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Home / Equine Research Bank / J Virol / Equine connective tissue tumors contain unintegrated bovine ...
Journal of virology1980; 35(3); 962-964; doi: 10.1128/JVI.35.3.962-964.1980

Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA.

Abstract: Bovine papilloma virus (BPV) appears to be the etiological agent of common equine connective tissue tumors. We investigated the physical state of the viral DNA within such tumors and found no indication for integration into the host genome. The BPV genomes were present as free circular episomes. Two equine sarcoids were shown to contain multiple copies of free circular BPV type 1 (BPV-1) DNA. When the tumors were digested with several single-cut restriction enzymes, there were only form III BPV-1 DNA sequences could be revealed. One of the sarcoids contained, apart from wild-type BPV-1 DNA, a class of smaller BPV-1 circular DNA molecules bearing a deletion of approximately 9% of the BPV-1 genome. This deletion is located in the physical map between the relative units 0 and 0.32.
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Publication Date: 1980-09-01 PubMed ID: 6252350PubMed Central: PMC288890DOI: 10.1128/JVI.35.3.962-964.1980Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the investigation of equine connective tissue tumors and the presence of bovine papillomavirus (BPV) DNA within the tumors. Findings suggest that the BPV DNA doesn’t integrate with the host genome and exists as independent, circular entities within the tumor.

Introduction

  • The study revolves around bovine papillomavirus (BPV), which is believed to be a causative agent of common equine connective tissue tumors.
  • The primary goal of the research was to investigate the physical state of the viral DNA within such tumors.

Observations

  • No evidence was found that indicated the integration of BPV DNA into the host genome—the genetic material of the horse’s cells.
  • The viral genomes existed as circular units, or episomes—independent of the host DNA.

BPV-1 in Equine Sarcoids

  • Two equine sarcoids (skin tumors in horses) contained numerous copies of free, circular BPV-1 DNA. BPV-1 refers to type 1 of the Bovine Papillomavirus.
  • On subjecting the tumors to various single-cut restriction enzymes (used to cut DNA at specific locations), only the form III BPV-1 DNA sequences were revealed—confirming the presence of non-integrated, free viral DNA.

Deletion in BPV-1 genome

  • In one of the sarcoids, the researchers found not just wild-type BPV-1 DNA (the typical form found in nature), but also a smaller class of BPV-1 circular DNA molecules that had approximately 9% of the BPV-1 genome missing.
  • This deletion was located between the relative units 0 and 0.32 on the genome mapping.

Conclusion

  • The research concludes that the BPV DNA present in equine tumors are not integrated into the host DNA, but instead, exist independently within the tumor cells.
  • This discovery could provide new insights into the etiology and development of these types of tumors.

Cite This Article

APA
Amtmann E, Müller H, Sauer G. (1980). Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA. J Virol, 35(3), 962-964. https://doi.org/10.1128/JVI.35.3.962-964.1980

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 35
Issue: 3
Pages: 962-964

Researcher Affiliations

Amtmann, E
    Müller, H
      Sauer, G

        MeSH Terms

        • Animals
        • Bovine papillomavirus 1 / genetics
        • DNA, Viral / genetics
        • Genes, Viral
        • Horse Diseases / microbiology
        • Horses
        • Neoplasms, Connective Tissue / microbiology
        • Neoplasms, Connective Tissue / veterinary
        • Papillomaviridae / genetics
        • Plasmids
        • Recombination, Genetic
        • Tumor Virus Infections / microbiology
        • Tumor Virus Infections / veterinary

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