells (2016) 34:2393–406.
    doi: 10.1002/stem.2417pmc: PMC5572139pubmed: 27299700google scholar: lookup
  32. Xu K, Cooney KA, Shin EY, Wang L, Deppen JN, Ginn SC. Adenosine from a biologic source regulates neutrophil extracellular traps (NETs). J Leukoc Biol (2019) 105:1225–34.
    doi: 10.1002/jlb.3vma0918-374rpmc: PMC6536338pubmed: 30907983google scholar: lookup
  33. Salami F, Ghodrati M, Parham A, Mehrzad J. The effect of equine bone marrow-derived mesenchymal stem cells on the expression of apoptotic genes in neutrophils. Vet Med Sci (2021) 7:626–33.
    doi: 10.1002/vms3.427pmc: PMC8136922pubmed: 33471967google scholar: lookup
  34. Espinosa G, Plaza A, Schenffeldt A, Alarcón P, Gajardo G, Uberti B. Equine bone marrow-derived mesenchymal stromal cells inhibit reactive oxygen species production by neutrophils. Vet Immunol Immunopathol (2020) 221:109975.
    doi: 10.1016/j.vetimm.2019.109975pubmed: 32087476google scholar: lookup
  35. Siemsen DW, Malachowa N, Schepetkin IA, Whitney AR, Kirpotina LN, Lei B. Neutrophil isolation from nonhuman species. Methods Mol Biol (2014) 1124:19–37.
    doi: 10.1007/978-1-62703-845-4_3pmc: PMC6777848pubmed: 24504944google scholar: lookup
  36. Raabe O, Reich C, Wenisch S, Hild A, Burg-Roderfeld M, Siebert H-C. Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells. Histochem Cell Biol (2010) 134:545–54.
    doi: 10.1007/s00418-010-0760-4pubmed: 21076963google scholar: lookup
  37. Rodrigues M, Turner O, Stolz D, Griffith LG, Wells A. Production of reactive oxygen species by multipotent stromal cells/mesenchymal stem cells upon exposure to Fas ligand. Cell Transplant (2012) 21:2171–87.
    doi: 10.3727/096368912X639035pmc: PMC3876879pubmed: 22526333google scholar: lookup
  38. Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss DS. Neutrophil extracellular traps kill Bacteria. Science (2004) 303:1532–5.
    doi: 10.1126/science.1092385pubmed: 15001782google scholar: lookup
  39. Franck T, Ceusters J, Graide H, Mouithys-Mickalad A, Serteyn D. Muscle derived mesenchymal stem cells inhibit the activity of the free and the neutrophil extracellular trap (NET)-bond myeloperoxidase. Cells (2021) 10:3486.
    doi: 10.3390/cells10123486pmc: PMC8700239pubmed: 34943996google scholar: lookup
  40. Morandini L, Avery D, Angeles B, Winston P, Martin RK, Donahue HJ. Reduction of neutrophil extracellular traps accelerates inflammatory resolution and increases bone formation on titanium implants. Acta Biomater (2023) 166:670–84.
    doi: 10.1016/j.actbio.2023.05.016pmc: PMC10330750pubmed: 37187302google scholar: lookup
  41. Azzouz D, Palaniyar N. How do ROS induce NETosis? Oxidative DNA damage, DNA repair, and chromatin Decondensation. Biomolecules (2024) 14:1307.
    doi: 10.3390/biom14101307pmc: PMC11505619pubmed: 39456240google scholar: lookup
  42. Salami F, Tavassoli A, Mehrzad J, Parham A. Immunomodulatory effects of mesenchymal stem cells on leukocytes with emphasis on neutrophils. Immunobiology (2018) 223:786–91.
    doi: 10.1016/j.imbio.2018.08.002pubmed: 30119931google scholar: lookup
  43. Magaña-Guerrero FS, Domínguez-López A, Martínez-Aboytes P, Buentello-Volante B, Garfias Y. Human amniotic membrane mesenchymal stem cells inhibit neutrophil extracellular traps through TSG-6. Sci Rep (2017) 7:12426.
    doi: 10.1038/s41598-017-10962-2pmc: PMC5622031pubmed: 28963485google scholar: lookup
  44. Wang LT, Lee W, Liu KJ, Sytwu HK, Yen ML, Yen BL. Mesenchymal stromal/stem cells know best: the remarkable complexities of its interactions with Polymorphonuclear neutrophils. Stem Cells (2024) 42:403–15.
