Equine colostral carbohydrates reduce lipopolysaccharide-induced inflammatory responses in equine peripheral blood mononuclear cells.
Abstract: Increasing evidence suggests that reactions to lipopolysaccharide (LPS), particularly in the gut, can be partly or completely mitigated by colostrum- and milk-derived oligosaccharides. Confirmation of this hypothesis could lead to the development of new therapeutic concepts. Objective: To demonstrate the influence of equine colostral carbohydrates on the inflammatory response in an in vitro model with equine peripheral blood mononuclear cells (PBMCs). Methods: Carbohydrates were extracted from mare colostrum, and then evaluated for their influence on LPS-induced inflammatory responses in PBMCs isolated from the same mares, mRNA expression of tumour necrosis factor-alpha, interleukin-6 and interleukin-10 was measured as well as the protein levels of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10). Results: Equine colostral carbohydrates significantly reduced LPS-induced TNF-alpha protein at both times measured and significantly reduced LPS-induced TNF-alpha, IL-6 and IL-10 mRNA expression by PBMCs. Moreover, cell viability significantly increased in the presence of high concentrations of colostral carbohydrates. Conclusions: Carbohydrates derived from equine colostrum reduce LPS-induced inflammatory responses of equine PBMCs. Conclusions: Colostrum and milk-derived carbohydrates are promising candidates for new concepts in preventive and regenerative medicine.
Publication Date: 2013-03-02 PubMed ID: 23447881DOI: 10.1111/j.2042-3306.2012.00680.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates how carbohydrates derived from horse colostrum can reduce inflammatory responses related to lipopolysaccharide in horse peripheral blood mononuclear cells. The findings indicate that these carbohydrates significantly lessen the release of certain inflammatory proteins and boost cell viability.
Research Objective
- This research looks into the role of equine colostral carbohydrates in counteracting the inflammatory response caused by lipopolysaccharide (a component of the outer membrane of certain bacteria) in an in-vitro model revolving around equine peripheral blood mononuclear cells (PBMCs).
Methods Used
- The team extracted carbohydrates from mare colostrum, which is the nutrient-rich pre-milk fluid produced by mammalian mothers in the initial days after birth.
- The researchers then evaluated these carbohydrates’ impact on LPS-induced (Lipopolysaccharide-induced) inflammatory responses in PBMCs isolated from the same mares from where the colostrum was taken.
- The expression of certain proteins (tumor necrosis factor-alpha, interleukin-6, and interleukin-10) at both the mRNA level and protein level were measured. Such proteins are known to be pivotal in regulating the immune response and, in particularly, triggering inflammation.
Research Findings
- Equine colostral carbohydrates extensively lowered the production of the inflammatory protein TNF-alpha and mRNA expression of TNF-alpha, IL-6, and IL-10 induced by LPS.
- Furthermore, an increased cell viability was recorded in scenarios involving high concentrations of colostral carbohydrates. Cell viability is a measure of the proportion of existing living cells in a population and is an important determinant of the health and functionality of cells.
Overall Conclusion
- The experiment demonstrates that carbohydrates drawn from equine colostrum possess the capability to considerably decrease the inflammatory responses in equine PBMCs triggered by LPS.
- This leads to the conclusion that colostrum and milk-derived carbohydrates could become promising tools in new approaches towards preventive and regenerative medicine.
Cite This Article
APA
Vendrig JC, Coffeng LE, Fink-Gremmels J.
(2013).
Equine colostral carbohydrates reduce lipopolysaccharide-induced inflammatory responses in equine peripheral blood mononuclear cells.
Equine Vet J Suppl(43), 68-72.
https://doi.org/10.1111/j.2042-3306.2012.00680.x Publication
Researcher Affiliations
- Faculty of Veterinary Medicine, Veterinary Pharmacology, Pharmacotherapy and Toxicology, Utrecht University, The Netherlands. j.c.vendrig@uu.nl
MeSH Terms
- Animals
- Carbohydrates / analysis
- Carbohydrates / pharmacology
- Cell Survival / drug effects
- Cells, Cultured
- Colostrum / chemistry
- Female
- Horses / blood
- Leukocytes, Mononuclear / drug effects
- Leukocytes, Mononuclear / metabolism
- Lipopolysaccharides / toxicity
- RNA, Messenger / genetics
- RNA, Messenger / metabolism
Citations
This article has been cited 2 times.- Gugliandolo E, Crupi R, Biondi V, Licata P, Cuzzocrea S, Passantino A. Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study.. Animals (Basel) 2020 Nov 3;10(11).
- Vendrig JC, Coffeng LE, Fink-Gremmels J. Effects of orally administered galacto-oligosaccharides on immunological parameters in foals: a pilot study.. BMC Vet Res 2014 Nov 19;10:278.
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