Equine endometrial vascular pattern changes during the estrous cycle examined by Narrow Band Imaging hysteroscopy.
Abstract: The aim of this study was to evaluate the uterine blood supply and endometrial vessel architecture, during the equine estrous cycle. Narrow Band Imaging (NBI) hysteroscopy was used for evaluating changes in the endometrial vasculature during the estrous cycle [six mares, d 0 (representing the day of ovulation), d 6 and 11 in four locations]. In addition, endometrial biopsy samples were used for immunodetection of markers for angiogenesis (Vascular Endothelial Growth Factor A, its receptor 2, as well as angiopoietin-2 and its receptor-tyrosine-kinase Tie2) during the estrous cycle (three mares, d 0, 5 and 10; one biopsy per mare). Detailed analysis of hysteroscopic images revealed an increase in the vascular density from estrus towards diestrus. In contrast, microscopic specimens prepared from biopsies revealed no evidence for changes in the endometrial vessel number during the estrous cycle. Studies on expression of angiogenesis markers indicated that cyclic changes in the endometrial vascular density observed by NBI-hysteroscopy were not due to formation of new vessels. It is concluded that vessels are involved in blood supply of a smaller area during diestrus, facilitating better distribution of nutrients during this phase.
Copyright © 2016 Elsevier B.V. All rights reserved.
Publication Date: 2016-01-06 PubMed ID: 26791330DOI: 10.1016/j.anireprosci.2016.01.006Google Scholar: Lookup
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- Journal Article
Summary
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This research looks at how the patterns of blood vessels in a horse’s uterus changes during its reproductive cycle. It used a specific type of imaging to monitor these changes and found an increase in the density of these vessels, although this did not seem to involve the creation of new vessels.
Study Aim and Methods
The research aimed to evaluate changes in the uterine blood supply and vessel architecture throughout a horse’s estrous cycle. This was accomplished by employing Narrow Band Imaging (NBI) hysteroscopy, which provides a detailed view of the uterine endometrium (internal lining) and the blood vessels within. This was done on six mares at three specific points in the estrous cycle (day 0, day 6, and day 11) in four different locations. Furthermore, endometrial biopsy samples were analyzed for markers of angiogenesis (the formation of new blood vessels) such as Vascular Endothelial Growth Factor A and its receptor, angiopoietin-2, and its receptor, tyrosine-kinase Tie2 at different points of the estrous cycle.
Hysteroscopic Observations
- Analyses of the hysteroscopic images indicated an increase in vascular density from estrus (the period of sexual receptivity prior to ovulation) to diestrus (the period following ovulation when the body prepares for potential pregnancy).
- However, microscopic examination of biopsied samples did not reveal evidence of increased blood vessels during the estrous cycle.
Investigations into Angiogenesis
- Research into the expression of angiogenesis markers revealed that the observed changes in endometrial vascular density during the estrous cycle did not result from the formation of new blood vessels.
Conclusion
- The research concludes that vessels are concentrated to supply a smaller area during diestrus. This could allow for a more efficient distribution of nutrients during this phase, which is often associated with preparation for potential pregnancy.
Cite This Article
APA
Otzen H, Sieme H, Oldenhof H, Kassens A, Ertmer F, Rode K, Müller K, Klose K, Rohn K, Schoon HA, Meinecke B.
(2016).
Equine endometrial vascular pattern changes during the estrous cycle examined by Narrow Band Imaging hysteroscopy.
Anim Reprod Sci, 166, 80-89.
https://doi.org/10.1016/j.anireprosci.2016.01.006 Publication
Researcher Affiliations
- Institute for Reproductive Biology, University of Veterinary Medicine Hannover, Bünteweg 2, 30559 Hannover, Germany. Electronic address: Henning.Otzen@tiho-hannover.de.
- Clinic for Horses-Unit for Reproductive Medicine, University of Veterinary Medicine Hannover, Bünteweg 15, 30559 Hannover, Germany. Electronic address: Harald.Sieme@tiho-hannover.de.
- Clinic for Horses-Unit for Reproductive Medicine, University of Veterinary Medicine Hannover, Bünteweg 15, 30559 Hannover, Germany. Electronic address: Harriette.Oldenhof@tiho-hannover.de.
- Clinic for Horses-Unit for Reproductive Medicine, University of Veterinary Medicine Hannover, Bünteweg 15, 30559 Hannover, Germany. Electronic address: Ana.Kassens@tiho-hannover.de.
- Clinic for Horses-Unit for Reproductive Medicine, University of Veterinary Medicine Hannover, Bünteweg 15, 30559 Hannover, Germany. Electronic address: Franziska.Ertmer@tiho-hannover.de.
- Institute for Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany. Electronic address: Kristina.Rode@tiho-hannover.de.
- Institute of Pathology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 33, 04103 Leipzig, Germany. Electronic address: kristin.mueller@vetmed.uni-leipzig.de.
- Institute of Pathology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 33, 04103 Leipzig, Germany. Electronic address: kristin.klose@vetmed.uni-leipzig.de.
- Institute for Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Bünteweg 2, 30559 Hannover, Germany. Electronic address: Karl.Rohn@tiho-hannover.de.
- Institute of Pathology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 33, 04103 Leipzig, Germany. Electronic address: schoon@vetmed.uni-leipzig.de.
- Institute for Reproductive Biology, University of Veterinary Medicine Hannover, Bünteweg 2, 30559 Hannover, Germany. Electronic address: Burkhard.Meinecke@tiho-hannover.de.
MeSH Terms
- Angiopoietin-2 / metabolism
- Animals
- Endometrium / blood supply
- Endometrium / diagnostic imaging
- Endometrium / metabolism
- Endometrium / pathology
- Estrous Cycle / physiology
- Female
- Horses / metabolism
- Hysteroscopy / methods
- Hysteroscopy / veterinary
- Narrow Band Imaging / veterinary
- Pregnancy
- Receptor, TIE-2 / metabolism
- Uterus / blood supply
- Uterus / diagnostic imaging
- Vascular Endothelial Growth Factor A / metabolism
- Vascular Endothelial Growth Factor Receptor-2 / metabolism
Citations
This article has been cited 1 times.- Piotrowska-Tomala KK, Jonczyk AW, Szóstek-Mioduchowska AZ, Żebrowska E, Ferreira-Dias G, Skarzynski DJ. The Effects of Prostaglandin E(2) Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study.. Front Vet Sci 2021;8:753796.
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