Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
Abstract: Endometrosis is an important mares' disease which considerably decreases their fertility. As classic endometrial classification methods might be insufficient for tissue pathological evaluation, further categorization into active/inactive and destructive/non-destructive types was developed by Hoffmann and others. This study aimed to compare NF-κB pathway genes transcription among histopathological types of endometrosis, following Hoffmann and co-authors' classification. Endometrial samples, collected postmortem from cyclic mares ( = 100) in estrus or diestrus, were classified histologically and used for gene transcription assessment. Gene transcription of NF-κB subunits (, , ), pro-inflammatory molecules (, ), and hyaluronan synthases (, , ) was compared among endometrosis types (active, non-active, destructive, non-destructive). Most individual mRNA samples showed high expression of , , and gene transcripts and the destructive type of endometrosis, simultaneously. The expression of and genes was higher in active destructive group than in the other groups only in the follicular phase, as well as being higher in the inactive destructive group than in the others, only in the mid-luteal phase. The increase in gene transcription of the NF-κB canonical activation pathway in destructive endometrosis may suggest the highest changes in extracellular matrix deposition. Moreover, the estrous cycle phase might influence fibrosis pathogenesis.
Publication Date: 2021-11-04 PubMed ID: 34827882PubMed Central: PMC8614257DOI: 10.3390/ani11113151Google Scholar: Lookup
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Summary
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This study investigates whether the transcription of certain genes in the NF-κB pathway differ between different pathological types of a mare fertility disease called endometrosis. Additionally, the research evaluates how the phase of the estrous cycle may influence this disease development.
Background
- Endometrosis is a fertility disease in mares that is classified in various ways for pathological evaluation. Hoffmann and others introduced a classification method that separates the disease into active/inactive and destructive/non-destructive types.
- This study focuses on the NF-κB pathway, a protein complex that plays a key role in regulating immune response to infection. It is often inactive in healthy cells and is activated to combat infection.
Purpose and Methods
- The purpose of this study was to compare the transcription of genes in the NF-κB pathway among the different pathological types of endometrosis. This comparison was carried out based on Hoffmann’s classification method.
- Endometrial samples were collected from mares in different stages of their estrous cycle and were then examined for gene transcription.
Findings
- The results revealed that most of the mRNA samples showed high expression of certain gene transcripts, including those in the NF-κB subunits and pro-inflammatory molecules,
- Such high expressions were mostly prevalent in the destructive type of endometrosis.
- The expression of certain genes was higher in the active destructive group than in other groups, and this was only found in the follicular phase.
Conclusion
- The study concludes that the transcription of genes in the NF-κB pathway may be influenced by the classification of endometrosis and the phase of the estrous cycle the mare is in.
- It suggests that the highest changes in extracellular matrix deposition might correspond to the increased gene transcription in destructive endometrosis.
Cite This Article
APA
Jasiński T, Zdrojkowski Ł, Kautz E, Juszczuk-Kubiak E, Ferreira-Dias G, Domino M.
(2021).
Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
Animals (Basel), 11(11), 3151.
https://doi.org/10.3390/ani11113151 Publication
Researcher Affiliations
- Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
- Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
- Department of Molecular Biology, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, 05-552 Jastrzębiec, Poland.
- Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology-State Research Institute, 02-532 Warsaw, Poland.
- Departmento de Morfologia e Função, CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-47 Lisbon, Portugal.
- Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Grant Funding
- 2018/02/X/NZ4/00101 / National Science Center
Conflict of Interest Statement
The authors declare no conflict of interest.
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