Equine herpesvirus 1 sequence near the left terminus codes for two open reading frames.
Abstract: We have previously reported the sequence of the equine herpesvirus one genomic termini that are homologous to the genomic termini of other herpesviruses. In this paper, we present the nucleotide sequence adjacent to the left terminus sequence (map units 0.0087 to 0.0237). This sequence codes for two open reading frames (ORF) which are homologous to ORF2 and ORF3 of the varicella-zoster virus genome and are located at colinear positions. The L region sequence presented here also contains a segment that is involved in the generation of the genome of EHV-1 DI particles through recombination with sequences mapping within the internal portion of the inverted repeat sequences of the short region.
Publication Date: 1991-03-01 PubMed ID: 1645901DOI: 10.1016/0168-1702(91)90012-kGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The article investigates the DNA sequence near the left end of the equine herpesvirus 1 (EHV-1), identifying two gene-coding regions which are analogous to genes found in the varicella-zoster virus genome.
Study Background
- The study continues the researchers’ prior work, where they reported on sequences of the EHV-1 genome corresponding to sequences in other herpesvirus genomes.
Study Focus and Findings
- In this paper, the focus is on the DNA sequence next to the left terminus of the EHV-1 genome (specifically the sequence from 0.0087 to 0.0237 map units).
- The researchers discovered that this sequence encodes for two open reading frames (ORF), which are sections of the genome where the information contained in the DNA is translated into proteins.
- These ORFs have homology to ORF2 and ORF3 of the varicella-zoster virus genome, i.e., they have a similar sequence and are therefore likely to perform similar functions within the virus.
- Furthermore, these ORFs are also found at the same locations (colinear positions) in both the EHV-1 and varicella-zoster virus genomes, strengthening the likelihood of their analogous function.
Genome Generation of EHV-1
- The study also revealed that this particular sequence of the EHV-1 genome contains a segment responsible for generating more of the EHV-1 genome in defective interfering (DI) viral particles.
- This is achieved via recombination with sequences within the internal section of inverted repeat sequences in the short region of the EHV-1 genome.
Cite This Article
APA
Yalamanchili RR, Raengsakulrach B, O'Callaghan DJ.
(1991).
Equine herpesvirus 1 sequence near the left terminus codes for two open reading frames.
Virus Res, 18(2-3), 109-116.
https://doi.org/10.1016/0168-1702(91)90012-k Publication
Researcher Affiliations
- Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cell Line
- Cloning, Molecular
- Defective Viruses / genetics
- Herpesvirus 1, Equid / genetics
- Molecular Sequence Data
- Open Reading Frames / genetics
- Recombination, Genetic
- Repetitive Sequences, Nucleic Acid / genetics
- Sequence Homology, Nucleic Acid
- Transcription, Genetic
Grant Funding
- AI 22001 / NIAID NIH HHS
Citations
This article has been cited 16 times.- Schnabel CL, Wimer CL, Perkins G, Babasyan S, Freer H, Watts C, Rollins A, Osterrieder N, Wagner B. Deletion of the ORF2 gene of the neuropathogenic equine herpesvirus type 1 strain Ab4 reduces virulence while maintaining strong immunogenicity.. BMC Vet Res 2018 Aug 22;14(1):245.
- Shakya AK, O'Callaghan DJ, Kim SK. Comparative Genomic Sequencing and Pathogenic Properties of Equine Herpesvirus 1 KyA and RacL11.. Front Vet Sci 2017;4:211.
- Kim SK, Shakya AK, O'Callaghan DJ. Immunization with Attenuated Equine Herpesvirus 1 Strain KyA Induces Innate Immune Responses That Protect Mice from Lethal Challenge.. J Virol 2016 Sep 15;90(18):8090-104.
- Ahn BC, Kim S, Zhang Y, Charvat RA, O'Callaghan DJ. The early UL3 gene of equine herpesvirus-1 encodes a tegument protein not essential for replication or virulence in the mouse.. Virology 2011 Nov 10;420(1):20-31.
- Ebner PD, Kim SK, O'Callaghan DJ. Biological and genotypic properties of defective interfering particles of equine herpesvirus 1 that mediate persistent infection.. Virology 2008 Nov 10;381(1):98-105.
- Ebner PD, O'Callaghan DJ. Genetic complexity of EHV-1 defective interfering particles and identification of novel IR4/UL5 hybrid proteins produced during persistent infection.. Virus Genes 2006 Jun;32(3):313-20.
- Smith PM, Zhang Y, Grafton WD, Jennings SR, O'Callaghan DJ. Severe murine lung immunopathology elicited by the pathogenic equine herpesvirus 1 strain RacL11 correlates with early production of macrophage inflammatory proteins 1alpha, 1beta, and 2 and tumor necrosis factor alpha.. J Virol 2000 Nov;74(21):10034-40.
- Bowles DE, Holden VR, Zhao Y, O'Callaghan DJ. The ICP0 protein of equine herpesvirus 1 is an early protein that independently transactivates expression of all classes of viral promoters.. J Virol 1997 Jul;71(7):4904-14.
- Chen M, Harty RN, Zhao Y, Holden VR, O'Callaghan DJ. Expression of an equine herpesvirus 1 ICP22/ICP27 hybrid protein encoded by defective interfering particles associated with persistent infection.. J Virol 1996 Jan;70(1):313-20.
- Harty RN, Caughman GB, Holden VR, O'Callaghan DJ. Characterization of the myristylated polypeptide encoded by the UL1 gene that is conserved in the genome of defective interfering particles of equine herpesvirus 1.. J Virol 1993 Jul;67(7):4122-32.
- Harty RN, Holden VR, O'Callaghan DJ. Transcriptional and translational analyses of the UL2 gene of equine herpesvirus 1: a homolog of UL55 of herpes simplex virus type 1 that is maintained in the genome of defective interfering particles.. J Virol 1993 Apr;67(4):2255-65.
- Fraefel C, Wirth UV, Vogt B, Schwyzer M. Immediate-early transcription over covalently joined genome ends of bovine herpesvirus 1: the circ gene.. J Virol 1993 Mar;67(3):1328-33.
- Smith RH, Zhao Y, O'Callaghan DJ. The equine herpesvirus 1 (EHV-1) UL3 gene, an ICP27 homolog, is necessary for full activation of gene expression directed by an EHV-1 late promoter.. J Virol 1993 Feb;67(2):1105-9.
- Fraefel C, Ackermann M, Schwyzer M. Identification of the bovine herpesvirus 1 circ protein, a myristylated and virion-associated polypeptide which is not essential for virus replication in cell culture.. J Virol 1994 Dec;68(12):8082-8.
- Baumeister J, Klupp BG, Mettenleiter TC. Pseudorabies virus and equine herpesvirus 1 share a nonessential gene which is absent in other herpesviruses and located adjacent to a highly conserved gene cluster.. J Virol 1995 Sep;69(9):5560-7.
- Zhao Y, Holden VR, Harty RN, O'Callaghan DJ. Identification and transcriptional analyses of the UL3 and UL4 genes of equine herpesvirus 1, homologs of the ICP27 and glycoprotein K genes of herpes simplex virus.. J Virol 1992 Sep;66(9):5363-72.
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