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Equine veterinary journal2014; 47(3); 319-325; doi: 10.1111/evj.12288

Equine autologous platelet concentrates: A comparative study between different available systems.

Abstract: Autologous platelet concentrates (APCs) are being used increasingly in horses to enhance regeneration in tissues that have poor natural healing capabilities. Numerous APC systems, which are based on different preparation techniques and were originally developed for human patients, are now routinely used in equine cases. However, preliminary process validation and adequate in vitro biochemical characterisation of most of these systems do not exist for horses. Objective: To compare haematological findings and growth factor concentrations of equine APCs obtained with 4 commercially available systems and a noncommercial double-centrifugation technique. Methods: Nonrandomised in vitro experiment. Methods: Blood samples from 6 horses were processed to produce APCs using one equine-specific filtration-based and 4 different centrifugation-based techniques. Platelet, leucocyte, platelet-derived growth factor-BB and transforming growth factor-β1 concentrations were measured in all APCs, and their respective enrichment factors were compared. Results: Mean platelet concentration increased in all systems in comparison to baseline; however, the mean enrichment factor, which ranged from 130% to 527% depending on the APC, was statistically significant in only 2 products. One method reduced total leucocyte counts to 9% of the baseline value, while the others had a mean fold increase varying from 116 to 663% of the baseline. Differential leucocyte count also differed between the products. Moreover, the various systems had significantly different mean growth factor enrichments (184-1255% for platelet-derived growth factor-BB and 93-560% for transforming growth factor-β1 ). Conclusions: Haematological and biochemical characteristics varied markedly among 5 techniques used in the field to produce APCs in horses. These discrepancies could have an impact on clinical outcomes, and further studies are needed to determine their influence on the quality of tissue regeneration. Clinicians should not rely on the manufacturers' data relating to human patients to select the most appropriate method for horses.
© 2014 EVJ Ltd.
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Publication Date: 2014-06-11 PubMed ID: 24773596DOI: 10.1111/evj.12288Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research compares different techniques for producing horse platelet concentrates, a treatment method used to help tissue regeneration. The results demonstrate that these techniques vary in their effectiveness, which could impact treatment outcomes.

Introduction

  • The study examined autologous platelet concentrates (APCs), which are derived from a horse’s own blood and help to increase tissue regeneration in areas that naturally struggle to heal.
  • While APCs were initially created for human patients, they are now being increasingly used in the treatment of horses.
  • Despite their broad adoption, there is little process validation or biochemical characterisation for most APC systems in horses.

Aim of the Study

  • The research aimed to compare the haematological findings and growth factor concentrations in horse APCs using five different systems.
  • The systems included one specific to horses and based on filtration and four that use centrifugation.

Methodology

  • The nonrandomised in vitro experiment involved extracting and processing blood samples from six different horses.
  • The researchers then compared platelet, leucocyte, platelet-derived growth factor-BB, and transforming growth factor-β1 concentrations in the resulting APCs.
  • The respective enrichment factors of each of these components were also analysed and compared.

Results

  • The results showed that the platelet concentration increased in all systems compared to baseline, but the enrichment factor, ranged from 130% to 527%, was only statistically significant in two products.
  • Some systems significantly increased the leucocyte count, up to 663% of the baseline, while one decreased it to just 9%.
  • There were also discrepancies in the differential leucocyte count among the different systems.
  • The mean growth factor enrichments also differed significantly among the systems (184-1255% for platelet-derived growth factor-BB and 93-560% for transforming growth factor-β1).

Conclusions

  • The research concluded that the different techniques used to create equine APCs varied substantially, which could potentially affect clinical outcomes.
  • Further studies are needed to determine the effect of these discrepancies on tissue regeneration.
  • The study highlights the importance of not relying on manufacturers’ data related to human patients when selecting an appropriate method for equine patients.