    doi: 10.1093/stmcls/sxae011pubmed: 38310524google scholar: lookup
  45. Mumaw JL, Schmiedt CW, Breidling S, Sigmund A, Norton NA, Thoreson M. Feline mesenchymal stem cells and supernatant inhibit reactive oxygen species production in cultured feline neutrophils. Res Vet Sci (2015) 103:60–9.
    doi: 10.1016/j.rvsc.2015.09.010pubmed: 26679797google scholar: lookup
  46. Müller L, Tunger A, Wobus M, Von Bonin M, Towers R, Bornhäuser M. Immunomodulatory properties of mesenchymal stromal cells: an update. Front Cell Dev Biol (2021) 9:637725.
    doi: 10.3389/fcell.2021.637725pmc: PMC7900158pubmed: 33634139google scholar: lookup
  47. Pruchniak MP, Demkow U. Potent NETosis inducers do not show synergistic effects in vitro. Cent Eur J Immunol (2019) 44:51–8.
    doi: 10.5114/ceji.2019.84017pmc: PMC6526581pubmed: 31114437google scholar: lookup
  48. Franck T, Ceusters J, Graide H, Niesten A, Duysens J, Mickalad AM. Equine muscle derived mesenchymal stem cells loaded with water-soluble curcumin: modulation of neutrophil activation and enhanced protection against intracellular oxidative attack. Int J Mol Sci (2023) 24:1030.
    doi: 10.3390/ijms24021030pmc: PMC9865820pubmed: 36674546google scholar: lookup
  49. Conejeros I, Velásquez ZD, Grob D, Zhou E, Salecker H, Hermosilla C. Histone H2A and bovine neutrophil extracellular traps induce damage of Besnoitia besnoiti-infected host endothelial cells but fail to affect Total parasite proliferation. Biology (2019) 8:78.
    doi: 10.3390/biology8040078pmc: PMC6956067pubmed: 31614617google scholar: lookup
  50. Islam MM, Takeyama N. Role of neutrophil extracellular traps in health and disease pathophysiology: recent insights and advances. Int J Mol Sci (2023) 24:15805.
    doi: 10.3390/ijms242115805pmc: PMC10649138pubmed: 37958788google scholar: lookup
  51. Retter A, Singer M, Annane D. “The NET effect”: neutrophil extracellular traps—a potential key component of the dysregulated host immune response in sepsis. Crit Care (2025) 29:59.
    doi: 10.1186/s13054-025-05283-0pmc: PMC11796136pubmed: 39905519google scholar: lookup
  52. Birckhead EM, Das S, Tidd N, Raidal SL, Raidal SR. Visualizing neutrophil extracellular traps in septic equine synovial and peritoneal fluid samples using immunofluorescence microscopy. J Vet Diagn Invest (2023) 35:751–60.
    doi: 10.1177/10406387231196552pmc: PMC10621558pubmed: 37661696google scholar: lookup
  53. Janssen P, Tosi I, Hego A, Maréchal P, Marichal T, Radermecker C. Neutrophil extracellular traps are found in Bronchoalveolar lavage fluids of horses with severe asthma and correlate with asthma severity. Front Immunol (2022) 13:921077.
    doi: 10.3389/fimmu.2022.921077pmc: PMC9326094pubmed: 35911691google scholar: lookup
  54. Storms N, De La Rebière G, Franck T, Mouithys Mickalad A, Sandersen C, Ceusters J. Neutrophil extracellular traps and active myeloperoxidase concentrate in lamellar tissue of equids with naturally occurring laminitis. Vet Immunol Immunopathol (2024) 270:110738.
    doi: 10.1016/j.vetimm.2024.110738pubmed: 38452577google scholar: lookup
  55. Rohrbach AS, Slade DJ, Thompson PR, Mowen KA. Activation of PAD4 in NET formation. Front Immunol (2012) 3:360.
    doi: 10.3389/fimmu.2012.00360pmc: PMC3525017pubmed: 23264775google scholar: lookup
  56. Arnhold S, Elashry MI, Klymiuk MC, Geburek F. Investigation of stemness and multipotency of equine adipose-derived mesenchymal stem cells (ASCs) from different fat sources in comparison with lipoma. Stem Cell Res Ther (2019) 10:309.
    doi: 10.1186/s13287-019-1429-0pmc: PMC6805636pubmed: 31640774google scholar: lookup

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