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Equine Doping Controls of Thymosin β $$ beta $$ 4: A Population Study and Strategy for Misuse Detection.
Abstract: Thymosin 4 (TB4) is a ubiquitous, highly conserved and abundant peptide among mammals with a critical role in cytoskeleton organization. In spite of its yet non-authorized use as a medicine and being forbidden by the IFHA, the FEI, and the WADA, intelligence and doping control laboratories reported numerous products available online claiming to contain a synthetic acetylated fragment of TB4 or TB4 itself, promoted as a growth factor with regenerative properties. In this article, the first estimation of the endogenous TB4 concentration in racing horses' blood samples was performed through a population study. We reveal that this concentration does not significantly depend on gender, age, nor horse breed. We highlight that the TB4 concentration increases significantly and rapidly in plasma stored at 4°C when not separated from blood cells due to cell lysis. Finally, we also demonstrate that the detection of a non-natural synthesis impurity is possible in equine plasma after a single dose administration of a TB4 containing product to a horse.
© 2024 John Wiley & Sons Ltd.
Publication Date: 2024-09-24 PubMed ID: 39314109DOI: 10.1002/dta.3806Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research study focuses on controlling the misuse of Thymosin β4 (TB4), a protein often claimed to promote growth and regeneration in horses, despite its unauthorized use in veterinary medicine. Through a population study, the research establishes base TB4 concentration levels in horse blood samples, showing these are not significantly influenced by gender, age, or breed, but notably increase when blood cells undergo lysis in stored plasma. The study also shows it is possible to detect synthetic impurities of TB4 in horse plasma following a single-dose administration.
Objective and Background of the Study
- The study was commissioned due to the presence of numerous TB4 products online, promoted as substances for growth and regeneration, despite TB4’s non-licensed use as a medical substance and restrictions imposed by major equine and anti-doping authorities.
- The researchers aimed to establish baseline TB4 levels in horses, which can serve as a point of reference in detecting possible misuse of this substance.
Approach of the Study
- A population study was conducted to emphasise the endogenous TB4 concentration in the blood samples of racing horses.
- The researchers examined if the levels of TB4 were influenced by the horse’s age, sex, and breed. They also investigated the change in TB4 levels during cell lysis (cell breakdown), which happens when plasma is stored at 4°C and not separated from the blood cells.
Key Findings
- The study identified that the TB4 concentration remained consistent, irrespective of the age, gender, or breed of the horse, thereby implying these factors don’t contribute significantly as variables during testing procedures.
- It was discovered that when blood cell lysis occurs during plasma storage at 4°C, the level of TB4 increases significantly. This finding is critical in ensuring appropriate handling and storage of samples for accurate results.
- The research also revealed that a synthetic impurity resulting from non-natural synthesis of TB4 can be detected in horse plasma after a single dose of a TB4 product is administered, establishing a method to detect potential misuse.
Cite This Article
APA
Delcourt V, Garcia P, Chabot B, Aber N, Pescher M, Cacault M, Scholtes P, Loup B, Barnabé A, Popot MA, Bailly-Chouriberry L.
(2024).
Equine Doping Controls of Thymosin β $$ beta $$ 4: A Population Study and Strategy for Misuse Detection.
Drug Test Anal.
https://doi.org/10.1002/dta.3806 Publication
Researcher Affiliations
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
- GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
Grant Funding
- Institut Franu00e7ais du Cheval et de l'Equitation
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