Cite This Article

APA
Hessel LN, Bosch G, van Weeren PR, Ionita JC. (2014). Equine autologous platelet concentrates: A comparative study between different available systems. Equine Vet J, 47(3), 319-325. https://doi.org/10.1111/evj.12288

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 47
Issue: 3
Pages: 319-325

Researcher Affiliations

Hessel, L N
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Bosch, G
    van Weeren, P R
      Ionita, J-C

        MeSH Terms

        • Animals
        • Cell Separation / methods
        • Cell Separation / veterinary
        • Female
        • Horses / blood
        • Intercellular Signaling Peptides and Proteins / chemistry
        • Male
        • Platelet-Rich Plasma / chemistry
        • Platelet-Rich Plasma / cytology
        • Transplantation, Autologous

        Citations

        This article has been cited 24 times.
        1. Mayet A, Zablotski Y, Roth SP, Brehm W, Troillet A. Systematic review and meta-analysis of positive long-term effects after intra-articular administration of orthobiologic therapeutics in horses with naturally occurring osteoarthritis. Front Vet Sci 2023;10:1125695.
          doi: 10.3389/fvets.2023.1125695pubmed: 36908512google scholar: lookup
        2. Maleas G, Mageed M. Effectiveness of Platelet-Rich Plasma and Bone Marrow Aspirate Concentrate as Treatments for Chronic Hindlimb Proximal Suspensory Desmopathy. Front Vet Sci 2021;8:678453.
          doi: 10.3389/fvets.2021.678453pubmed: 34222402google scholar: lookup
        3. Seidel SRT, de Souza AF, Fülber J, Bogossian PM, Rodrigues NNP, Baccarin RYA. Evaluation of platelet-rich plasma applied in the coronary band of healthy equine hooves. Can Vet J 2021 Jul;62(7):729-735.
          pubmed: 34219782
        4. Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals (Basel) 2021 May 21;11(6).
          doi: 10.3390/ani11061478pubmed: 34063777google scholar: lookup
        5. Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade. Front Vet Sci 2021;8:641818.
          doi: 10.3389/fvets.2021.641818pubmed: 33869321google scholar: lookup
        6. King W, Cawood K, Bookmiller M. The Use of Autologous Protein Solution (Pro-Stride(®)) and Leukocyte-Rich Platelet-Rich Plasma (Restigen(®)) in Canine Medicine. Vet Med (Auckl) 2021;12:53-65.
          doi: 10.2147/VMRR.S286913pubmed: 33777723google scholar: lookup
        7. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
          doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
        8. Gonçalves NJN, Frantz N, de Oliveira RM. Platelet-rich plasma (PRP) therapy: An approach in reproductive medicine based on successful animal models. Anim Reprod 2020 May 22;16(1):93-98.
          doi: 10.21451/1984-3143-AR2018-093pubmed: 33299482google scholar: lookup
        9. Perego R, Spada E, Baggiani L, Martino PA, Proverbio D. Efficacy of a Semi Automated Commercial Closed System for Autologous Leukocyte- and Platelet-Rich Plasma (l-prp) Production in Dogs: A Preliminary Study. Animals (Basel) 2020 Aug 4;10(8).
          doi: 10.3390/ani10081342pubmed: 32759643google scholar: lookup
        10. Du X, Liu X, Mawolo JB, Wang H, Mi X, Dong J, Li Q, Wen Y. Determination of haematological and biochemical parameters of Calf Saiga antelope (Saiga tatarica) living in the Gansu Endangered Animals Research Center. Vet Med Sci 2020 Aug;6(3):591-599.
          doi: 10.1002/vms3.236pubmed: 31981463google scholar: lookup
        11. Seidel SRT, Vendruscolo CP, Moreira JJ, Fülber J, Ottaiano TF, Oliva MLV, Michelacci YM, Baccarin RYA. Does Double Centrifugation Lead to Premature Platelet Aggregation and Decreased TGF-β1 Concentrations in Equine Platelet-Rich Plasma?. Vet Sci 2019 Aug 21;6(3).
          doi: 10.3390/vetsci6030068pubmed: 31438534google scholar: lookup
