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Home / Equine Research Bank / Stem Cell Reports / Equine-Induced Pluripotent Stem Cells Retain Lineage Commitm...
Stem cell reports2016; 6(1); 55-63; doi: 10.1016/j.stemcr.2015.12.005

Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates.

Abstract: Induced pluripotent stem cells (iPSCs) hold great potential not only for human but also for veterinary purposes. The equine industry must often deal with health issues concerning muscle and cartilage, where comprehensive regenerative strategies are still missing. In this regard, a still open question is whether equine iPSCs differentiate toward muscle and cartilage, and whether donor cell type influences their differentiation potential. We addressed these questions through an isogenic system of equine iPSCs obtained from myogenic mesoangioblasts (MAB-iPSCs) and chondrogenic mesenchymal stem cells (MSC-iPSCs). Despite similar levels of pluripotency characteristics, the myogenic differentiation appeared enhanced in MAB-iPSCs. Conversely, the chondrogenic differentiation was augmented in MSC-iPSCs through both teratoma and in vitro differentiation assays. Thus, our data suggest that equine iPSCs can differentiate toward the myogenic and chondrogenic lineages, and can present a skewed differentiation potential in favor of the source cell lineage.
Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
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Publication Date: 2016-01-16 PubMed ID: 26771353PubMed Central: PMC4719186DOI: 10.1016/j.stemcr.2015.12.005Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on understanding the potential of equine-induced pluripotent stem cells (iPSCs) to differentiate towards muscle and cartilage cell types, to provide regenerative strategies for muscle and cartilage related health issues in the equine industry.

Objective

  • The objective of the study was to determine whether equine iPSCs could differentiate towards muscle and cartilage and whether the type of donor cell influenced this differentiation process.

Method

  • The researchers used an isogenic system of equine iPSCs obtained from myogenic mesoangioblasts (MAB-iPSCs) and chondrogenic mesenchymal stem cells (MSC-iPSCs).
  • They analyzed the levels of pluripotency characteristics in these iPS cells and conducted differentiation assays both on teratoma and in vitro to compare the potentials of these cells to differentiate into muscle and cartilage cells.

Findings

  • The analysis revealed that even though the cells displayed similar levels of pluripotency characteristics, MAB-iPSCs showed enhanced differentiation towards myogenic cells, while MSC-iPSCs demonstrated increased chondrogenic differentiation.
  • These findings led the researchers to conclude that while equine iPSCs can differentiate towards both myogenic and chondrogenic lineages, the differentiation potential is skewed towards the cell lineage of the source cells.

Implication

  • This study brings to light important insights into the potential of iPSCs in regenerative strategies for treating muscle and cartilage health issues in the equine industry.
  • The findings indicate the impact of the source cell lineage in determining the differentiation potential and suggest that selecting the appropriate source cells could enhance the efficacy of stem cell therapies.

Cite This Article

APA
Quattrocelli M, Giacomazzi G, Broeckx SY, Ceelen L, Bolca S, Spaas JH, Sampaolesi M. (2016). Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates. Stem Cell Reports, 6(1), 55-63. https://doi.org/10.1016/j.stemcr.2015.12.005

Publication

Stem cell reports
ISSN: 2213-6711
NlmUniqueID: 101611300
Country: United States
Language: English
Volume: 6
Issue: 1
Pages: 55-63
PII: S2213-6711(15)00370-7

Researcher Affiliations

Quattrocelli, Mattia
  • Translational Cardiomyology Lab, Stem Cell Biology and Embryology Unit, Department Development and Regeneration, KU Leuven, 3000 Leuven, Belgium.
Giacomazzi, Giorgia
  • Translational Cardiomyology Lab, Stem Cell Biology and Embryology Unit, Department Development and Regeneration, KU Leuven, 3000 Leuven, Belgium.
Broeckx, Sarah Y
  • Global Stem Cell Technology, ANACURA Group, 9940 Evergem, Belgium.
Ceelen, Liesbeth
  • Pathlicon, ANACURA Group, 9940 Evergem, Belgium.
Bolca, Selin
  • Pathlicon, ANACURA Group, 9940 Evergem, Belgium.
Spaas, Jan H
  • Global Stem Cell Technology, ANACURA Group, 9940 Evergem, Belgium. Electronic address: jan.spaas@anacura.com.
Sampaolesi, Maurilio
  • Translational Cardiomyology Lab, Stem Cell Biology and Embryology Unit, Department Development and Regeneration, KU Leuven, 3000 Leuven, Belgium; Division of Human Anatomy, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, 27100 Pavia, Italy. Electronic address: maurilio.sampaolesi@med.kuleuven.be.

MeSH Terms

  • Animals
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • Cell Line
  • Cell Lineage / genetics
  • Cell Lineage / physiology
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrocytes / metabolism
  • Chondrogenesis / genetics
  • Chondrogenesis / physiology
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Horses
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / metabolism
  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors / genetics
  • Kruppel-Like Transcription Factors / metabolism
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Muscle Development / genetics
  • Muscle Development / physiology
  • Octamer Transcription Factor-3 / genetics
  • Octamer Transcription Factor-3 / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • SOXB1 Transcription Factors / genetics
  • SOXB1 Transcription Factors / metabolism

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Citations

This article has been cited 11 times.
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