Equine cryptosporidial infection associated with Cryptosporidium hedgehog genotype in Algeria.
Abstract: Faecal samples from two horse farms in Algeria keeping Arabian, Thoroughbred, and Barb horses were examined for the presence of Cryptosporidium in 2010-2011. A total of 138 faecal samples (16 from a farm keeping 50 animals and 122 from a farm with 267 horses) were screened for Cryptosporidium spp. infection using molecular tools. DNA was extracted from all samples. Nested PCR was performed to amplify fragments of the SSU rDNA and gp60 genes to determine the presence of Cryptosporidium species and genotypes. Sequence analyses of SSU and gp60 genes revealed four animals positive for the presence of subtype XIIIa A22R9 of the Cryptosporidium hedgehog genotype. The infections were not associated with diarrhoea. This study reports, for the first time, the occurrence of Cryptosporidium in Algeria and the first occurrence of the hedgehog genotype in horses. These findings support the potential role of infected horses in sylvatic-domestic transmission of Cryptosporidium.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication Date: 2013-05-09 PubMed ID: 23731858DOI: 10.1016/j.vetpar.2013.04.041Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article is about the first identification of Cryptosporidium, specifically the hedgehog genotype, in horse fecal samples from two farms in Algeria.
Research Objective and Methodology
- The researchers aimed to identify cases of Cryptosporidium infection among horses in Algeria, with various breeds of horses under study including Arabian, Thoroughbred, and Barb.
- They collected and analysed a total of 138 fecal samples between 2010 and 2011: 16 from a farm with 50 a market at 122 from another farm with 267 horses.
- The detection of Cryptosporidium infection was achieved using advanced molecular techniques, mainly the extraction of DNA from the samples and a successive nested Polymerase Chain Reaction (PCR).
- The PCR was used to amplify fragments of the SSU rDNA and gp60 genes, key markers to ascertain the presence and type of Cryptosporidium species.
Findings
- Analysis of the SSU and gp60 genes showed that four animals were positive for the subtype XIIIa A22R9 of the Cryptosporidium hedgehog genotype. This was an important identification as this subtype has never been identified in horses before.
- The researchers noted that the detected infection was not associated with diarrhoea, a common symptom of Cryptosporidium infection in many hosts.
- This study marks the first reported occurrence of Cryptosporidium infection in Algerian horses and the first recorded occurrence of the hedgehog subtype in horses.
Conclusion
- The findings provide new insights into the potential role of horses in the sylvatic-domestic transmission cycle of Cryptosporidium. This means that horses could possibly act as a transmission channel for Cryptosporidium from wildlife reservoirs to domesticated animals.
Cite This Article
APA
Laatamna AE, Wagnerová P, Sak B, Květoňová D, Aissi M, Rost M, Kváč M.
(2013).
Equine cryptosporidial infection associated with Cryptosporidium hedgehog genotype in Algeria.
Vet Parasitol, 197(1-2), 350-353.
https://doi.org/10.1016/j.vetpar.2013.04.041 Publication
Researcher Affiliations
- Higher National School of Veterinary, BP 161 Hacène Badi, EL Harrach, Algiers, Algeria.
MeSH Terms
- Algeria / epidemiology
- Animals
- Cryptosporidiosis / epidemiology
- Cryptosporidiosis / parasitology
- Cryptosporidiosis / veterinary
- Cryptosporidium / genetics
- Feces / parasitology
- Hedgehogs / parasitology
- Horse Diseases / epidemiology
- Horse Diseases / parasitology
- Horses
- Lansoprazole
- Oocysts
- Phylogeny
Citations
This article has been cited 13 times.- Li XM, Geng HL, Wei YJ, Yan WL, Liu J, Wei XY, Zhang M, Wang XY, Zhang XX, Liu G. Global prevalence and risk factors of Cryptosporidium infection in Equus: A systematic review and meta-analysis.. Front Cell Infect Microbiol 2022;12:1072385.
- Reghaissia N, Maxamhud S, Laatamna A, Samari H, Dahmane A, Berima R, Abdelli A, Hakem A, Baroudi D, Tsaousis AD. First Epidemiological Report on the Prevalence and Associated Risk Factors of Cryptosporidium spp. in Farmed Marine and Wild Freshwater Fish in Central and Eastern of Algeria.. Acta Parasitol 2022 Sep;67(3):1152-1161.
- Ryan U, Zahedi A, Feng Y, Xiao L. An Update on Zoonotic Cryptosporidium Species and Genotypes in Humans.. Animals (Basel) 2021 Nov 19;11(11).
- Lebbad M, Winiecka-Krusnell J, Stensvold CR, Beser J. High Diversity of Cryptosporidium Species and Subtypes Identified in Cryptosporidiosis Acquired in Sweden and Abroad.. Pathogens 2021 Apr 26;10(5).
- Couso-Pérez S, de Limia FB, Ares-Mazás E, Gómez-Couso H. First report of zoonotic Cryptosporidium parvum GP60 subtypes IIaA15G2R1 and IIaA16G3R1 in wild ponies from the northern Iberian Peninsula.. Parasitol Res 2020 Jan;119(1):249-254.
- Hatam-Nahavandi K, Ahmadpour E, Carmena D, Spotin A, Bangoura B, Xiao L. Cryptosporidium infections in terrestrial ungulates with focus on livestock: a systematic review and meta-analysis.. Parasit Vectors 2019 Sep 14;12(1):453.
- Zhao W, Zhou H, Huang Y, Xu L, Rao L, Wang S, Wang W, Yi Y, Zhou X, Wu Y, Ma T, Wang G, Hu X, Peng R, Yin F, Lu G. Cryptosporidium spp. in wild rats (Rattus spp.) from the Hainan Province, China: Molecular detection, species/genotype identification and implications for public health.. Int J Parasitol Parasites Wildl 2019 Aug;9:317-321.
- Ouakli N, Belkhiri A, de Lucio A, Köster PC, Djoudi M, Dadda A, Khelef D, Kaidi R, Carmena D. Cryptosporidium-associated diarrhoea in neonatal calves in Algeria.. Vet Parasitol Reg Stud Reports 2018 May;12:78-84.
- Zahedi A, Paparini A, Jian F, Robertson I, Ryan U. Public health significance of zoonotic Cryptosporidium species in wildlife: Critical insights into better drinking water management.. Int J Parasitol Parasites Wildl 2016 Apr;5(1):88-109.
- Squire SA, Ryan U. Cryptosporidium and Giardia in Africa: current and future challenges.. Parasit Vectors 2017 Apr 20;10(1):195.
- Aldeyarbi HM, Abu El-Ezz NM, Karanis P. Cryptosporidium and cryptosporidiosis: the African perspective.. Environ Sci Pollut Res Int 2016 Jul;23(14):13811-21.
- Liu A, Zhang J, Zhao J, Zhao W, Wang R, Zhang L. The first report of Cryptosporidium andersoni in horses with diarrhea and multilocus subtype analysis.. Parasit Vectors 2015 Sep 22;8:483.
- Wagnerová P, Sak B, McEvoy J, Rost M, Matysiak AP, Ježková J, Kváč M. Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland.. Parasitol Res 2015 Apr;114(4):1619-24.
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