        12. McClain AK, McCarrel TM. The effect of four different freezing conditions and time in frozen storage on the concentration of commonly measured growth factors and enzymes in equine platelet-rich plasma over six months. BMC Vet Res 2019 Aug 14;15(1):292.
          doi: 10.1186/s12917-019-2040-4pubmed: 31412868google scholar: lookup
        13. Lee EB, Kim JW, Seo JP. Comparison of the methods for platelet rich plasma preparation in horses. J Anim Sci Technol 2018;60:20.
          doi: 10.1186/s40781-018-0178-4pubmed: 30147942google scholar: lookup
        14. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know. Front Vet Sci 2018;5:70.
          doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
        15. Gutiérrez CM, López C, Giraldo CE, Carmona JU. Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma. Vet Med Int 2017;2017:1950401.
          doi: 10.1155/2017/1950401pubmed: 29214094google scholar: lookup
        16. Franklin SP, Birdwhistell KE, Strelchik A, Garner BC, Brainard BM. Influence of Cellular Composition and Exogenous Activation on Growth Factor and Cytokine Concentrations in Canine Platelet-Rich Plasmas. Front Vet Sci 2017;4:40.
          doi: 10.3389/fvets.2017.00040pubmed: 28424777google scholar: lookup
        17. Hauschild G, Geburek F, Gosheger G, Eveslage M, Serrano D, Streitbürger A, Johannlükens S, Menzel D, Mischke R. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations. BMC Vet Res 2017 Jan 5;13(1):7.
          doi: 10.1186/s12917-016-0920-4pubmed: 28056978google scholar: lookup
        18. Bembo F, Eraud J, Philandrianos C, Bertrand B, Silvestre A, Veran J, Sabatier F, Magalon G, Magalon J. Combined use of platelet rich plasma & micro-fat in sport and race horses with degenerative joint disease: preliminary clinical study in eight horses. Muscles Ligaments Tendons J 2016 Apr-Jun;6(2):198-204.
          doi: 10.11138/mltj/2016.6.2.198pubmed: 27900293google scholar: lookup
        19. Geburek F, Gaus M, van Schie HT, Rohn K, Stadler PM. Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies - a randomized prospective controlled clinical trial. BMC Vet Res 2016 Sep 7;12(1):191.
          doi: 10.1186/s12917-016-0826-1pubmed: 27604193google scholar: lookup
        20. Mirza MH, Bommala P, Richbourg HA, Rademacher N, Kearney MT, Lopez MJ. Gait Changes Vary among Horses with Naturally Occurring Osteoarthritis Following Intra-articular Administration of Autologous Platelet-Rich Plasma. Front Vet Sci 2016;3:29.
          doi: 10.3389/fvets.2016.00029pubmed: 27148544google scholar: lookup
        21. Gomiero C, Bertolutti G, Martinello T, Van Bruaene N, Broeckx SY, Patruno M, Spaas JH. Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology. Vet Res Commun 2016 Mar;40(1):39-48.
          doi: 10.1007/s11259-016-9652-ypubmed: 26757735google scholar: lookup
        22. Carmona JU, López C, Argüelles D. Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation. Animals (Basel) 2025 Dec 13;15(24).
          doi: 10.3390/ani15243586pubmed: 41463871google scholar: lookup
        23. Guerra-Gomes M, Ferreira-Baptista C, Barros J, Alves-Pimenta S, Gomes P, Colaço B. Exploring the Potential of Non-Cellular Orthobiologic Products in Regenerative Therapies for Stifle Joint Diseases in Companion Animals. Animals (Basel) 2025 Feb 18;15(4).
          doi: 10.3390/ani15040589pubmed: 40003071google scholar: lookup
        24. Bernardini C, Romagnoli N, Casalini I, Turba ME, Spadari A, Forni M, Gentilini F. Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate. Int J Vet Sci Med 2024;12(1):71-80.
          doi: 10.1080/23144599.2024.2380586pubmed: 39119550google scholar: lookup
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